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Direct electrochemistry and electrocatalytic properties of hemoglobin immobilized on a carbon ionic liquid electrode modified with mesoporous molecular sieve MCM-41.
Colloids Surf B Biointerfaces. 2010 Aug 01; 79(1):241-5.CS

Abstract

The direct electron transfer and electrocatalysis of hemoglobin (Hb) entrapped in the MCM-41 modified carbon ionic liquid electrode (CILE) were investigated by using cyclic voltammetry in 0.10 M pH 7.0 phosphate buffer solution (PBS). Due to its uniform pore structure, high surface areas and good biocompatibility, the mesoporous silica sieve MCM-41 provided a suitable matrix for immobilization of biomolecule. The MCM-41 modified CILE showed significant promotion to the direct electron transfer of Hb, which exhibited a pair of well defined and quasi-reversible peaks for heme Fe(III)/Fe(II) with a formal potential of -0.284 V (vs. Ag/AgCl). Additionally, the Hb immobilized on the MCM-41 modified carbon ionic liquid electrode showed excellent electrocatalytic activity toward H(2)O(2). The electrocatalytic current values were linear with increasing concentration of H(2)O(2) in a wide range of 5-310 microM and the corresponding detection limit was calculated to be 5 x 10(-8)M (S/N=3). The surface coverage of Hb immobilized on the MCM-41 modified carbon ionic liquid electrode was about 2.54 x 10(-9) molcm(-2). The Michaelis-Menten constant K(m)(app) of 214 microM indicated that the Hb immobilized on the modified electrode showed high affinity to H(2)O(2). The proposed electrode had high stability and good reproducibility due to the protection effect of MCM-41 and ionic liquid, and it would have wide potential applications in direct electrochemistry, biosensors and biocatalysis.

Authors+Show Affiliations

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Hunan, Changsha 410082, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20430597

Citation

Li, Yonghong, et al. "Direct Electrochemistry and Electrocatalytic Properties of Hemoglobin Immobilized On a Carbon Ionic Liquid Electrode Modified With Mesoporous Molecular Sieve MCM-41." Colloids and Surfaces. B, Biointerfaces, vol. 79, no. 1, 2010, pp. 241-5.
Li Y, Zeng X, Liu X, et al. Direct electrochemistry and electrocatalytic properties of hemoglobin immobilized on a carbon ionic liquid electrode modified with mesoporous molecular sieve MCM-41. Colloids Surf B Biointerfaces. 2010;79(1):241-5.
Li, Y., Zeng, X., Liu, X., Liu, X., Wei, W., & Luo, S. (2010). Direct electrochemistry and electrocatalytic properties of hemoglobin immobilized on a carbon ionic liquid electrode modified with mesoporous molecular sieve MCM-41. Colloids and Surfaces. B, Biointerfaces, 79(1), 241-5. https://doi.org/10.1016/j.colsurfb.2010.04.001
Li Y, et al. Direct Electrochemistry and Electrocatalytic Properties of Hemoglobin Immobilized On a Carbon Ionic Liquid Electrode Modified With Mesoporous Molecular Sieve MCM-41. Colloids Surf B Biointerfaces. 2010 Aug 1;79(1):241-5. PubMed PMID: 20430597.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct electrochemistry and electrocatalytic properties of hemoglobin immobilized on a carbon ionic liquid electrode modified with mesoporous molecular sieve MCM-41. AU - Li,Yonghong, AU - Zeng,Xiandong, AU - Liu,Xiaoying, AU - Liu,Xinsheng, AU - Wei,Wanzhi, AU - Luo,Shenglian, Y1 - 2010/04/14/ PY - 2009/09/27/received PY - 2010/04/05/revised PY - 2010/04/07/accepted PY - 2010/5/1/entrez PY - 2010/5/1/pubmed PY - 2010/8/21/medline SP - 241 EP - 5 JF - Colloids and surfaces. B, Biointerfaces JO - Colloids Surf B Biointerfaces VL - 79 IS - 1 N2 - The direct electron transfer and electrocatalysis of hemoglobin (Hb) entrapped in the MCM-41 modified carbon ionic liquid electrode (CILE) were investigated by using cyclic voltammetry in 0.10 M pH 7.0 phosphate buffer solution (PBS). Due to its uniform pore structure, high surface areas and good biocompatibility, the mesoporous silica sieve MCM-41 provided a suitable matrix for immobilization of biomolecule. The MCM-41 modified CILE showed significant promotion to the direct electron transfer of Hb, which exhibited a pair of well defined and quasi-reversible peaks for heme Fe(III)/Fe(II) with a formal potential of -0.284 V (vs. Ag/AgCl). Additionally, the Hb immobilized on the MCM-41 modified carbon ionic liquid electrode showed excellent electrocatalytic activity toward H(2)O(2). The electrocatalytic current values were linear with increasing concentration of H(2)O(2) in a wide range of 5-310 microM and the corresponding detection limit was calculated to be 5 x 10(-8)M (S/N=3). The surface coverage of Hb immobilized on the MCM-41 modified carbon ionic liquid electrode was about 2.54 x 10(-9) molcm(-2). The Michaelis-Menten constant K(m)(app) of 214 microM indicated that the Hb immobilized on the modified electrode showed high affinity to H(2)O(2). The proposed electrode had high stability and good reproducibility due to the protection effect of MCM-41 and ionic liquid, and it would have wide potential applications in direct electrochemistry, biosensors and biocatalysis. SN - 1873-4367 UR - https://www.unboundmedicine.com/medline/citation/20430597/Direct_electrochemistry_and_electrocatalytic_properties_of_hemoglobin_immobilized_on_a_carbon_ionic_liquid_electrode_modified_with_mesoporous_molecular_sieve_MCM_41_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0927-7765(10)00204-3 DB - PRIME DP - Unbound Medicine ER -