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[Determination of effective components in different positions of Panax notoginseng by HPLC].
Zhong Yao Cai. 2009 Dec; 32(12):1810-3.ZY

Abstract

OBJECTIVE

A quantitative method was developed by gradient elution for the determination of notoginsenoside R1, ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 in different positions of Panax Notoginseng by HPLC. The content of 4 kinds saponins in different positions of Panax Notoginseng were compared.

METHODS

The different positions of Panax notoginseng (including root, rhizome, branch root, leaf, flower) were extracted with methanol. The HPLC condition was as following: Kromasil C18 column (250 mm x 4.6 mm, 5 microm), acetonitrile and water linearity gradient elution, flow rate at 1.0 mL/min, column temperature at 25 degrees C, wavelength 203 nm.

RESULTS

The linear ranges of notoginsenoside R1, ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 were 4.4-440 microg/mL, 4.32-1080 microg/mL, 4.24-212 microg/mL and 4.48-1120 microg/mL, respectively. The RSD (n=5) of average contents of intra-day and inter-day of 4 kinds saponins were 0.46%, 0.24%, 0.77%, 0.68% and 1.64%, 0.69%, 0.52%, 0.65%, respectively. The average recoveries were (102.93 +/- 1.22)%, (103.18 +/- 0.49)%, (103.20 +/- 1.58)%, (103.86 +/- 0.39)%, respectively. The content of 4 kinds saponins in different position of Panax notoginseng was: rhizome > root > branch root > flower > leaf; the content of 4 kinds saponin in the root of Panax notoginseng was: 80 pieces in 500 g >60 pieces in 500 g >20 pieces in 500 g >40 pieces in 500 g >100 pieces in 500 g.

CONCLUSION

This method is simple, sensitive, accurate and repeat, and is suitable in determination of the content of 4 kinds saponins in different positions of Panax notoginseng.

Authors+Show Affiliations

Guang'anmen Hospital, China Academy of Chinese Medical Science, Beijing 100053, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

20432895

Citation

Cui, Han-ming, et al. "[Determination of Effective Components in Different Positions of Panax Notoginseng By HPLC]." Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, vol. 32, no. 12, 2009, pp. 1810-3.
Cui HM, Zhang CG, Lin H, et al. [Determination of effective components in different positions of Panax notoginseng by HPLC]. Zhong Yao Cai. 2009;32(12):1810-3.
Cui, H. M., Zhang, C. G., Lin, H., Lu, W. L., Cheng, H. P., & Wang, J. (2009). [Determination of effective components in different positions of Panax notoginseng by HPLC]. Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, 32(12), 1810-3.
Cui HM, et al. [Determination of Effective Components in Different Positions of Panax Notoginseng By HPLC]. Zhong Yao Cai. 2009;32(12):1810-3. PubMed PMID: 20432895.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Determination of effective components in different positions of Panax notoginseng by HPLC]. AU - Cui,Han-ming, AU - Zhang,Chun-guang, AU - Lin,Hai, AU - Lu,Wen-long, AU - Cheng,Hui-ping, AU - Wang,Jie, PY - 2010/5/4/entrez PY - 2010/5/4/pubmed PY - 2011/5/17/medline SP - 1810 EP - 3 JF - Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials JO - Zhong Yao Cai VL - 32 IS - 12 N2 - OBJECTIVE: A quantitative method was developed by gradient elution for the determination of notoginsenoside R1, ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 in different positions of Panax Notoginseng by HPLC. The content of 4 kinds saponins in different positions of Panax Notoginseng were compared. METHODS: The different positions of Panax notoginseng (including root, rhizome, branch root, leaf, flower) were extracted with methanol. The HPLC condition was as following: Kromasil C18 column (250 mm x 4.6 mm, 5 microm), acetonitrile and water linearity gradient elution, flow rate at 1.0 mL/min, column temperature at 25 degrees C, wavelength 203 nm. RESULTS: The linear ranges of notoginsenoside R1, ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 were 4.4-440 microg/mL, 4.32-1080 microg/mL, 4.24-212 microg/mL and 4.48-1120 microg/mL, respectively. The RSD (n=5) of average contents of intra-day and inter-day of 4 kinds saponins were 0.46%, 0.24%, 0.77%, 0.68% and 1.64%, 0.69%, 0.52%, 0.65%, respectively. The average recoveries were (102.93 +/- 1.22)%, (103.18 +/- 0.49)%, (103.20 +/- 1.58)%, (103.86 +/- 0.39)%, respectively. The content of 4 kinds saponins in different position of Panax notoginseng was: rhizome > root > branch root > flower > leaf; the content of 4 kinds saponin in the root of Panax notoginseng was: 80 pieces in 500 g >60 pieces in 500 g >20 pieces in 500 g >40 pieces in 500 g >100 pieces in 500 g. CONCLUSION: This method is simple, sensitive, accurate and repeat, and is suitable in determination of the content of 4 kinds saponins in different positions of Panax notoginseng. SN - 1001-4454 UR - https://www.unboundmedicine.com/medline/citation/20432895/[Determination_of_effective_components_in_different_positions_of_Panax_notoginseng_by_HPLC]_ L2 - https://antibodies.cancer.gov/detail/CPTC-RB1-1 DB - PRIME DP - Unbound Medicine ER -