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Luminol/antibody labeled gold nanoparticles for chemiluminescence immunoassay of carcinoembryonic antigen.
Anal Chim Acta. 2010 May 07; 666(1-2):91-6.AC

Abstract

A facile strategy by loading luminol and secondary antibody on gold nanoparticles (Au NPs) was described in the present work. The as-prepared luminol/antibody labeled Au NPs conjugates (LAAu NPs) were used as the chemiluminescent probe for the detection of carcinoembryonic antigen (CEA) in serum. The LAAu NPs were characterized by transmission electron microscopy (TEM), UV-vis spectrophotometry (UV-vis), and chemiluminescent method. Stable and efficient chemiluminescence (CL) was obtained when luminol molecules and secondary antibodies were coimmobilized on the Au NPs by using hydrogen peroxide (H(2)O(2)) as an oxidant, horseradish peroxidase (HRP) as a catalyst, and 4-(4'-iodo)phenylphenol (IPP) as an enhancer. The LAAu NPs were further evaluated via a sandwich-type CL immunoassay of CEA in serum. In this protocol, the CEA analyte was captured by the primary antibody immobilized on the surface of magnetic beads, and then was sandwiched by the secondary antibody loaded on luminol-labeled Au NPs. The chemiluminescent intensity was proportional to the concentration of CEA over the range of 5.0x10(-10) to 5.0x10(-8) g mL(-1) and 5.0x10(-9) to 2.0x10(-8) g mL(-1) by using HRP and Co(2+) as catalysts, respectively. The present chemiluminescent immunoassay based on the luminol/antibody labeled Au NPs conjugates has offered great promise for simple, highly biocompatible, and cost-effective analysis of biological samples.

Authors+Show Affiliations

Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. yangxiaoyan zh@126.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20433970

Citation

Yang, Xiaoyan, et al. "Luminol/antibody Labeled Gold Nanoparticles for Chemiluminescence Immunoassay of Carcinoembryonic Antigen." Analytica Chimica Acta, vol. 666, no. 1-2, 2010, pp. 91-6.
Yang X, Guo Y, Wang A. Luminol/antibody labeled gold nanoparticles for chemiluminescence immunoassay of carcinoembryonic antigen. Anal Chim Acta. 2010;666(1-2):91-6.
Yang, X., Guo, Y., & Wang, A. (2010). Luminol/antibody labeled gold nanoparticles for chemiluminescence immunoassay of carcinoembryonic antigen. Analytica Chimica Acta, 666(1-2), 91-6. https://doi.org/10.1016/j.aca.2010.03.059
Yang X, Guo Y, Wang A. Luminol/antibody Labeled Gold Nanoparticles for Chemiluminescence Immunoassay of Carcinoembryonic Antigen. Anal Chim Acta. 2010 May 7;666(1-2):91-6. PubMed PMID: 20433970.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Luminol/antibody labeled gold nanoparticles for chemiluminescence immunoassay of carcinoembryonic antigen. AU - Yang,Xiaoyan, AU - Guo,Yingshu, AU - Wang,Aiguo, Y1 - 2010/04/04/ PY - 2010/02/05/received PY - 2010/03/27/revised PY - 2010/03/29/accepted PY - 2010/5/4/entrez PY - 2010/5/4/pubmed PY - 2010/9/21/medline SP - 91 EP - 6 JF - Analytica chimica acta JO - Anal Chim Acta VL - 666 IS - 1-2 N2 - A facile strategy by loading luminol and secondary antibody on gold nanoparticles (Au NPs) was described in the present work. The as-prepared luminol/antibody labeled Au NPs conjugates (LAAu NPs) were used as the chemiluminescent probe for the detection of carcinoembryonic antigen (CEA) in serum. The LAAu NPs were characterized by transmission electron microscopy (TEM), UV-vis spectrophotometry (UV-vis), and chemiluminescent method. Stable and efficient chemiluminescence (CL) was obtained when luminol molecules and secondary antibodies were coimmobilized on the Au NPs by using hydrogen peroxide (H(2)O(2)) as an oxidant, horseradish peroxidase (HRP) as a catalyst, and 4-(4'-iodo)phenylphenol (IPP) as an enhancer. The LAAu NPs were further evaluated via a sandwich-type CL immunoassay of CEA in serum. In this protocol, the CEA analyte was captured by the primary antibody immobilized on the surface of magnetic beads, and then was sandwiched by the secondary antibody loaded on luminol-labeled Au NPs. The chemiluminescent intensity was proportional to the concentration of CEA over the range of 5.0x10(-10) to 5.0x10(-8) g mL(-1) and 5.0x10(-9) to 2.0x10(-8) g mL(-1) by using HRP and Co(2+) as catalysts, respectively. The present chemiluminescent immunoassay based on the luminol/antibody labeled Au NPs conjugates has offered great promise for simple, highly biocompatible, and cost-effective analysis of biological samples. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/20433970/Luminol/antibody_labeled_gold_nanoparticles_for_chemiluminescence_immunoassay_of_carcinoembryonic_antigen_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(10)00369-7 DB - PRIME DP - Unbound Medicine ER -