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Synthesis and characterization of a molecularly imprinted polymer and its application as SPE enrichment sorbent for determination of trace methimazole in pig samples using HPLC-UV.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Jun 01; 878(19):1531-6.JC

Abstract

A novel molecularly imprinted polymer that could be applied as enrichment sorbent was prepared using methimazole (MMZ) as the template molecule, methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linker. Though evaluated by static, kinetic and competitive adsorption tests, the polymer exhibited high adsorption capacity, fast kinetics and good selective ability. A method for determination of trace MMZ was developed using this polymer as enrichment sorbent coupled with high performance liquid chromatography focusing on complex biological matrices. Under the optimum experimental conditions, the MMZ standard is linear within the concentration range studied, that is, from 0.5 microg L(-1) to 150 microg L(-1) (r2=0.9941). Lower limits of detection (LOD, at S/N=3) and quantification (LOQ, at S/N=10) in pig samples were 0.63 microg kg(-1) and 2.10 microg kg(-1) for kidney, 0.51 microg kg(-1) and 1.70 microg kg(-1) for liver, 0.56 microg kg(-1) and 1.86 microg kg(-1) for muscle, respectively. Recoveries and relative standard deviation (RSD, n=9) values for precision in the developed method were from 71.14% to 88.41% and from 2.53% to 6.18%.

Authors+Show Affiliations

Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin, 300457, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20434412

Citation

Pan, Mingfei, et al. "Synthesis and Characterization of a Molecularly Imprinted Polymer and Its Application as SPE Enrichment Sorbent for Determination of Trace Methimazole in Pig Samples Using HPLC-UV." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 878, no. 19, 2010, pp. 1531-6.
Pan M, Wang J, Fang G, et al. Synthesis and characterization of a molecularly imprinted polymer and its application as SPE enrichment sorbent for determination of trace methimazole in pig samples using HPLC-UV. J Chromatogr B Analyt Technol Biomed Life Sci. 2010;878(19):1531-6.
Pan, M., Wang, J., Fang, G., Tang, W., & Wang, S. (2010). Synthesis and characterization of a molecularly imprinted polymer and its application as SPE enrichment sorbent for determination of trace methimazole in pig samples using HPLC-UV. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 878(19), 1531-6. https://doi.org/10.1016/j.jchromb.2010.04.004
Pan M, et al. Synthesis and Characterization of a Molecularly Imprinted Polymer and Its Application as SPE Enrichment Sorbent for Determination of Trace Methimazole in Pig Samples Using HPLC-UV. J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Jun 1;878(19):1531-6. PubMed PMID: 20434412.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synthesis and characterization of a molecularly imprinted polymer and its application as SPE enrichment sorbent for determination of trace methimazole in pig samples using HPLC-UV. AU - Pan,Mingfei, AU - Wang,Junping, AU - Fang,Guozhen, AU - Tang,Weiwei, AU - Wang,Shuo, Y1 - 2010/04/14/ PY - 2009/11/24/received PY - 2010/03/23/revised PY - 2010/04/06/accepted PY - 2010/5/4/entrez PY - 2010/5/4/pubmed PY - 2010/8/27/medline SP - 1531 EP - 6 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 878 IS - 19 N2 - A novel molecularly imprinted polymer that could be applied as enrichment sorbent was prepared using methimazole (MMZ) as the template molecule, methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linker. Though evaluated by static, kinetic and competitive adsorption tests, the polymer exhibited high adsorption capacity, fast kinetics and good selective ability. A method for determination of trace MMZ was developed using this polymer as enrichment sorbent coupled with high performance liquid chromatography focusing on complex biological matrices. Under the optimum experimental conditions, the MMZ standard is linear within the concentration range studied, that is, from 0.5 microg L(-1) to 150 microg L(-1) (r2=0.9941). Lower limits of detection (LOD, at S/N=3) and quantification (LOQ, at S/N=10) in pig samples were 0.63 microg kg(-1) and 2.10 microg kg(-1) for kidney, 0.51 microg kg(-1) and 1.70 microg kg(-1) for liver, 0.56 microg kg(-1) and 1.86 microg kg(-1) for muscle, respectively. Recoveries and relative standard deviation (RSD, n=9) values for precision in the developed method were from 71.14% to 88.41% and from 2.53% to 6.18%. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/20434412/Synthesis_and_characterization_of_a_molecularly_imprinted_polymer_and_its_application_as_SPE_enrichment_sorbent_for_determination_of_trace_methimazole_in_pig_samples_using_HPLC_UV_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(10)00215-1 DB - PRIME DP - Unbound Medicine ER -