Tags

Type your tag names separated by a space and hit enter

Trichomonas vaginalis: investigation of a novel diagnostic method in urine samples using cysteine proteinase 4 gene and PCR technique.
Exp Parasitol. 2010 Oct; 126(2):187-90.EP

Abstract

Trichomonas vaginalis is the agent of a highly prevalent sexually transmitted disease that leads to vaginitis, urethritis, ectocervicitis and has been associated with human immunodeficiency virus (HIV). Detection of T. vaginalis based on wet-mount microscopy and culture methods is insensitive and time consuming, respectively. Thus the quest for reliable PCR techniques of T. vaginalis in vaginal discharge and urine sample is more importance. In this study, 500 urine and vaginal-discharge samples were collected from women referred to Sexual Transmitted Disease Clinic of Mirzakuchakkhan Hospital in Tehran, Iran between May 2008 and March 2009. Wet-mount and culture methods were done on the vaginal discharges, and PCR assay targeting cysteine proteinase 4 (CP4) was performed on the urine samples. The present study demonstrated 16 (3.2%) of patients were infected with T. vaginalis using culture and wet-mount, whereas PCR assay using CP4 could detect 12 (2.4%) positivity. Sensitivity and specificity of urine PCR assay compared to culture were 80% (95% CI, 54-96) and 99.6% (95% CI, 98.96-100), respectively. These results indicate that using urine-based detection method for T. vaginalis may not be appropriate in women.

Links

Publisher Full Text

Authors+Show Affiliations

Vatanshenassan M
Medical Parasitology and Mycology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Rezaie S
No affiliation info available
Mohebali M
No affiliation info available
Niromand N
No affiliation info available
Kazemi B
No affiliation info available
Babaei Z
No affiliation info available
Rezaeian M
No affiliation info available

MeSH

AdultCysteine ProteasesFemaleHumansPolymerase Chain ReactionPredictive Value of TestsReproducibility of ResultsSensitivity and SpecificityTrichomonas VaginitisTrichomonas vaginalisUrineVagina

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20434441

Citation

Vatanshenassan, Mansoureh, et al. "Trichomonas Vaginalis: Investigation of a Novel Diagnostic Method in Urine Samples Using Cysteine Proteinase 4 Gene and PCR Technique." Experimental Parasitology, vol. 126, no. 2, 2010, pp. 187-90.
Vatanshenassan M, Rezaie S, Mohebali M, et al. Trichomonas vaginalis: investigation of a novel diagnostic method in urine samples using cysteine proteinase 4 gene and PCR technique. Exp Parasitol. 2010;126(2):187-90.
Vatanshenassan, M., Rezaie, S., Mohebali, M., Niromand, N., Kazemi, B., Babaei, Z., & Rezaeian, M. (2010). Trichomonas vaginalis: investigation of a novel diagnostic method in urine samples using cysteine proteinase 4 gene and PCR technique. Experimental Parasitology, 126(2), 187-90. https://doi.org/10.1016/j.exppara.2010.04.021
Vatanshenassan M, et al. Trichomonas Vaginalis: Investigation of a Novel Diagnostic Method in Urine Samples Using Cysteine Proteinase 4 Gene and PCR Technique. Exp Parasitol. 2010;126(2):187-90. PubMed PMID: 20434441.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Trichomonas vaginalis: investigation of a novel diagnostic method in urine samples using cysteine proteinase 4 gene and PCR technique. AU - Vatanshenassan,Mansoureh, AU - Rezaie,Sassan, AU - Mohebali,Mehdi, AU - Niromand,Nasrin, AU - Kazemi,Bahram, AU - Babaei,Zahra, AU - Rezaeian,Mostafa, Y1 - 2010/04/29/ PY - 2009/07/19/received PY - 2010/03/01/revised PY - 2010/04/22/accepted PY - 2010/5/4/entrez PY - 2010/5/4/pubmed PY - 2010/8/25/medline SP - 187 EP - 90 JF - Experimental parasitology JO - Exp Parasitol VL - 126 IS - 2 N2 - Trichomonas vaginalis is the agent of a highly prevalent sexually transmitted disease that leads to vaginitis, urethritis, ectocervicitis and has been associated with human immunodeficiency virus (HIV). Detection of T. vaginalis based on wet-mount microscopy and culture methods is insensitive and time consuming, respectively. Thus the quest for reliable PCR techniques of T. vaginalis in vaginal discharge and urine sample is more importance. In this study, 500 urine and vaginal-discharge samples were collected from women referred to Sexual Transmitted Disease Clinic of Mirzakuchakkhan Hospital in Tehran, Iran between May 2008 and March 2009. Wet-mount and culture methods were done on the vaginal discharges, and PCR assay targeting cysteine proteinase 4 (CP4) was performed on the urine samples. The present study demonstrated 16 (3.2%) of patients were infected with T. vaginalis using culture and wet-mount, whereas PCR assay using CP4 could detect 12 (2.4%) positivity. Sensitivity and specificity of urine PCR assay compared to culture were 80% (95% CI, 54-96) and 99.6% (95% CI, 98.96-100), respectively. These results indicate that using urine-based detection method for T. vaginalis may not be appropriate in women. SN - 1090-2449 UR - https://www.unboundmedicine.com/medline/citation/20434441/Trichomonas_vaginalis:_investigation_of_a_novel_diagnostic_method_in_urine_samples_using_cysteine_proteinase_4_gene_and_PCR_technique_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4894(10)00138-4 DB - PRIME DP - Unbound Medicine ER -