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Traditionally used Thai medicinal plants: in vitro anti-inflammatory, anticancer and antioxidant activities.
J Ethnopharmacol. 2010 Jul 20; 130(2):196-207.JE

Abstract

AIMS OF THE STUDY

In order to assess traditional Thai claims about the therapeutic potential of medicinal plants and to select plants for future phytochemical research, nine plant species with anti-inflammatory uses were selected from Thai textbooks and assessed for their in vitro anti-inflammatory, antiproliferative and antioxidant activities.

METHODS

Nuclear factor-kappaB (NF-kappaB) inhibitory effects in stably transfected HeLa cells were determined by luciferase assay, and effects on LPS-induced pro-inflammatory mediators prostaglandin E2 (PGE2), interleukin (IL)-6, IL-1beta, and tumour necrosis factor (TNF)alpha in primary monocytes were assessed by ELISA. Cytotoxic activities were examined against HeLa cells, human leukaemia CCRF-CEM cells and the multidrug-resistant CEM/ADR5000 subline using the MTT and XTT tests. However, a redox status has been linked with both inflammation and cancer, antioxidant effects were also assessed using the DPPH, lipid-peroxidation, and Folin-Ciocalteau methods.

RESULTS

Among all the nine species, Gynura pseudochina var. hispida and Oroxylum indicum showed the most promising NF-kappaB inhibitory effects with the lowest IC(50) values (41.96 and 47.45 microg/ml, respectively). Muehlenbeckia platyclada did not inhibit the NF-kappaB activation but effectively inhibited the release of IL-6, IL-1beta and TNF-alpha with IC(50) values ranging between 0.28 and 8.67 microg/ml. Pouzolzia indica was the most cytotoxic against CCRF-CEM cells and the multidrug-resistant CEM/ADR5000 cells (9.75% and 10.48% viability, at 10 microg/ml, respectively). Rhinacanthus nasutus was the most potent cytotoxicity against HeLa cells (IC(50) 3.63 microg/ml) and showed specific cytotoxicity against the multidrug-resistant CEM/ADR5000 cells (18.72% viability at 10 microg/ml, p<0.0001 when compared to its cytotoxicity against CCRF-CEM cells). Moreover, Oroxylum indicum showed a high level of antioxidant activity by inhibiting lipid-peroxidation (IC(50) 0.08 microg/ml).

CONCLUSIONS

This study provides in vitro evidence for the use of the Thai plants, most importantly Gynura pseudochina var. hispida, Oroxylum indicum and Muehlenbeckia platyclada as Thai anti-inflammatory remedies and these plants are now a priority for further phytochemical research.

Links

Publisher Full Text

Authors+Show Affiliations

Siriwatanametanon N
Centre for Pharmacognosy and Phytotherapy, The School of Pharmacy, University of London, London, United Kingdom.
Fiebich BL
No affiliation info available
Efferth T
No affiliation info available
Prieto JM
No affiliation info available
Heinrich M
No affiliation info available

MeSH

Anti-Inflammatory AgentsAntineoplastic Agents, PhytogenicAntioxidantsAsteraceaeBignoniaceaeCell SurvivalDinoprostoneDose-Response Relationship, DrugDrug Resistance, NeoplasmHeLa CellsHumansInflammation MediatorsInhibitory Concentration 50Interleukin-1betaInterleukin-6Lipid PeroxidationMagnoliopsidaMedicine, TraditionalMonocytesNF-kappa BPhenolsPlant ExtractsPlants, MedicinalPolygonaceaeThailandTransfectionTumor Necrosis Factor-alpha

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

20435130

Citation

Siriwatanametanon, Nisarat, et al. "Traditionally Used Thai Medicinal Plants: in Vitro Anti-inflammatory, Anticancer and Antioxidant Activities." Journal of Ethnopharmacology, vol. 130, no. 2, 2010, pp. 196-207.
Siriwatanametanon N, Fiebich BL, Efferth T, et al. Traditionally used Thai medicinal plants: in vitro anti-inflammatory, anticancer and antioxidant activities. J Ethnopharmacol. 2010;130(2):196-207.
Siriwatanametanon, N., Fiebich, B. L., Efferth, T., Prieto, J. M., & Heinrich, M. (2010). Traditionally used Thai medicinal plants: in vitro anti-inflammatory, anticancer and antioxidant activities. Journal of Ethnopharmacology, 130(2), 196-207. https://doi.org/10.1016/j.jep.2010.04.036
Siriwatanametanon N, et al. Traditionally Used Thai Medicinal Plants: in Vitro Anti-inflammatory, Anticancer and Antioxidant Activities. J Ethnopharmacol. 2010 Jul 20;130(2):196-207. PubMed PMID: 20435130.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Traditionally used Thai medicinal plants: in vitro anti-inflammatory, anticancer and antioxidant activities. AU - Siriwatanametanon,Nisarat, AU - Fiebich,Bernd L, AU - Efferth,Thomas, AU - Prieto,Jose M, AU - Heinrich,Michael, Y1 - 2010/05/08/ PY - 2009/12/03/received PY - 2010/04/15/revised PY - 2010/04/24/accepted PY - 2010/5/4/entrez PY - 2010/5/4/pubmed PY - 2010/11/5/medline SP - 196 EP - 207 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 130 IS - 2 N2 - AIMS OF THE STUDY: In order to assess traditional Thai claims about the therapeutic potential of medicinal plants and to select plants for future phytochemical research, nine plant species with anti-inflammatory uses were selected from Thai textbooks and assessed for their in vitro anti-inflammatory, antiproliferative and antioxidant activities. METHODS: Nuclear factor-kappaB (NF-kappaB) inhibitory effects in stably transfected HeLa cells were determined by luciferase assay, and effects on LPS-induced pro-inflammatory mediators prostaglandin E2 (PGE2), interleukin (IL)-6, IL-1beta, and tumour necrosis factor (TNF)alpha in primary monocytes were assessed by ELISA. Cytotoxic activities were examined against HeLa cells, human leukaemia CCRF-CEM cells and the multidrug-resistant CEM/ADR5000 subline using the MTT and XTT tests. However, a redox status has been linked with both inflammation and cancer, antioxidant effects were also assessed using the DPPH, lipid-peroxidation, and Folin-Ciocalteau methods. RESULTS: Among all the nine species, Gynura pseudochina var. hispida and Oroxylum indicum showed the most promising NF-kappaB inhibitory effects with the lowest IC(50) values (41.96 and 47.45 microg/ml, respectively). Muehlenbeckia platyclada did not inhibit the NF-kappaB activation but effectively inhibited the release of IL-6, IL-1beta and TNF-alpha with IC(50) values ranging between 0.28 and 8.67 microg/ml. Pouzolzia indica was the most cytotoxic against CCRF-CEM cells and the multidrug-resistant CEM/ADR5000 cells (9.75% and 10.48% viability, at 10 microg/ml, respectively). Rhinacanthus nasutus was the most potent cytotoxicity against HeLa cells (IC(50) 3.63 microg/ml) and showed specific cytotoxicity against the multidrug-resistant CEM/ADR5000 cells (18.72% viability at 10 microg/ml, p<0.0001 when compared to its cytotoxicity against CCRF-CEM cells). Moreover, Oroxylum indicum showed a high level of antioxidant activity by inhibiting lipid-peroxidation (IC(50) 0.08 microg/ml). CONCLUSIONS: This study provides in vitro evidence for the use of the Thai plants, most importantly Gynura pseudochina var. hispida, Oroxylum indicum and Muehlenbeckia platyclada as Thai anti-inflammatory remedies and these plants are now a priority for further phytochemical research. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/20435130/Traditionally_used_Thai_medicinal_plants:_in_vitro_anti_inflammatory_anticancer_and_antioxidant_activities_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(10)00268-0 DB - PRIME DP - Unbound Medicine ER -