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C-type natriuretic peptide modulates bidirectional plasticity in hippocampal area CA1 in vitro.
Neuroscience. 2010 Aug 11; 169(1):8-22.N

Abstract

C-type natriuretic peptide (CNP) and the natriuretic peptide receptor B (NPR-B) are expressed throughout the hippocampus. We tested whether CNP affected long-term potentiation (LTP) or long-term depression (LTD) in area CA1. Field potentials (FP) were simultaneously recorded in stratum pyramidale (SP) and stratum radiatum (SR) of area CA1 in rat hippocampal slices. To induce LTD and LTP stimulation was applied to SR in area CA1 at 1 and 5 Hz and 30-100 Hz, respectively. CNP (100 nM) increased LTD magnitude while LTP induction was impeded. Thus, in the presence of CNP the threshold for LTP induction was shifted to higher stimulus frequencies, a modulation that showed layer-specific differences in area CA1. Effects of CNP were prevented by the NPR-B antagonist HS-142-1. In the presence of the GABA(A) receptor blocker bicuculline (BMI, 5 microM), CNP-mediated effects were attenuated in SP and SR. Intracellular recordings under this condition revealed that CNP significantly reduced number of action potentials generated during depolarizing current steps. The input resistance of CA1 cells and amplitude of isolated excitatory postsynaptic potential (EPSPs) were significantly increased by CNP whereas these changes were not observed in the absence of BMI. 100 Hz stimulation induced stable potentiation of the EPSP amplitude in CA1 pyramidal cells while this effect was strongly attenuated by CNP. This effect was prevented by BMI. Immunohistochemistry indicated that the peptide binds to receptors expressed on pyramidal cells and GAD(65/67)-immunopositive interneurons. 20 Hz stimulation, applied for 30 s, induced LTP in SR and SP. CNP attenuated LTP in SP and reversed LTP into LTD in SR. These effects were mimicked by low-dose dl-2-amino-5-phosphonopentanoic acid (dl-APV) (10 microM) suggesting partial N-methyl d-aspartate (NMDA) receptor dependency of CNP-mediated effects. Together, our data suggest that CNP is involved in the regulation of bidirectional plasticity in area CA1 potentially by modulating GABA(A)-mediated inhibition and NMDA receptors.

Authors+Show Affiliations

Institute of Neurophysiology, Johannes Müller Center of Physiology, Charite - Universitätsmedizin Berlin, Tucholsky Strasse 2, 10117 Berlin, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20438814

Citation

Decker, J M., et al. "C-type Natriuretic Peptide Modulates Bidirectional Plasticity in Hippocampal Area CA1 in Vitro." Neuroscience, vol. 169, no. 1, 2010, pp. 8-22.
Decker JM, Wójtowicz AM, Bartsch JC, et al. C-type natriuretic peptide modulates bidirectional plasticity in hippocampal area CA1 in vitro. Neuroscience. 2010;169(1):8-22.
Decker, J. M., Wójtowicz, A. M., Bartsch, J. C., Liotta, A., Braunewell, K. H., Heinemann, U., & Behrens, C. J. (2010). C-type natriuretic peptide modulates bidirectional plasticity in hippocampal area CA1 in vitro. Neuroscience, 169(1), 8-22. https://doi.org/10.1016/j.neuroscience.2010.04.064
Decker JM, et al. C-type Natriuretic Peptide Modulates Bidirectional Plasticity in Hippocampal Area CA1 in Vitro. Neuroscience. 2010 Aug 11;169(1):8-22. PubMed PMID: 20438814.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - C-type natriuretic peptide modulates bidirectional plasticity in hippocampal area CA1 in vitro. AU - Decker,J M, AU - Wójtowicz,A M, AU - Bartsch,J C, AU - Liotta,A, AU - Braunewell,K H, AU - Heinemann,U, AU - Behrens,C J, Y1 - 2010/05/08/ PY - 2009/12/07/received PY - 2010/03/29/revised PY - 2010/04/26/accepted PY - 2010/5/5/entrez PY - 2010/5/5/pubmed PY - 2011/3/5/medline SP - 8 EP - 22 JF - Neuroscience JO - Neuroscience VL - 169 IS - 1 N2 - C-type natriuretic peptide (CNP) and the natriuretic peptide receptor B (NPR-B) are expressed throughout the hippocampus. We tested whether CNP affected long-term potentiation (LTP) or long-term depression (LTD) in area CA1. Field potentials (FP) were simultaneously recorded in stratum pyramidale (SP) and stratum radiatum (SR) of area CA1 in rat hippocampal slices. To induce LTD and LTP stimulation was applied to SR in area CA1 at 1 and 5 Hz and 30-100 Hz, respectively. CNP (100 nM) increased LTD magnitude while LTP induction was impeded. Thus, in the presence of CNP the threshold for LTP induction was shifted to higher stimulus frequencies, a modulation that showed layer-specific differences in area CA1. Effects of CNP were prevented by the NPR-B antagonist HS-142-1. In the presence of the GABA(A) receptor blocker bicuculline (BMI, 5 microM), CNP-mediated effects were attenuated in SP and SR. Intracellular recordings under this condition revealed that CNP significantly reduced number of action potentials generated during depolarizing current steps. The input resistance of CA1 cells and amplitude of isolated excitatory postsynaptic potential (EPSPs) were significantly increased by CNP whereas these changes were not observed in the absence of BMI. 100 Hz stimulation induced stable potentiation of the EPSP amplitude in CA1 pyramidal cells while this effect was strongly attenuated by CNP. This effect was prevented by BMI. Immunohistochemistry indicated that the peptide binds to receptors expressed on pyramidal cells and GAD(65/67)-immunopositive interneurons. 20 Hz stimulation, applied for 30 s, induced LTP in SR and SP. CNP attenuated LTP in SP and reversed LTP into LTD in SR. These effects were mimicked by low-dose dl-2-amino-5-phosphonopentanoic acid (dl-APV) (10 microM) suggesting partial N-methyl d-aspartate (NMDA) receptor dependency of CNP-mediated effects. Together, our data suggest that CNP is involved in the regulation of bidirectional plasticity in area CA1 potentially by modulating GABA(A)-mediated inhibition and NMDA receptors. SN - 1873-7544 UR - https://www.unboundmedicine.com/medline/citation/20438814/C_type_natriuretic_peptide_modulates_bidirectional_plasticity_in_hippocampal_area_CA1_in_vitro_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306-4522(10)00631-7 DB - PRIME DP - Unbound Medicine ER -