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Dryopteris crassirhizoma has anti-cancer effects through both extrinsic and intrinsic apoptotic pathways and G0/G1 phase arrest in human prostate cancer cells.
J Ethnopharmacol. 2010 Jul 20; 130(2):248-54.JE

Abstract

AIM OF THE STUDY

The inhibitory effect of Dryopteris crassirhizoma on the proliferation of human metastatic prostate PC3-MM2 cells and the mechanism of action were examined to identify its anti-cancer properties. The effect of the extract on cell cycle progression and its combined cytotoxic effect with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) on PC3-MM2 cells were also investigated.

MATERIALS AND METHODS

The anti-proliferative effects of Dryopteris crassirhizoma were examined by culturing PC3-MM2 cells in the presence or absence of various concentrations of Dryopteris crassirhizoma extract, and the inhibitory effects on cell proliferation were determined by Cell Counting Kit (CCK)-8 analysis. The quantities of apoptosis-inducing proteins were measured by western blotting analysis. Cell cycle progression was analyzed by PI staining using flow cytometry.

RESULTS

Dryopteris crassirhizoma (50 and 100 microg/ml) inhibited markedly the proliferation of PC-3 and PC3-MM2 cells without cytotoxicity to normal (spleen) cells from BALB/C mice. Dryopteris crassirhizoma (100 microg/ml) effectively induced apoptosis through the activation of caspase-3, -8, -9, bid, and PARP in PC3-MM2 cells. The cells exposed to Dryopteris crassirhizoma increased significantly the accumulation of the DNA contents in the G0/G1 phase and sub-G1 phase in contrast to the control. The combined cytotoxic effects of Dryopteris crassirhizoma and TRAIL induced the increased activity of 29% in contrast to the sum of the inhibitory effects of each agent alone.

CONCLUSIONS

Dryopteris crassirhizoma has anti-cancer properties by inducing cell cycle arrest and apoptosis through the extrinsic and intrinsic pathway in PC3-MM2 cells. The extract also showed a combined effect with TRAIL on the inhibition of proliferation in the cells. These findings suggest that possibly its extract could be used for treating androgen-independent prostate cancer with minimal side effects.

Authors+Show Affiliations

BK21 Medical Science Education Center, Department of Biochemistry, Pusan National University School of Medicine, Yangsan-City, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20438825

Citation

Chang, Sung-Ho, et al. "Dryopteris Crassirhizoma Has Anti-cancer Effects Through Both Extrinsic and Intrinsic Apoptotic Pathways and G0/G1 Phase Arrest in Human Prostate Cancer Cells." Journal of Ethnopharmacology, vol. 130, no. 2, 2010, pp. 248-54.
Chang SH, Bae JH, Hong DP, et al. Dryopteris crassirhizoma has anti-cancer effects through both extrinsic and intrinsic apoptotic pathways and G0/G1 phase arrest in human prostate cancer cells. J Ethnopharmacol. 2010;130(2):248-54.
Chang, S. H., Bae, J. H., Hong, D. P., Choi, K. D., Kim, S. C., Her, E., Kim, S. H., & Kang, C. D. (2010). Dryopteris crassirhizoma has anti-cancer effects through both extrinsic and intrinsic apoptotic pathways and G0/G1 phase arrest in human prostate cancer cells. Journal of Ethnopharmacology, 130(2), 248-54. https://doi.org/10.1016/j.jep.2010.04.038
Chang SH, et al. Dryopteris Crassirhizoma Has Anti-cancer Effects Through Both Extrinsic and Intrinsic Apoptotic Pathways and G0/G1 Phase Arrest in Human Prostate Cancer Cells. J Ethnopharmacol. 2010 Jul 20;130(2):248-54. PubMed PMID: 20438825.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dryopteris crassirhizoma has anti-cancer effects through both extrinsic and intrinsic apoptotic pathways and G0/G1 phase arrest in human prostate cancer cells. AU - Chang,Sung-Ho, AU - Bae,Jae-Ho, AU - Hong,Doo-Pyo, AU - Choi,Kang-Duk, AU - Kim,Song-Cheol, AU - Her,Erk, AU - Kim,Sun-Hee, AU - Kang,Chi-Dug, Y1 - 2010/05/08/ PY - 2009/12/11/received PY - 2010/04/13/revised PY - 2010/04/24/accepted PY - 2010/5/5/entrez PY - 2010/5/5/pubmed PY - 2010/11/5/medline SP - 248 EP - 54 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 130 IS - 2 N2 - AIM OF THE STUDY: The inhibitory effect of Dryopteris crassirhizoma on the proliferation of human metastatic prostate PC3-MM2 cells and the mechanism of action were examined to identify its anti-cancer properties. The effect of the extract on cell cycle progression and its combined cytotoxic effect with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) on PC3-MM2 cells were also investigated. MATERIALS AND METHODS: The anti-proliferative effects of Dryopteris crassirhizoma were examined by culturing PC3-MM2 cells in the presence or absence of various concentrations of Dryopteris crassirhizoma extract, and the inhibitory effects on cell proliferation were determined by Cell Counting Kit (CCK)-8 analysis. The quantities of apoptosis-inducing proteins were measured by western blotting analysis. Cell cycle progression was analyzed by PI staining using flow cytometry. RESULTS: Dryopteris crassirhizoma (50 and 100 microg/ml) inhibited markedly the proliferation of PC-3 and PC3-MM2 cells without cytotoxicity to normal (spleen) cells from BALB/C mice. Dryopteris crassirhizoma (100 microg/ml) effectively induced apoptosis through the activation of caspase-3, -8, -9, bid, and PARP in PC3-MM2 cells. The cells exposed to Dryopteris crassirhizoma increased significantly the accumulation of the DNA contents in the G0/G1 phase and sub-G1 phase in contrast to the control. The combined cytotoxic effects of Dryopteris crassirhizoma and TRAIL induced the increased activity of 29% in contrast to the sum of the inhibitory effects of each agent alone. CONCLUSIONS: Dryopteris crassirhizoma has anti-cancer properties by inducing cell cycle arrest and apoptosis through the extrinsic and intrinsic pathway in PC3-MM2 cells. The extract also showed a combined effect with TRAIL on the inhibition of proliferation in the cells. These findings suggest that possibly its extract could be used for treating androgen-independent prostate cancer with minimal side effects. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/20438825/Dryopteris_crassirhizoma_has_anti_cancer_effects_through_both_extrinsic_and_intrinsic_apoptotic_pathways_and_G0/G1_phase_arrest_in_human_prostate_cancer_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(10)00270-9 DB - PRIME DP - Unbound Medicine ER -