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Carbon nanotube-enhanced electrochemical aptasensor for the detection of thrombin.
Talanta. 2010 Jun 15; 81(4-5):1619-24.T

Abstract

A novel electrochemical aptasensor for the detection of thrombin was developed on basis of the thrombin-binding aptamer (TBA) as a molecular recognition element and multi-walled carbon nanotubes (MWCNTs) as a carrier of the electrochemical capture probe. Amine-modified capture probe (12-mer) was covalently conjugated to the MWCNTs modified glassy carbon electrode (GCE). The target aptamer probe (21-mer) contains TBA (15-mer) labeled with ferrocene (Fc), which is designed to hybridize with capture probe and specifically recognize thrombin, is immobilized on the electrode surface by hybridization reaction. Introduction of the analyte thrombin triggered the dissociation of the aptamer probe labeled with Fc from the biosensors, led to a significant decrease in peak current intensity. Differential pulse voltammetry (DPV) was employed to detect the target analyte with different concentrations. The decreased peak current was in proportion to the concentration of thrombin in a range from 1.0x10(-12) to 5.0x10(-10)M with a detection limit of 5x10(-13)M. The present work demonstrates that using MWCNTs as a carrier for electrochemical capture probe is a promising way to amplify the electrochemical signal and to improve the sensitivity of the electrochemical aptasensor.

Authors+Show Affiliations

Institute of Analytical Science, Northwest University, Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Xi'an, Shaanxi, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20441948

Citation

Liu, Xiaorong, et al. "Carbon Nanotube-enhanced Electrochemical Aptasensor for the Detection of Thrombin." Talanta, vol. 81, no. 4-5, 2010, pp. 1619-24.
Liu X, Li Y, Zheng J, et al. Carbon nanotube-enhanced electrochemical aptasensor for the detection of thrombin. Talanta. 2010;81(4-5):1619-24.
Liu, X., Li, Y., Zheng, J., Zhang, J., & Sheng, Q. (2010). Carbon nanotube-enhanced electrochemical aptasensor for the detection of thrombin. Talanta, 81(4-5), 1619-24. https://doi.org/10.1016/j.talanta.2010.03.013
Liu X, et al. Carbon Nanotube-enhanced Electrochemical Aptasensor for the Detection of Thrombin. Talanta. 2010 Jun 15;81(4-5):1619-24. PubMed PMID: 20441948.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Carbon nanotube-enhanced electrochemical aptasensor for the detection of thrombin. AU - Liu,Xiaorong, AU - Li,Yan, AU - Zheng,Jianbin, AU - Zhang,Juncai, AU - Sheng,Qinglin, Y1 - 2010/03/19/ PY - 2009/11/19/received PY - 2010/03/03/revised PY - 2010/03/09/accepted PY - 2010/5/6/entrez PY - 2010/5/6/pubmed PY - 2010/10/22/medline SP - 1619 EP - 24 JF - Talanta JO - Talanta VL - 81 IS - 4-5 N2 - A novel electrochemical aptasensor for the detection of thrombin was developed on basis of the thrombin-binding aptamer (TBA) as a molecular recognition element and multi-walled carbon nanotubes (MWCNTs) as a carrier of the electrochemical capture probe. Amine-modified capture probe (12-mer) was covalently conjugated to the MWCNTs modified glassy carbon electrode (GCE). The target aptamer probe (21-mer) contains TBA (15-mer) labeled with ferrocene (Fc), which is designed to hybridize with capture probe and specifically recognize thrombin, is immobilized on the electrode surface by hybridization reaction. Introduction of the analyte thrombin triggered the dissociation of the aptamer probe labeled with Fc from the biosensors, led to a significant decrease in peak current intensity. Differential pulse voltammetry (DPV) was employed to detect the target analyte with different concentrations. The decreased peak current was in proportion to the concentration of thrombin in a range from 1.0x10(-12) to 5.0x10(-10)M with a detection limit of 5x10(-13)M. The present work demonstrates that using MWCNTs as a carrier for electrochemical capture probe is a promising way to amplify the electrochemical signal and to improve the sensitivity of the electrochemical aptasensor. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/20441948/Carbon_nanotube_enhanced_electrochemical_aptasensor_for_the_detection_of_thrombin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(10)00207-9 DB - PRIME DP - Unbound Medicine ER -