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Ultra-sensitive detection of IgE using biofunctionalized nanoparticle-enhanced SPR.
Talanta. 2010 Jun 15; 81(4-5):1755-9.T

Abstract

This paper describes an ultra-sensitive surface-based detection method using nanoparticle-enhanced surface plasmon resonance (SPR) for the detection of immunoglobulin E (IgE) proteins, which could potentially be used for the diagnosis of allergic diseases. Two different probes, anti-IgE and IgE specific aptamers, which can specifically interact with IgE at different epitopes were first investigated for their specific interaction with IgE using SPR. Langmuir adsorption coefficient (K(ads)) values were measured as 2.0(+/-0.22)x10(8)M(-1) and 2.2(+/-0.20)x10(8)M(-1) for IgE interactions with anti-IgE and IgE specific aptamers, respectively. The SPR detection limit of the simple adsorption of IgE onto either anti-IgE or IgE specific aptamers was found to be about 1nM. In order to improve the SPR detection signal for IgE, two different approaches utilizing surface formed sandwich complexes with biofunctionalized gold nanoparticles (Au-Nps) were designed and their detection performance were compared; the complexes were created via the adsorption of IgE onto (i) surface immobilized anti-IgE followed by the adsorption of IgE specific aptamer coated gold nanoparticles and (ii) IgE specific aptamer surface with the subsequent adsorption of anti-IgE coated gold nanoparticles. Both detection schemes were able to directly measure IgE at femtomolar concentrations.

Authors+Show Affiliations

Department of Chemistry, Kyungpook National University, Daegu, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20441969

Citation

Kim, Suhee, et al. "Ultra-sensitive Detection of IgE Using Biofunctionalized Nanoparticle-enhanced SPR." Talanta, vol. 81, no. 4-5, 2010, pp. 1755-9.
Kim S, Lee J, Lee SJ, et al. Ultra-sensitive detection of IgE using biofunctionalized nanoparticle-enhanced SPR. Talanta. 2010;81(4-5):1755-9.
Kim, S., Lee, J., Lee, S. J., & Lee, H. J. (2010). Ultra-sensitive detection of IgE using biofunctionalized nanoparticle-enhanced SPR. Talanta, 81(4-5), 1755-9. https://doi.org/10.1016/j.talanta.2010.03.036
Kim S, et al. Ultra-sensitive Detection of IgE Using Biofunctionalized Nanoparticle-enhanced SPR. Talanta. 2010 Jun 15;81(4-5):1755-9. PubMed PMID: 20441969.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultra-sensitive detection of IgE using biofunctionalized nanoparticle-enhanced SPR. AU - Kim,Suhee, AU - Lee,Jaeyoung, AU - Lee,Seok Jae, AU - Lee,Hye Jin, Y1 - 2010/03/25/ PY - 2010/01/21/received PY - 2010/03/18/revised PY - 2010/03/18/accepted PY - 2010/5/6/entrez PY - 2010/5/6/pubmed PY - 2010/10/22/medline SP - 1755 EP - 9 JF - Talanta JO - Talanta VL - 81 IS - 4-5 N2 - This paper describes an ultra-sensitive surface-based detection method using nanoparticle-enhanced surface plasmon resonance (SPR) for the detection of immunoglobulin E (IgE) proteins, which could potentially be used for the diagnosis of allergic diseases. Two different probes, anti-IgE and IgE specific aptamers, which can specifically interact with IgE at different epitopes were first investigated for their specific interaction with IgE using SPR. Langmuir adsorption coefficient (K(ads)) values were measured as 2.0(+/-0.22)x10(8)M(-1) and 2.2(+/-0.20)x10(8)M(-1) for IgE interactions with anti-IgE and IgE specific aptamers, respectively. The SPR detection limit of the simple adsorption of IgE onto either anti-IgE or IgE specific aptamers was found to be about 1nM. In order to improve the SPR detection signal for IgE, two different approaches utilizing surface formed sandwich complexes with biofunctionalized gold nanoparticles (Au-Nps) were designed and their detection performance were compared; the complexes were created via the adsorption of IgE onto (i) surface immobilized anti-IgE followed by the adsorption of IgE specific aptamer coated gold nanoparticles and (ii) IgE specific aptamer surface with the subsequent adsorption of anti-IgE coated gold nanoparticles. Both detection schemes were able to directly measure IgE at femtomolar concentrations. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/20441969/Ultra_sensitive_detection_of_IgE_using_biofunctionalized_nanoparticle_enhanced_SPR_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(10)00230-4 DB - PRIME DP - Unbound Medicine ER -