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Inhibition of three selected beverage extracts on alpha-glucosidase and rapid identification of their active compounds using HPLC-DAD-MS/MS and biochemical detection.
J Agric Food Chem. 2010 Jun 09; 58(11):6608-13.JA

Abstract

Inhibition of alpha-glucosidase is a therapeutic approach for diabetes. In this study, a method based on online liquid chromatography-diode array detection-tandem mass spectrometry and biochemical detection (LC-DAD-MS/MS-BCD) was developed to screen and identify alpha-glucosidase inhibitors from selected beverage extracts, including pu-erh tea (Camellia sinensis var. assamica), eagle tea (Litsea coreana Levl.), and radix glycyrrhizae (Glycyrrhiza uralensis Fisch.). As a result, two components, (-)-epigallocatechingallate (EGCG) and (-)-epicatechingallate (ECG), as potent alpha-glucosidase inhibitors, were found in pu-erh tea. The IC(50) values of EGCG and ECG on alpha-glucosidase (EC 3.2.1.20, from Saccharomyces cerevisiae) were 175.1 and 246.9 microM, respectively, and both were lower than that of acarbose (IC(50) = 3553.0 microM), a commercial alpha-glucosidase inhibitor. Kinetic studies revealed that both EGCG and ECG inhibited alpha-glucosidase activity in a noncompetitive manner. The study suggests that the developed LC-DAD-MS/MS-BCD system is a powerful tool for rapid screening and identification of alpha-glucosidase inhibitors in complex samples and that EGCG and ECG may be good candidates as alpha-glucosidase inhibitors.

Authors+Show Affiliations

Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20443596

Citation

Li, De-Qiang, et al. "Inhibition of Three Selected Beverage Extracts On Alpha-glucosidase and Rapid Identification of Their Active Compounds Using HPLC-DAD-MS/MS and Biochemical Detection." Journal of Agricultural and Food Chemistry, vol. 58, no. 11, 2010, pp. 6608-13.
Li DQ, Qian ZM, Li SP. Inhibition of three selected beverage extracts on alpha-glucosidase and rapid identification of their active compounds using HPLC-DAD-MS/MS and biochemical detection. J Agric Food Chem. 2010;58(11):6608-13.
Li, D. Q., Qian, Z. M., & Li, S. P. (2010). Inhibition of three selected beverage extracts on alpha-glucosidase and rapid identification of their active compounds using HPLC-DAD-MS/MS and biochemical detection. Journal of Agricultural and Food Chemistry, 58(11), 6608-13. https://doi.org/10.1021/jf100853c
Li DQ, Qian ZM, Li SP. Inhibition of Three Selected Beverage Extracts On Alpha-glucosidase and Rapid Identification of Their Active Compounds Using HPLC-DAD-MS/MS and Biochemical Detection. J Agric Food Chem. 2010 Jun 9;58(11):6608-13. PubMed PMID: 20443596.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of three selected beverage extracts on alpha-glucosidase and rapid identification of their active compounds using HPLC-DAD-MS/MS and biochemical detection. AU - Li,De-Qiang, AU - Qian,Zheng-Ming, AU - Li,Shao-Ping, PY - 2010/5/7/entrez PY - 2010/5/7/pubmed PY - 2010/8/28/medline SP - 6608 EP - 13 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 58 IS - 11 N2 - Inhibition of alpha-glucosidase is a therapeutic approach for diabetes. In this study, a method based on online liquid chromatography-diode array detection-tandem mass spectrometry and biochemical detection (LC-DAD-MS/MS-BCD) was developed to screen and identify alpha-glucosidase inhibitors from selected beverage extracts, including pu-erh tea (Camellia sinensis var. assamica), eagle tea (Litsea coreana Levl.), and radix glycyrrhizae (Glycyrrhiza uralensis Fisch.). As a result, two components, (-)-epigallocatechingallate (EGCG) and (-)-epicatechingallate (ECG), as potent alpha-glucosidase inhibitors, were found in pu-erh tea. The IC(50) values of EGCG and ECG on alpha-glucosidase (EC 3.2.1.20, from Saccharomyces cerevisiae) were 175.1 and 246.9 microM, respectively, and both were lower than that of acarbose (IC(50) = 3553.0 microM), a commercial alpha-glucosidase inhibitor. Kinetic studies revealed that both EGCG and ECG inhibited alpha-glucosidase activity in a noncompetitive manner. The study suggests that the developed LC-DAD-MS/MS-BCD system is a powerful tool for rapid screening and identification of alpha-glucosidase inhibitors in complex samples and that EGCG and ECG may be good candidates as alpha-glucosidase inhibitors. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/20443596/Inhibition_of_three_selected_beverage_extracts_on_alpha_glucosidase_and_rapid_identification_of_their_active_compounds_using_HPLC_DAD_MS/MS_and_biochemical_detection_ L2 - https://doi.org/10.1021/jf100853c DB - PRIME DP - Unbound Medicine ER -