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Simultaneous determination of memantine and amantadine in human plasma as fluorescein derivatives by micellar electrokinetic chromatography with laser-induced fluorescence detection and its clinical application.
Electrophoresis. 2010 Jun; 31(11):1903-11.E

Abstract

A nonionic surfactant MEKC method with LIF detection was developed for the simultaneous determination of memantine, an anti-Alzheimer's disease agent, and amantadine, an anti-Parkinson's disease drug, in human plasma. Before analysis, the plasma samples were pretreated by liquid-liquid extraction with ethyl acetate, and derivatized with 6-carboxyfluorescein N-hydroxysuccinimide ester. The chemical derivatization is performed with 6-carboxyfluorescein N-hydroxysuccinimide ester in ACN - 5 mM pH 9.0 borate buffer (40:60, v/v) at 35 degrees C for 3 h. After the derivatization reaction, hydrodynamic injection (0.5 psi, 8 s) was used to introduce the derivatized solution, and the separation was performed in borate buffer (30 mM, pH 9.5) with the nonionic surfactant Brij-35 (0.07%, w/v); the separation voltage was 6 kV. The linear ranges of the method for the determination of memantine and amantadine in human plasma were over a range of 2.0-60.0 ng/mL. The detection limit was 0.5 ng/mL (S/N=3). This method was applied successfully to monitor the concentration of memantine or amantadine in patients with Alzheimer's disease or Parkinson's disease.

Authors+Show Affiliations

School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20446294

Citation

Yeh, Hsin-Hua, et al. "Simultaneous Determination of Memantine and Amantadine in Human Plasma as Fluorescein Derivatives By Micellar Electrokinetic Chromatography With Laser-induced Fluorescence Detection and Its Clinical Application." Electrophoresis, vol. 31, no. 11, 2010, pp. 1903-11.
Yeh HH, Yang YH, Chen SH. Simultaneous determination of memantine and amantadine in human plasma as fluorescein derivatives by micellar electrokinetic chromatography with laser-induced fluorescence detection and its clinical application. Electrophoresis. 2010;31(11):1903-11.
Yeh, H. H., Yang, Y. H., & Chen, S. H. (2010). Simultaneous determination of memantine and amantadine in human plasma as fluorescein derivatives by micellar electrokinetic chromatography with laser-induced fluorescence detection and its clinical application. Electrophoresis, 31(11), 1903-11. https://doi.org/10.1002/elps.201000001
Yeh HH, Yang YH, Chen SH. Simultaneous Determination of Memantine and Amantadine in Human Plasma as Fluorescein Derivatives By Micellar Electrokinetic Chromatography With Laser-induced Fluorescence Detection and Its Clinical Application. Electrophoresis. 2010;31(11):1903-11. PubMed PMID: 20446294.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of memantine and amantadine in human plasma as fluorescein derivatives by micellar electrokinetic chromatography with laser-induced fluorescence detection and its clinical application. AU - Yeh,Hsin-Hua, AU - Yang,Yuan-Han, AU - Chen,Su-Hwei, PY - 2010/5/7/entrez PY - 2010/5/7/pubmed PY - 2010/8/25/medline SP - 1903 EP - 11 JF - Electrophoresis JO - Electrophoresis VL - 31 IS - 11 N2 - A nonionic surfactant MEKC method with LIF detection was developed for the simultaneous determination of memantine, an anti-Alzheimer's disease agent, and amantadine, an anti-Parkinson's disease drug, in human plasma. Before analysis, the plasma samples were pretreated by liquid-liquid extraction with ethyl acetate, and derivatized with 6-carboxyfluorescein N-hydroxysuccinimide ester. The chemical derivatization is performed with 6-carboxyfluorescein N-hydroxysuccinimide ester in ACN - 5 mM pH 9.0 borate buffer (40:60, v/v) at 35 degrees C for 3 h. After the derivatization reaction, hydrodynamic injection (0.5 psi, 8 s) was used to introduce the derivatized solution, and the separation was performed in borate buffer (30 mM, pH 9.5) with the nonionic surfactant Brij-35 (0.07%, w/v); the separation voltage was 6 kV. The linear ranges of the method for the determination of memantine and amantadine in human plasma were over a range of 2.0-60.0 ng/mL. The detection limit was 0.5 ng/mL (S/N=3). This method was applied successfully to monitor the concentration of memantine or amantadine in patients with Alzheimer's disease or Parkinson's disease. SN - 1522-2683 UR - https://www.unboundmedicine.com/medline/citation/20446294/Simultaneous_determination_of_memantine_and_amantadine_in_human_plasma_as_fluorescein_derivatives_by_micellar_electrokinetic_chromatography_with_laser_induced_fluorescence_detection_and_its_clinical_application_ L2 - https://doi.org/10.1002/elps.201000001 DB - PRIME DP - Unbound Medicine ER -