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Establishment of L-arabinose fermentation in glucose/xylose co-fermenting recombinant Saccharomyces cerevisiae 424A(LNH-ST) by genetic engineering.
Appl Microbiol Biotechnol. 2010 Aug; 87(5):1803-11.AM

Abstract

Cost-effective and efficient ethanol production from lignocellulosic materials requires the fermentation of all sugars recovered from such materials including glucose, xylose, mannose, galactose, and L-arabinose. Wild-type strains of Saccharomyces cerevisiae used in industrial ethanol production cannot ferment D-xylose and L-arabinose. Our genetically engineered recombinant S. cerevisiae yeast 424A(LNH-ST) has been made able to efficiently ferment xylose to ethanol, which was achieved by integrating multiple copies of three xylose-metabolizing genes. This study reports the efficient anaerobic fermentation of L-arabinose by the derivative of 424A(LNH-ST). The new strain was constructed by over-expression of two additional genes from fungi L-arabinose utilization pathways. The resulting new 424A(LNH-ST) strain exhibited production of ethanol from L-arabinose, and the yield was more than 40%. An efficient ethanol production, about 72.5% yield from five-sugar mixtures containing glucose, galactose, mannose, xylose, and arabinose was also achieved. This co-fermentation of five-sugar mixture is important and crucial for application in industrial economical ethanol production using lignocellulosic biomass as the feedstock.

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Authors+Show Affiliations

Bera AK
Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN, USA.
Sedlak M
No affiliation info available
Khan A
No affiliation info available
Ho NW
No affiliation info available

MeSH

ArabinoseEthanolFermentationGenetic EngineeringGlucoseLigninMetabolic Networks and PathwaysRecombinant ProteinsSaccharomyces cerevisiaeXylose

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

20449743

Citation

Bera, Aloke Kumar, et al. "Establishment of L-arabinose Fermentation in Glucose/xylose Co-fermenting Recombinant Saccharomyces Cerevisiae 424A(LNH-ST) By Genetic Engineering." Applied Microbiology and Biotechnology, vol. 87, no. 5, 2010, pp. 1803-11.
Bera AK, Sedlak M, Khan A, et al. Establishment of L-arabinose fermentation in glucose/xylose co-fermenting recombinant Saccharomyces cerevisiae 424A(LNH-ST) by genetic engineering. Appl Microbiol Biotechnol. 2010;87(5):1803-11.
Bera, A. K., Sedlak, M., Khan, A., & Ho, N. W. (2010). Establishment of L-arabinose fermentation in glucose/xylose co-fermenting recombinant Saccharomyces cerevisiae 424A(LNH-ST) by genetic engineering. Applied Microbiology and Biotechnology, 87(5), 1803-11. https://doi.org/10.1007/s00253-010-2609-0
Bera AK, et al. Establishment of L-arabinose Fermentation in Glucose/xylose Co-fermenting Recombinant Saccharomyces Cerevisiae 424A(LNH-ST) By Genetic Engineering. Appl Microbiol Biotechnol. 2010;87(5):1803-11. PubMed PMID: 20449743.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Establishment of L-arabinose fermentation in glucose/xylose co-fermenting recombinant Saccharomyces cerevisiae 424A(LNH-ST) by genetic engineering. AU - Bera,Aloke Kumar, AU - Sedlak,Miroslav, AU - Khan,Aftab, AU - Ho,Nancy W Y, Y1 - 2010/05/07/ PY - 2010/01/11/received PY - 2010/04/09/accepted PY - 2010/04/07/revised PY - 2010/5/8/entrez PY - 2010/5/8/pubmed PY - 2010/10/15/medline SP - 1803 EP - 11 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 87 IS - 5 N2 - Cost-effective and efficient ethanol production from lignocellulosic materials requires the fermentation of all sugars recovered from such materials including glucose, xylose, mannose, galactose, and L-arabinose. Wild-type strains of Saccharomyces cerevisiae used in industrial ethanol production cannot ferment D-xylose and L-arabinose. Our genetically engineered recombinant S. cerevisiae yeast 424A(LNH-ST) has been made able to efficiently ferment xylose to ethanol, which was achieved by integrating multiple copies of three xylose-metabolizing genes. This study reports the efficient anaerobic fermentation of L-arabinose by the derivative of 424A(LNH-ST). The new strain was constructed by over-expression of two additional genes from fungi L-arabinose utilization pathways. The resulting new 424A(LNH-ST) strain exhibited production of ethanol from L-arabinose, and the yield was more than 40%. An efficient ethanol production, about 72.5% yield from five-sugar mixtures containing glucose, galactose, mannose, xylose, and arabinose was also achieved. This co-fermentation of five-sugar mixture is important and crucial for application in industrial economical ethanol production using lignocellulosic biomass as the feedstock. SN - 1432-0614 UR - https://www.unboundmedicine.com/medline/citation/20449743/Establishment_of_L_arabinose_fermentation_in_glucose/xylose_co_fermenting_recombinant_Saccharomyces_cerevisiae_424A_LNH_ST__by_genetic_engineering_ L2 - https://dx.doi.org/10.1007/s00253-010-2609-0 DB - PRIME DP - Unbound Medicine ER -