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[Involvement of JNK and caspase-3 in human lymphoma cell apoptosis induced by Prunella vulgaris].
Zhonghua Yi Xue Za Zhi. 2010 Mar 16; 90(10):690-3.ZY

Abstract

OBJECTIVE

To characterize the effect of Prunella vulgaris upon lymphoma cells and its possible mechanisms.

METHODS

Raji cells were subjected to different therapeutic schemes: Prunella vulgaris (50 g/L) group, P. vulgaris (50 g/L)+SP600125 (20 micromol/L, JNK inhibitor) (SP600125 group); Raji cells in physiological saline served as the control. MTT assay was used to measure the cellular proliferation. Phosphorylation of JNK, c-Jun and the expressions of Caspase-3 were determined by Western blot. Cell apoptosis was analyzed by FCM.

RESULTS

The proliferation rate of cells in the P. vulgaris group (67.32 +/- 1.96)% was significantly lower than that in the other groups (P < 0.05). The phosphorylation of JNK (0.48 +/- 0.03) and the phosphorylation of c-Jun (0.46 +/- 0.04) significantly increased in the P. vulgaris group (P < 0.05) and SP600125 inhibited the phosphorylation of c-Jun (0.43 +/- 0.01). The expression of Caspase-3 in the P. vulgaris group (1.35 +/- 0.07) was higher than that in the other groups. And such an effect could be blocked by SP600125 (0.79 +/- 0.06) (P < 0.05). The rate of cellular apoptosis was the highest in the P. vulgaris group (25.32 +/- 5.27)% (P < 0.05).

CONCLUSION

P.vulgaris shows a strong inhibitory effect upon the growth of lymphoma cell line Raji probably through the activation of JNK pathway and caspase channel and then apoptosis.

Authors+Show Affiliations

Department of Case Management, First Affiliated Hospital of Zhengzhou University, Henan Provincial Key Laboratory of Clinical Medicine, Zhengzhou 450052, China.No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

20450729

Citation

Liu, Xin-kui, et al. "[Involvement of JNK and Caspase-3 in Human Lymphoma Cell Apoptosis Induced By Prunella Vulgaris]." Zhonghua Yi Xue Za Zhi, vol. 90, no. 10, 2010, pp. 690-3.
Liu XK, Wang L, Zhang MZ. [Involvement of JNK and caspase-3 in human lymphoma cell apoptosis induced by Prunella vulgaris]. Zhonghua Yi Xue Za Zhi. 2010;90(10):690-3.
Liu, X. K., Wang, L., & Zhang, M. Z. (2010). [Involvement of JNK and caspase-3 in human lymphoma cell apoptosis induced by Prunella vulgaris]. Zhonghua Yi Xue Za Zhi, 90(10), 690-3.
Liu XK, Wang L, Zhang MZ. [Involvement of JNK and Caspase-3 in Human Lymphoma Cell Apoptosis Induced By Prunella Vulgaris]. Zhonghua Yi Xue Za Zhi. 2010 Mar 16;90(10):690-3. PubMed PMID: 20450729.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Involvement of JNK and caspase-3 in human lymphoma cell apoptosis induced by Prunella vulgaris]. AU - Liu,Xin-kui, AU - Wang,Lin, AU - Zhang,Ming-zhi, PY - 2010/5/11/entrez PY - 2010/5/11/pubmed PY - 2010/9/10/medline SP - 690 EP - 3 JF - Zhonghua yi xue za zhi JO - Zhonghua Yi Xue Za Zhi VL - 90 IS - 10 N2 - OBJECTIVE: To characterize the effect of Prunella vulgaris upon lymphoma cells and its possible mechanisms. METHODS: Raji cells were subjected to different therapeutic schemes: Prunella vulgaris (50 g/L) group, P. vulgaris (50 g/L)+SP600125 (20 micromol/L, JNK inhibitor) (SP600125 group); Raji cells in physiological saline served as the control. MTT assay was used to measure the cellular proliferation. Phosphorylation of JNK, c-Jun and the expressions of Caspase-3 were determined by Western blot. Cell apoptosis was analyzed by FCM. RESULTS: The proliferation rate of cells in the P. vulgaris group (67.32 +/- 1.96)% was significantly lower than that in the other groups (P < 0.05). The phosphorylation of JNK (0.48 +/- 0.03) and the phosphorylation of c-Jun (0.46 +/- 0.04) significantly increased in the P. vulgaris group (P < 0.05) and SP600125 inhibited the phosphorylation of c-Jun (0.43 +/- 0.01). The expression of Caspase-3 in the P. vulgaris group (1.35 +/- 0.07) was higher than that in the other groups. And such an effect could be blocked by SP600125 (0.79 +/- 0.06) (P < 0.05). The rate of cellular apoptosis was the highest in the P. vulgaris group (25.32 +/- 5.27)% (P < 0.05). CONCLUSION: P.vulgaris shows a strong inhibitory effect upon the growth of lymphoma cell line Raji probably through the activation of JNK pathway and caspase channel and then apoptosis. SN - 0376-2491 UR - https://www.unboundmedicine.com/medline/citation/20450729/[Involvement_of_JNK_and_caspase_3_in_human_lymphoma_cell_apoptosis_induced_by_Prunella_vulgaris]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=0376-2491&amp;year=2010&amp;vol=90&amp;issue=10&amp;fpage=690 DB - PRIME DP - Unbound Medicine ER -