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Genetic diversity of Greek Aegilops species using different types of nuclear genome markers.
Mol Phylogenet Evol. 2010 Sep; 56(3):951-61.MP

Abstract

Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeat (ISSR) analyses were used to evaluate genetic variability and relationships of Greek Aegilops species. Thirty-eight accessions of seven Greek Aegilops species [Ae. triuncialis (genome UC), Ae. neglecta (UM), Ae. biuncialis (UM), Ae. caudata (C), Ae. comosa (M), Ae. geniculata (MU) and Ae. umbellulata (U)] as well as Triticum accessions were studied. Nineteen RAPD and ten ISSR primers yielded 344 and 170 polymorphic bands, respectively, that were used for the construction of dendrograms. Regardless of the similarity coefficient and marker type used, UPGMA placed 38 Aegilops accessions into one branch while the other branch consisted of wheat species. Within the Aegilops cluster, subgroups were identified that included species that shared the same genome or belonged to the same botanical section. Within the Triticum cluster, two robust subgroups were formed, one including diploid wheat and another including polyploid wheat. In conclusion, results showed that there is genetic diversity in the Greek Aegilops species studied, and clustering based on genetic similarities was in agreement with botanical classifications.

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Authors+Show Affiliations

Thomas KG
Department of Plant Breeding and Biometry, Agricultural University of Athens, Iera Odos 75, Athens 11855, Greece.
Bebeli PJ
No affiliation info available

MeSH

Cell NucleusDNA, PlantEvolution, MolecularGenetic VariationGenome, PlantGeographyGreeceMinisatellite RepeatsPhylogenyPoaceaePolyploidyPrincipal Component AnalysisRandom Amplified Polymorphic DNA TechniqueSequence Analysis, DNATriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20450978

Citation

Thomas, Konstantinos G., and Penelope J. Bebeli. "Genetic Diversity of Greek Aegilops Species Using Different Types of Nuclear Genome Markers." Molecular Phylogenetics and Evolution, vol. 56, no. 3, 2010, pp. 951-61.
Thomas KG, Bebeli PJ. Genetic diversity of Greek Aegilops species using different types of nuclear genome markers. Mol Phylogenet Evol. 2010;56(3):951-61.
Thomas, K. G., & Bebeli, P. J. (2010). Genetic diversity of Greek Aegilops species using different types of nuclear genome markers. Molecular Phylogenetics and Evolution, 56(3), 951-61. https://doi.org/10.1016/j.ympev.2010.04.041
Thomas KG, Bebeli PJ. Genetic Diversity of Greek Aegilops Species Using Different Types of Nuclear Genome Markers. Mol Phylogenet Evol. 2010;56(3):951-61. PubMed PMID: 20450978.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic diversity of Greek Aegilops species using different types of nuclear genome markers. AU - Thomas,Konstantinos G, AU - Bebeli,Penelope J, Y1 - 2010/05/05/ PY - 2009/12/07/received PY - 2010/04/26/revised PY - 2010/04/29/accepted PY - 2010/5/11/entrez PY - 2010/5/11/pubmed PY - 2010/8/24/medline SP - 951 EP - 61 JF - Molecular phylogenetics and evolution JO - Mol Phylogenet Evol VL - 56 IS - 3 N2 - Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeat (ISSR) analyses were used to evaluate genetic variability and relationships of Greek Aegilops species. Thirty-eight accessions of seven Greek Aegilops species [Ae. triuncialis (genome UC), Ae. neglecta (UM), Ae. biuncialis (UM), Ae. caudata (C), Ae. comosa (M), Ae. geniculata (MU) and Ae. umbellulata (U)] as well as Triticum accessions were studied. Nineteen RAPD and ten ISSR primers yielded 344 and 170 polymorphic bands, respectively, that were used for the construction of dendrograms. Regardless of the similarity coefficient and marker type used, UPGMA placed 38 Aegilops accessions into one branch while the other branch consisted of wheat species. Within the Aegilops cluster, subgroups were identified that included species that shared the same genome or belonged to the same botanical section. Within the Triticum cluster, two robust subgroups were formed, one including diploid wheat and another including polyploid wheat. In conclusion, results showed that there is genetic diversity in the Greek Aegilops species studied, and clustering based on genetic similarities was in agreement with botanical classifications. SN - 1095-9513 UR - https://www.unboundmedicine.com/medline/citation/20450978/Genetic_diversity_of_Greek_Aegilops_species_using_different_types_of_nuclear_genome_markers_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1055-7903(10)00207-1 DB - PRIME DP - Unbound Medicine ER -