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Anti-inflammatory effects of sulfuretin from Rhus verniciflua Stokes via the induction of heme oxygenase-1 expression in murine macrophages.
Int Immunopharmacol. 2010 Aug; 10(8):850-8.II

Abstract

Rhus verniciflua Stokes (Anacardiaceae) has traditionally been used as an ingredient in East Asian medicines used to treat oxidative damage and cancer. Sulfuretin is one of the major flavonoid components isolated from R. verniciflua. In the present study, we isolated sulfuretin from R. verniciflua and demonstrated that sulfuretin inhibited inducible nitric oxide synthase (iNOS) protein and mRNA expression, reduced iNOS-derived NO, suppressed COX-2 protein and mRNA expression, and reduced COX-derived PGE(2) production in lipopolysaccharide (LPS)-stimulated RAW264.7 and murine peritoneal macrophages. Similarly, sulfuretin reduced tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) production. In addition, sulfuretin suppressed the phosphorylation and degradation of I kappaB-alpha as well as the nuclear translocation of p65 by the stimulation of LPS in RAW264.7 macrophages. Furthermore sulfuretin induced heme oxygenase (HO)-1 expression through nuclear translocation of nuclear factor E2-related factor 2 (Nrf)2 and increased heme oxygenase (HO) activity in RAW264.7 macrophages. The effects of sulfuretin on LPS-induced NO, PGE(2), TNF-alpha, and IL-1 beta production were partially reversed by the HO-1 inhibitor, tin protoporphyrin (SnPP). Therefore, it is suggested that sulfuretin-induced HO-1 expression plays a role of the resulting anti-inflammatory effects in macrophages. This indicated that the anti-inflammatory effects of sulfuretin in macrophages might be exerted through a novel mechanism that involves HO-1 expression.

Authors+Show Affiliations

College of Pharmacy, Wonkwang University, Iksan, Jeonbuk 570-749, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20450988

Citation

Lee, Dong-Sung, et al. "Anti-inflammatory Effects of Sulfuretin From Rhus Verniciflua Stokes Via the Induction of Heme Oxygenase-1 Expression in Murine Macrophages." International Immunopharmacology, vol. 10, no. 8, 2010, pp. 850-8.
Lee DS, Jeong GS, Li B, et al. Anti-inflammatory effects of sulfuretin from Rhus verniciflua Stokes via the induction of heme oxygenase-1 expression in murine macrophages. Int Immunopharmacol. 2010;10(8):850-8.
Lee, D. S., Jeong, G. S., Li, B., Park, H., & Kim, Y. C. (2010). Anti-inflammatory effects of sulfuretin from Rhus verniciflua Stokes via the induction of heme oxygenase-1 expression in murine macrophages. International Immunopharmacology, 10(8), 850-8. https://doi.org/10.1016/j.intimp.2010.04.019
Lee DS, et al. Anti-inflammatory Effects of Sulfuretin From Rhus Verniciflua Stokes Via the Induction of Heme Oxygenase-1 Expression in Murine Macrophages. Int Immunopharmacol. 2010;10(8):850-8. PubMed PMID: 20450988.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-inflammatory effects of sulfuretin from Rhus verniciflua Stokes via the induction of heme oxygenase-1 expression in murine macrophages. AU - Lee,Dong-Sung, AU - Jeong,Gil-Saeng, AU - Li,Bin, AU - Park,Hyun, AU - Kim,Youn-Chul, Y1 - 2010/05/05/ PY - 2009/11/11/received PY - 2010/04/01/revised PY - 2010/04/26/accepted PY - 2010/5/11/entrez PY - 2010/5/11/pubmed PY - 2011/3/4/medline SP - 850 EP - 8 JF - International immunopharmacology JO - Int Immunopharmacol VL - 10 IS - 8 N2 - Rhus verniciflua Stokes (Anacardiaceae) has traditionally been used as an ingredient in East Asian medicines used to treat oxidative damage and cancer. Sulfuretin is one of the major flavonoid components isolated from R. verniciflua. In the present study, we isolated sulfuretin from R. verniciflua and demonstrated that sulfuretin inhibited inducible nitric oxide synthase (iNOS) protein and mRNA expression, reduced iNOS-derived NO, suppressed COX-2 protein and mRNA expression, and reduced COX-derived PGE(2) production in lipopolysaccharide (LPS)-stimulated RAW264.7 and murine peritoneal macrophages. Similarly, sulfuretin reduced tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) production. In addition, sulfuretin suppressed the phosphorylation and degradation of I kappaB-alpha as well as the nuclear translocation of p65 by the stimulation of LPS in RAW264.7 macrophages. Furthermore sulfuretin induced heme oxygenase (HO)-1 expression through nuclear translocation of nuclear factor E2-related factor 2 (Nrf)2 and increased heme oxygenase (HO) activity in RAW264.7 macrophages. The effects of sulfuretin on LPS-induced NO, PGE(2), TNF-alpha, and IL-1 beta production were partially reversed by the HO-1 inhibitor, tin protoporphyrin (SnPP). Therefore, it is suggested that sulfuretin-induced HO-1 expression plays a role of the resulting anti-inflammatory effects in macrophages. This indicated that the anti-inflammatory effects of sulfuretin in macrophages might be exerted through a novel mechanism that involves HO-1 expression. SN - 1878-1705 UR - https://www.unboundmedicine.com/medline/citation/20450988/Anti_inflammatory_effects_of_sulfuretin_from_Rhus_verniciflua_Stokes_via_the_induction_of_heme_oxygenase_1_expression_in_murine_macrophages_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(10)00133-5 DB - PRIME DP - Unbound Medicine ER -