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Polyphenolic apple extracts: effects of raw material and production method on antioxidant effectiveness and reduction of DNA damage in Caco-2 cells.
J Agric Food Chem. 2010 Jun 09; 58(11):6636-42.JA

Abstract

A diet rich in fruits and vegetables is commonly perceived to be associated with reduced cancer risk, attributed to its high content of polyphenols. As apples represent a major polyphenol source in Western countries, we studied differentially produced extracts (1-100 microg/mL): two from different apple juices (AEs), one from pomace (APE), and one peel extract (PE) on their potential to reduce DNA oxidation damage and induce antioxidant defense in Caco-2 cells. Additionally, we measured direct antioxidant capacity (TEAC/ORAC) of the extracts. Quercetin-rich PE and APE most effectively diminished DNA damage and ROS level after 24 h incubation (PE > APE), whereas the AEs were only moderately effective. GPx activity was diminished for all extracts, with AEs > APE > PE. Direct antioxidant activity decreased in the order AEs > PE > APE, displaying no significant correlation with cellular markers. In conclusion, apple phenolics at low, nutritionally relevant concentrations may protect intestinal cells from ROS-induced DNA damage, mediated by cellular defense mechanisms rather than by antioxidant activity.

Authors+Show Affiliations

Department of Chemistry, Division of Food Chemistry & Toxicology, University of Kaiserslautern, Erwin Schroedinger Strasse 52, 67663 Kaiserslautern, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20455581

Citation

Bellion, Phillip, et al. "Polyphenolic Apple Extracts: Effects of Raw Material and Production Method On Antioxidant Effectiveness and Reduction of DNA Damage in Caco-2 Cells." Journal of Agricultural and Food Chemistry, vol. 58, no. 11, 2010, pp. 6636-42.
Bellion P, Digles J, Will F, et al. Polyphenolic apple extracts: effects of raw material and production method on antioxidant effectiveness and reduction of DNA damage in Caco-2 cells. J Agric Food Chem. 2010;58(11):6636-42.
Bellion, P., Digles, J., Will, F., Dietrich, H., Baum, M., Eisenbrand, G., & Janzowski, C. (2010). Polyphenolic apple extracts: effects of raw material and production method on antioxidant effectiveness and reduction of DNA damage in Caco-2 cells. Journal of Agricultural and Food Chemistry, 58(11), 6636-42. https://doi.org/10.1021/jf904150x
Bellion P, et al. Polyphenolic Apple Extracts: Effects of Raw Material and Production Method On Antioxidant Effectiveness and Reduction of DNA Damage in Caco-2 Cells. J Agric Food Chem. 2010 Jun 9;58(11):6636-42. PubMed PMID: 20455581.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polyphenolic apple extracts: effects of raw material and production method on antioxidant effectiveness and reduction of DNA damage in Caco-2 cells. AU - Bellion,Phillip, AU - Digles,Jasmin, AU - Will,Frank, AU - Dietrich,Helmut, AU - Baum,Matthias, AU - Eisenbrand,Gerhard, AU - Janzowski,Christine, PY - 2010/5/12/entrez PY - 2010/5/12/pubmed PY - 2010/8/28/medline SP - 6636 EP - 42 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 58 IS - 11 N2 - A diet rich in fruits and vegetables is commonly perceived to be associated with reduced cancer risk, attributed to its high content of polyphenols. As apples represent a major polyphenol source in Western countries, we studied differentially produced extracts (1-100 microg/mL): two from different apple juices (AEs), one from pomace (APE), and one peel extract (PE) on their potential to reduce DNA oxidation damage and induce antioxidant defense in Caco-2 cells. Additionally, we measured direct antioxidant capacity (TEAC/ORAC) of the extracts. Quercetin-rich PE and APE most effectively diminished DNA damage and ROS level after 24 h incubation (PE > APE), whereas the AEs were only moderately effective. GPx activity was diminished for all extracts, with AEs > APE > PE. Direct antioxidant activity decreased in the order AEs > PE > APE, displaying no significant correlation with cellular markers. In conclusion, apple phenolics at low, nutritionally relevant concentrations may protect intestinal cells from ROS-induced DNA damage, mediated by cellular defense mechanisms rather than by antioxidant activity. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/20455581/Polyphenolic_apple_extracts:_effects_of_raw_material_and_production_method_on_antioxidant_effectiveness_and_reduction_of_DNA_damage_in_Caco_2_cells_ L2 - https://doi.org/10.1021/jf904150x DB - PRIME DP - Unbound Medicine ER -