Abstract
BACKGROUND/PURPOSE
Dermal water plays an important role in the physical properties of the skin. Recently, researchers have attempted to directly measure the dermal water content in vivo using magnetic resonance imaging, near infrared spectroscopy, and Raman spectroscopy. However, these methods have limitations. Although confocal Raman spectroscopy has been developed to measure the water content in the skin, no reports have suggested that this instrument can measure the dermal water content. This report describes a method for measuring the dermal water content in vivo using confocal Raman spectroscopy.
METHODS
We used a confocal Raman spectrometer and adjusted the laser exposure time and depth increments according to the skin depth. Age-related changes in the dermal water content of the forearm were examined in 30 young and 30 elderly male subjects. Diurnal changes in the dermal water content of the forearm were examined in 12 elderly male subjects.
RESULTS
Adjusting the exposure time and depth increment dramatically improved the signal-to-noise ratios of the Raman spectra. Elderly dermis had significantly higher water content than young dermis. Moreover, the dermal water content displayed a diurnal change.
CONCLUSION
This study demonstrates that the dermal water content can be measured in vivo using confocal Raman spectroscopy.
TY - JOUR
T1 - In vivo measurement of the water content in the dermis by confocal Raman spectroscopy.
AU - Nakagawa,Noriaki,
AU - Matsumoto,Masayuki,
AU - Sakai,Shingo,
PY - 2010/5/12/entrez
PY - 2010/5/12/pubmed
PY - 2010/8/14/medline
SP - 137
EP - 41
JF - Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)
JO - Skin Res Technol
VL - 16
IS - 2
N2 - BACKGROUND/PURPOSE: Dermal water plays an important role in the physical properties of the skin. Recently, researchers have attempted to directly measure the dermal water content in vivo using magnetic resonance imaging, near infrared spectroscopy, and Raman spectroscopy. However, these methods have limitations. Although confocal Raman spectroscopy has been developed to measure the water content in the skin, no reports have suggested that this instrument can measure the dermal water content. This report describes a method for measuring the dermal water content in vivo using confocal Raman spectroscopy. METHODS: We used a confocal Raman spectrometer and adjusted the laser exposure time and depth increments according to the skin depth. Age-related changes in the dermal water content of the forearm were examined in 30 young and 30 elderly male subjects. Diurnal changes in the dermal water content of the forearm were examined in 12 elderly male subjects. RESULTS: Adjusting the exposure time and depth increment dramatically improved the signal-to-noise ratios of the Raman spectra. Elderly dermis had significantly higher water content than young dermis. Moreover, the dermal water content displayed a diurnal change. CONCLUSION: This study demonstrates that the dermal water content can be measured in vivo using confocal Raman spectroscopy.
SN - 1600-0846
UR - https://www.unboundmedicine.com/medline/citation/20456092/In_vivo_measurement_of_the_water_content_in_the_dermis_by_confocal_Raman_spectroscopy_
L2 - https://doi.org/10.1111/j.1600-0846.2009.00410.x
DB - PRIME
DP - Unbound Medicine
ER -
