TY - JOUR T1 - Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43. AU - Lee,Hyun Kyoung, AU - Lee,Byoung Hee, AU - Seok,Seung Hyeok, AU - Baek,Min Won, AU - Lee,Hui Young, AU - Kim,Dong Jae, AU - Na,Yi Rang, AU - Noh,Kyoung Jin, AU - Park,Sung Hoon, AU - Kumar,Dutta Noton, AU - Kariwa,Hiroaki, AU - Nakauchi,Mina, AU - Heo,Suk Jin, AU - Park,Jae Hak, PY - 2010/5/12/entrez PY - 2010/5/12/pubmed PY - 2011/1/22/medline SP - 165 EP - 7 JF - Journal of veterinary science JO - J Vet Sci VL - 11 IS - 2 N2 - Severe acute respiratory syndrome (SARS) is a life-threatening disease for which accurate diagnosis is essential. Although many tools have been developed for the diagnosis of SARS, false-positive reactions in negative sera may occur because of cross-reactivity with other coronaviruses. We have raised polyclonal and monoclonal antibodies (Abs) using a recombinant form of the SARS virus nucleocapsid protein. Cross-reactivity of these anti-SARS Abs against human coronavirus (HCoV) 229E and HCoV OC43 were determined by Western blotting. The Abs produced reacted with recombinant SARS virus nucleocapsid protein, but not with HCoV 229E or HCoV OC43. SN - 1976-555X UR - https://www.unboundmedicine.com/medline/citation/20458159/Production_of_specific_antibodies_against_SARS_coronavirus_nucleocapsid_protein_without_cross_reactivity_with_human_coronaviruses_229E_and_OC43_ L2 - https://www.vetsci.org/DOIx.php?id=10.4142/jvs.2010.11.2.165 DB - PRIME DP - Unbound Medicine ER -