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Glucosamine promotes osteogenic differentiation of dental pulp stem cells through modulating the level of the transforming growth factor-beta type I receptor.
J Cell Physiol. 2010 Oct; 225(1):140-51.JC

Abstract

Dental pulp stem cells (DPSCs) are clonogenic, self-renewing, and multi-potential DPSCs capable of differentiating into osteoblasts. In this study, primary cell cultures were obtained from human dental pulp tissue of developing third molars, and flow cytometry was used to sort the subpopulation of DPSCs with STRO-1 and CD146 double-positive expression (denoted "DPSCs"). It was noted that DPSCs exhibited superior clonogenic potential and osteogenic differentiation capability than the dental pulp cell subpopulation with STRO-1 and CD146 double-negative expression (denoted DPCs). Furthermore, a low concentration (0.005 mg/ml) of exogenous glucosamine (GlcN) was effective in promoting the early osteogenic differentiation of DPSCs through the transforming growth factor-beta receptor (TGF-betar) type I and Smads signal pathways, which upregulated the Runt-related transcription factor 2/core-binding factor alpha1 (Runx2/Cbfa1) and alkaline phosphatase at both the mRNA and protein levels. In the presence of osteogenic supplements, GlcN-treated DPSCs produced more mineralized-matrix deposition than did the untreated groups. Taken together, this study demonstrates the capacity of GlcN to promote the osteogenic differentiation of human DPSCs, and the underlying mechanism involves a TGF-betar-dependent Smad signal pathway.

Authors+Show Affiliations

Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20458730

Citation

Huang, Chien-Hsun, et al. "Glucosamine Promotes Osteogenic Differentiation of Dental Pulp Stem Cells Through Modulating the Level of the Transforming Growth Factor-beta Type I Receptor." Journal of Cellular Physiology, vol. 225, no. 1, 2010, pp. 140-51.
Huang CH, Tseng WY, Yao CC, et al. Glucosamine promotes osteogenic differentiation of dental pulp stem cells through modulating the level of the transforming growth factor-beta type I receptor. J Cell Physiol. 2010;225(1):140-51.
Huang, C. H., Tseng, W. Y., Yao, C. C., Jeng, J. H., Young, T. H., & Chen, Y. J. (2010). Glucosamine promotes osteogenic differentiation of dental pulp stem cells through modulating the level of the transforming growth factor-beta type I receptor. Journal of Cellular Physiology, 225(1), 140-51. https://doi.org/10.1002/jcp.22206
Huang CH, et al. Glucosamine Promotes Osteogenic Differentiation of Dental Pulp Stem Cells Through Modulating the Level of the Transforming Growth Factor-beta Type I Receptor. J Cell Physiol. 2010;225(1):140-51. PubMed PMID: 20458730.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glucosamine promotes osteogenic differentiation of dental pulp stem cells through modulating the level of the transforming growth factor-beta type I receptor. AU - Huang,Chien-Hsun, AU - Tseng,Wan-Yu, AU - Yao,Chung-Chen, AU - Jeng,Jiiang-Huei, AU - Young,Tai-Horng, AU - Chen,Yi-Jane, PY - 2010/5/12/entrez PY - 2010/5/12/pubmed PY - 2010/9/16/medline SP - 140 EP - 51 JF - Journal of cellular physiology JO - J. Cell. Physiol. VL - 225 IS - 1 N2 - Dental pulp stem cells (DPSCs) are clonogenic, self-renewing, and multi-potential DPSCs capable of differentiating into osteoblasts. In this study, primary cell cultures were obtained from human dental pulp tissue of developing third molars, and flow cytometry was used to sort the subpopulation of DPSCs with STRO-1 and CD146 double-positive expression (denoted "DPSCs"). It was noted that DPSCs exhibited superior clonogenic potential and osteogenic differentiation capability than the dental pulp cell subpopulation with STRO-1 and CD146 double-negative expression (denoted DPCs). Furthermore, a low concentration (0.005 mg/ml) of exogenous glucosamine (GlcN) was effective in promoting the early osteogenic differentiation of DPSCs through the transforming growth factor-beta receptor (TGF-betar) type I and Smads signal pathways, which upregulated the Runt-related transcription factor 2/core-binding factor alpha1 (Runx2/Cbfa1) and alkaline phosphatase at both the mRNA and protein levels. In the presence of osteogenic supplements, GlcN-treated DPSCs produced more mineralized-matrix deposition than did the untreated groups. Taken together, this study demonstrates the capacity of GlcN to promote the osteogenic differentiation of human DPSCs, and the underlying mechanism involves a TGF-betar-dependent Smad signal pathway. SN - 1097-4652 UR - https://www.unboundmedicine.com/medline/citation/20458730/Glucosamine_promotes_osteogenic_differentiation_of_dental_pulp_stem_cells_through_modulating_the_level_of_the_transforming_growth_factor_beta_type_I_receptor_ L2 - https://doi.org/10.1002/jcp.22206 DB - PRIME DP - Unbound Medicine ER -