Tags

Type your tag names separated by a space and hit enter

Allyl sulfides inhibit cell growth of skin cancer cells through induction of DNA damage mediated G2/M arrest and apoptosis.
J Agric Food Chem 2010; 58(11):7096-103JA

Abstract

Diallyl sulfide (DAS), diallyl disulfide (DADS), and diallyl trisulfide (DATS), extracted from crushed garlic by steam-distillation, have been reported to provide the anticancer activity in several cancer types. However, their mechanisms of effects on skin cancer cells remain unclear. Therefore, we used human melanoma A375 cells and basal cell carcinoma cells as the models to elucidate the effects of these three allyl sulfides. Basal cell carcinoma (BCC) is known to be the most prevalent type of skin cancer, and melanoma is the most lethal form. We found that DATS revealed better growth inhibition of A375 and BCC cells than DADS and DAS did. We further demonstrated that DATS increased intracellular reactive oxygen species (ROS) generation, induced cytosolic Ca(2+) mobilization, and decreased mitochondrial membrane potential (DeltaPsim). Western blot results showed the concordance for the expression of molecules involved in G(2)/M arrest and apoptosis observed by cell cycle and cell viability analysis. Moreover, we detected the activation of p53 pathway in response to the oxidative DNA damage. DATS also displayed selective target of growth inhibition between skin cancer cells and normal keratinocyte HaCaT cells. Taken together, these results suggest that DATS is a potential anticancer compound for skin cancer.

Authors+Show Affiliations

Graduate Institute of Food Science and Technology, National Taiwan University, No 1, Sec 4, Roosevelt Road, Taipei 106, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20459099

Citation

Wang, Hsiao Chi, et al. "Allyl Sulfides Inhibit Cell Growth of Skin Cancer Cells Through Induction of DNA Damage Mediated G2/M Arrest and Apoptosis." Journal of Agricultural and Food Chemistry, vol. 58, no. 11, 2010, pp. 7096-103.
Wang HC, Yang JH, Hsieh SC, et al. Allyl sulfides inhibit cell growth of skin cancer cells through induction of DNA damage mediated G2/M arrest and apoptosis. J Agric Food Chem. 2010;58(11):7096-103.
Wang, H. C., Yang, J. H., Hsieh, S. C., & Sheen, L. Y. (2010). Allyl sulfides inhibit cell growth of skin cancer cells through induction of DNA damage mediated G2/M arrest and apoptosis. Journal of Agricultural and Food Chemistry, 58(11), pp. 7096-103. doi:10.1021/jf100613x.
Wang HC, et al. Allyl Sulfides Inhibit Cell Growth of Skin Cancer Cells Through Induction of DNA Damage Mediated G2/M Arrest and Apoptosis. J Agric Food Chem. 2010 Jun 9;58(11):7096-103. PubMed PMID: 20459099.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Allyl sulfides inhibit cell growth of skin cancer cells through induction of DNA damage mediated G2/M arrest and apoptosis. AU - Wang,Hsiao Chi, AU - Yang,Jen-Hung, AU - Hsieh,Shu-Chen, AU - Sheen,Lee-Yan, PY - 2010/5/13/entrez PY - 2010/5/13/pubmed PY - 2010/8/28/medline SP - 7096 EP - 103 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 58 IS - 11 N2 - Diallyl sulfide (DAS), diallyl disulfide (DADS), and diallyl trisulfide (DATS), extracted from crushed garlic by steam-distillation, have been reported to provide the anticancer activity in several cancer types. However, their mechanisms of effects on skin cancer cells remain unclear. Therefore, we used human melanoma A375 cells and basal cell carcinoma cells as the models to elucidate the effects of these three allyl sulfides. Basal cell carcinoma (BCC) is known to be the most prevalent type of skin cancer, and melanoma is the most lethal form. We found that DATS revealed better growth inhibition of A375 and BCC cells than DADS and DAS did. We further demonstrated that DATS increased intracellular reactive oxygen species (ROS) generation, induced cytosolic Ca(2+) mobilization, and decreased mitochondrial membrane potential (DeltaPsim). Western blot results showed the concordance for the expression of molecules involved in G(2)/M arrest and apoptosis observed by cell cycle and cell viability analysis. Moreover, we detected the activation of p53 pathway in response to the oxidative DNA damage. DATS also displayed selective target of growth inhibition between skin cancer cells and normal keratinocyte HaCaT cells. Taken together, these results suggest that DATS is a potential anticancer compound for skin cancer. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/20459099/Allyl_sulfides_inhibit_cell_growth_of_skin_cancer_cells_through_induction_of_DNA_damage_mediated_G2/M_arrest_and_apoptosis_ L2 - https://dx.doi.org/10.1021/jf100613x DB - PRIME DP - Unbound Medicine ER -