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Tea polyphenols inhibit IL-6 production in tumor necrosis factor superfamily 14-stimulated human gingival fibroblasts.

Abstract

IL-6 is well recognized to be a potent bone resorptive agent and thus in the development of periodontal disease. Epigallocatechin gallate (EGCG) and epicatechin gallate (ECG), the major catechins in green tea, and theaflavin-3,3'-digallate (TFDG), polyphenol in black tea, have multiple beneficial effects, but the effects of catechins and theaflavins on IL-6 production in human gingival fibroblasts (HGFs) are not known. In this study, we investigated the mechanisms by which EGCG, ECG, and TFDG inhibit tumor necrosis factor superfamily 14 (TNFSF14)-induced IL-6 production in HGFs. We detected TNFSF14 mRNA expression in human diseased periodontal tissues. TNFSF14 increased IL-6 production in HGFs in a concentration-dependent manner. EGCG, ECG, and TFDG prevented TNFSF14-mediated IL-6 production in HGFs. EGCG, ECG, and TFDG prevented TNFSF14-induced extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and nuclear factor-kappaB activation in HGFs. Inhibitors of ERK, JNK, and nuclear factor-kappaB decreased TNFSF14-induced IL-6 production. In addition, EGCG, ECG, and TFDG attenuated TNFSF14 receptor expression on HGFs. These data provide a novel mechanism through which the green tea and black tea polyphenols could be used to provide direct benefits in periodontal disease.

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  • Authors+Show Affiliations

    ,

    Department of Conservative Dentistry, The University of Tokushima Graduate School, Tokushima, Japan. hosokawa@dent.tokushima-u.ac.jp

    , , , ,

    Source

    Molecular nutrition & food research 54 Suppl 2: 2010 Jul pg S151-8

    MeSH

    Aged
    Anti-Inflammatory Agents, Non-Steroidal
    Antioxidants
    Biflavonoids
    Catechin
    Cells, Cultured
    Chronic Periodontitis
    Enzyme Inhibitors
    Female
    Fibroblasts
    Flavonoids
    Gallic Acid
    Gene Expression Regulation
    Gingiva
    Humans
    Interleukin-6
    Male
    Middle Aged
    Phenols
    Polyphenols
    RNA, Messenger
    Signal Transduction
    Tea
    Tumor Necrosis Factor Ligand Superfamily Member 14

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    20461739

    Citation

    Hosokawa, Yoshitaka, et al. "Tea Polyphenols Inhibit IL-6 Production in Tumor Necrosis Factor Superfamily 14-stimulated Human Gingival Fibroblasts." Molecular Nutrition & Food Research, vol. 54 Suppl 2, 2010, pp. S151-8.
    Hosokawa Y, Hosokawa I, Ozaki K, et al. Tea polyphenols inhibit IL-6 production in tumor necrosis factor superfamily 14-stimulated human gingival fibroblasts. Mol Nutr Food Res. 2010;54 Suppl 2:S151-8.
    Hosokawa, Y., Hosokawa, I., Ozaki, K., Nakanishi, T., Nakae, H., & Matsuo, T. (2010). Tea polyphenols inhibit IL-6 production in tumor necrosis factor superfamily 14-stimulated human gingival fibroblasts. Molecular Nutrition & Food Research, 54 Suppl 2, pp. S151-8. doi:10.1002/mnfr.200900549.
    Hosokawa Y, et al. Tea Polyphenols Inhibit IL-6 Production in Tumor Necrosis Factor Superfamily 14-stimulated Human Gingival Fibroblasts. Mol Nutr Food Res. 2010;54 Suppl 2:S151-8. PubMed PMID: 20461739.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Tea polyphenols inhibit IL-6 production in tumor necrosis factor superfamily 14-stimulated human gingival fibroblasts. AU - Hosokawa,Yoshitaka, AU - Hosokawa,Ikuko, AU - Ozaki,Kazumi, AU - Nakanishi,Tadashi, AU - Nakae,Hideaki, AU - Matsuo,Takashi, PY - 2010/5/13/entrez PY - 2010/5/13/pubmed PY - 2010/12/14/medline SP - S151 EP - 8 JF - Molecular nutrition & food research JO - Mol Nutr Food Res VL - 54 Suppl 2 N2 - IL-6 is well recognized to be a potent bone resorptive agent and thus in the development of periodontal disease. Epigallocatechin gallate (EGCG) and epicatechin gallate (ECG), the major catechins in green tea, and theaflavin-3,3'-digallate (TFDG), polyphenol in black tea, have multiple beneficial effects, but the effects of catechins and theaflavins on IL-6 production in human gingival fibroblasts (HGFs) are not known. In this study, we investigated the mechanisms by which EGCG, ECG, and TFDG inhibit tumor necrosis factor superfamily 14 (TNFSF14)-induced IL-6 production in HGFs. We detected TNFSF14 mRNA expression in human diseased periodontal tissues. TNFSF14 increased IL-6 production in HGFs in a concentration-dependent manner. EGCG, ECG, and TFDG prevented TNFSF14-mediated IL-6 production in HGFs. EGCG, ECG, and TFDG prevented TNFSF14-induced extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and nuclear factor-kappaB activation in HGFs. Inhibitors of ERK, JNK, and nuclear factor-kappaB decreased TNFSF14-induced IL-6 production. In addition, EGCG, ECG, and TFDG attenuated TNFSF14 receptor expression on HGFs. These data provide a novel mechanism through which the green tea and black tea polyphenols could be used to provide direct benefits in periodontal disease. SN - 1613-4133 UR - https://www.unboundmedicine.com/medline/citation/20461739/Tea_polyphenols_inhibit_IL_6_production_in_tumor_necrosis_factor_superfamily_14_stimulated_human_gingival_fibroblasts_ L2 - https://doi.org/10.1002/mnfr.200900549 DB - PRIME DP - Unbound Medicine ER -