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Rapid isolation with Sep-Pak C18 cartridges and wide-bore capillary gas chromatography of benzophenones, the acid-hydrolysis products of benzodiazepines.
J Anal Toxicol. 1991 Jan-Feb; 15(1):21-4.JA

Abstract

A simple extraction procedure with a Sep-Pak C18 cartridge for benzophenones and their detection after separation by wide-bore capillary gas chromatography (GC) are presented. The benzodiazepine-containing samples, after heating in acid and centrifugation, were passed through the cartridges and eluted with chloroform. Except for bromazepam, the recoveries of benzodiazepines detected as benzophenones were excellent. Separation of each benzophenone peak from impurities was achieved with an intermediately polar HP-17 capillary column. Electron capture detection gave sensitivity more than 10 times higher than that of flame ionization detection.

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Authors+Show Affiliations

Seno H
Department of Legal Medicine, Hamamatsu University School of Medicine, Japan.
Suzuki O
No affiliation info available
Kumazawa T
No affiliation info available
Hattori H
No affiliation info available

MeSH

AcidsBenzodiazepinesBenzophenonesChromatography, GasFlame IonizationHumansHydrolysisMolecular StructureTime Factors

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2046337

Citation

Seno, H, et al. "Rapid Isolation With Sep-Pak C18 Cartridges and Wide-bore Capillary Gas Chromatography of Benzophenones, the Acid-hydrolysis Products of Benzodiazepines." Journal of Analytical Toxicology, vol. 15, no. 1, 1991, pp. 21-4.
Seno H, Suzuki O, Kumazawa T, et al. Rapid isolation with Sep-Pak C18 cartridges and wide-bore capillary gas chromatography of benzophenones, the acid-hydrolysis products of benzodiazepines. J Anal Toxicol. 1991;15(1):21-4.
Seno, H., Suzuki, O., Kumazawa, T., & Hattori, H. (1991). Rapid isolation with Sep-Pak C18 cartridges and wide-bore capillary gas chromatography of benzophenones, the acid-hydrolysis products of benzodiazepines. Journal of Analytical Toxicology, 15(1), 21-4.
Seno H, et al. Rapid Isolation With Sep-Pak C18 Cartridges and Wide-bore Capillary Gas Chromatography of Benzophenones, the Acid-hydrolysis Products of Benzodiazepines. J Anal Toxicol. 1991 Jan-Feb;15(1):21-4. PubMed PMID: 2046337.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid isolation with Sep-Pak C18 cartridges and wide-bore capillary gas chromatography of benzophenones, the acid-hydrolysis products of benzodiazepines. AU - Seno,H, AU - Suzuki,O, AU - Kumazawa,T, AU - Hattori,H, PY - 1991/1/1/pubmed PY - 1991/1/1/medline PY - 1991/1/1/entrez SP - 21 EP - 4 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 15 IS - 1 N2 - A simple extraction procedure with a Sep-Pak C18 cartridge for benzophenones and their detection after separation by wide-bore capillary gas chromatography (GC) are presented. The benzodiazepine-containing samples, after heating in acid and centrifugation, were passed through the cartridges and eluted with chloroform. Except for bromazepam, the recoveries of benzodiazepines detected as benzophenones were excellent. Separation of each benzophenone peak from impurities was achieved with an intermediately polar HP-17 capillary column. Electron capture detection gave sensitivity more than 10 times higher than that of flame ionization detection. SN - 0146-4760 UR - https://www.unboundmedicine.com/medline/citation/2046337/Rapid_isolation_with_Sep_Pak_C18_cartridges_and_wide_bore_capillary_gas_chromatography_of_benzophenones_the_acid_hydrolysis_products_of_benzodiazepines_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/15.1.21 DB - PRIME DP - Unbound Medicine ER -