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Molecular detection of rhinoviruses.
Expert Rev Mol Diagn. 2010 May; 10(4):395-8.ER

Abstract

Rhinoviruses are a frequent cause of the common cold and more serious illnesses, such as otitis media, sinusitis and asthma exacerbations and, more infrequently, have been implicated in lower airway infections, including pneumonia and exacerbations of chronic obstructive pulmonary disease and cystic fibrosis. Diagnosis of rhinovirus infections is best achieved using sensitive and specific reverse-transcription (RT)-PCR assays. The article by Do et al. describes the development and validation of a new one-step, TaqMan RT-PCR assay targeting the rhinovirus noncoding region for the detection of rhinovirus RNA in respiratory specimens from children with upper respiratory symptoms. The assay was rapid and specific and sensitive compared with cell culture. The recent use of RT-PCR assays for the detection of rhinoviruses in clinical specimens has provided more accurate information about the disease burden and epidemiology of these ubiquitous viruses.

Authors+Show Affiliations

Department of Laboratory Medicine, University of Washington, 1616 Eastlake Ave E., Suite 320, Seattle, WA 98102, USA. kuypers@u.washington.edu

Pub Type(s)

Comment
Journal Article

Language

eng

PubMed ID

20465495

Citation

Kuypers, Jane. "Molecular Detection of Rhinoviruses." Expert Review of Molecular Diagnostics, vol. 10, no. 4, 2010, pp. 395-8.
Kuypers J. Molecular detection of rhinoviruses. Expert Rev Mol Diagn. 2010;10(4):395-8.
Kuypers, J. (2010). Molecular detection of rhinoviruses. Expert Review of Molecular Diagnostics, 10(4), 395-8. https://doi.org/10.1586/erm.10.38
Kuypers J. Molecular Detection of Rhinoviruses. Expert Rev Mol Diagn. 2010;10(4):395-8. PubMed PMID: 20465495.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular detection of rhinoviruses. A1 - Kuypers,Jane, PY - 2010/5/15/entrez PY - 2010/5/15/pubmed PY - 2010/5/15/medline SP - 395 EP - 8 JF - Expert review of molecular diagnostics JO - Expert Rev Mol Diagn VL - 10 IS - 4 N2 - Rhinoviruses are a frequent cause of the common cold and more serious illnesses, such as otitis media, sinusitis and asthma exacerbations and, more infrequently, have been implicated in lower airway infections, including pneumonia and exacerbations of chronic obstructive pulmonary disease and cystic fibrosis. Diagnosis of rhinovirus infections is best achieved using sensitive and specific reverse-transcription (RT)-PCR assays. The article by Do et al. describes the development and validation of a new one-step, TaqMan RT-PCR assay targeting the rhinovirus noncoding region for the detection of rhinovirus RNA in respiratory specimens from children with upper respiratory symptoms. The assay was rapid and specific and sensitive compared with cell culture. The recent use of RT-PCR assays for the detection of rhinoviruses in clinical specimens has provided more accurate information about the disease burden and epidemiology of these ubiquitous viruses. SN - 1744-8352 UR - https://www.unboundmedicine.com/medline/citation/20465495/Molecular_detection_of_rhinoviruses_ L2 - https://www.tandfonline.com/doi/full/10.1586/erm.10.38 DB - PRIME DP - Unbound Medicine ER -
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