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[Effect of amygdalin on serum proteinic biomarker in pulmonary fibrosis of bleomycin-induced rat].

Abstract

OBJECTIVE

To evaluate effect of amygdalin on expression of four biomarkers in the animal model of pulmonary fibrosis induced by bleomycin.

METHODS

Rats were given one dose (5 mg/kg) of bleomycin in bleomycin-treated groups, amygdalin-treated groups and saline in controls by intratracheal instillation exposed surgically. The amygdalin-treated groups rats were treated with intraperitoneal injection of amygdalin (15 mg x kg(-1) x day(-1)). The rats were sacrificed 7, 14 and 28 days after bleomycin administration. Polarized light microscopy and Image-Pro Plus detected I and III collagen expressed in Paraffin-embedded lung sections stained with Sirius red. Surface-enhanced laser desorption-ionization time-of-flight mass spectrometry (SELDI-TOF MS) with weak cationic proteinchip (CM10) detected differentially expressed proteins in the pooled serum samples of all groups.

RESULTS

Consistent fibrotic responses were found in all bleomycin and amygdalin-tread groups. On the 7th, 14th and 28th day after bleomycin or saline instillation, four differentially expressed proteins were detected in the pooled serum of all groups rats, consisting of 4 proteins with mass/charge ratio of 3530.7, 7043.5, 8332.6 and 9068.0, respectively. Compared with control groups, protein peaks intensity ratio with mass/charge ratio of 3530.7 on 7, 28 d and 7043.5, 8332.6 and 9068.0 on 7, 14 and 28 d was > 2 in bleomycin-treated groups. Compared with amygdalin-treated groups, protein peaks intensity with mass/charge ratio of 3530.7 at 7, 14, 28 d had no change almost, but protein peaks intensity ratio with mass/charge ratio of 7043.5 at 7 d, 8332.6 on 28 d and 9068.0 on 14 d was > 2 in bleomycin-tread groups. All the four protein peaks intensity had no change almost at other point.

CONCLUSION

Amygdalin may reduce the bleomycin-induced increase of differentially expressed protein peak intensities in rat serum.

Authors+Show Affiliations

General Hospital of PLA, Beijing 100853, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

chi

PubMed ID

20465950

Citation

Du, Hai-Ke, et al. "[Effect of Amygdalin On Serum Proteinic Biomarker in Pulmonary Fibrosis of Bleomycin-induced Rat]." Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi = Zhonghua Laodong Weisheng Zhiyebing Zazhi = Chinese Journal of Industrial Hygiene and Occupational Diseases, vol. 28, no. 4, 2010, pp. 260-3.
Du HK, Song FC, Zhou X, et al. [Effect of amygdalin on serum proteinic biomarker in pulmonary fibrosis of bleomycin-induced rat]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2010;28(4):260-3.
Du, H. K., Song, F. C., Zhou, X., Li, H., & Zhang, J. P. (2010). [Effect of amygdalin on serum proteinic biomarker in pulmonary fibrosis of bleomycin-induced rat]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi = Zhonghua Laodong Weisheng Zhiyebing Zazhi = Chinese Journal of Industrial Hygiene and Occupational Diseases, 28(4), pp. 260-3.
Du HK, et al. [Effect of Amygdalin On Serum Proteinic Biomarker in Pulmonary Fibrosis of Bleomycin-induced Rat]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2010;28(4):260-3. PubMed PMID: 20465950.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of amygdalin on serum proteinic biomarker in pulmonary fibrosis of bleomycin-induced rat]. AU - Du,Hai-Ke, AU - Song,Fu-Cheng, AU - Zhou,Xin, AU - Li,He, AU - Zhang,Jian-Peng, PY - 2010/5/15/entrez PY - 2010/5/15/pubmed PY - 2012/10/26/medline SP - 260 EP - 3 JF - Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases JO - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi VL - 28 IS - 4 N2 - OBJECTIVE: To evaluate effect of amygdalin on expression of four biomarkers in the animal model of pulmonary fibrosis induced by bleomycin. METHODS: Rats were given one dose (5 mg/kg) of bleomycin in bleomycin-treated groups, amygdalin-treated groups and saline in controls by intratracheal instillation exposed surgically. The amygdalin-treated groups rats were treated with intraperitoneal injection of amygdalin (15 mg x kg(-1) x day(-1)). The rats were sacrificed 7, 14 and 28 days after bleomycin administration. Polarized light microscopy and Image-Pro Plus detected I and III collagen expressed in Paraffin-embedded lung sections stained with Sirius red. Surface-enhanced laser desorption-ionization time-of-flight mass spectrometry (SELDI-TOF MS) with weak cationic proteinchip (CM10) detected differentially expressed proteins in the pooled serum samples of all groups. RESULTS: Consistent fibrotic responses were found in all bleomycin and amygdalin-tread groups. On the 7th, 14th and 28th day after bleomycin or saline instillation, four differentially expressed proteins were detected in the pooled serum of all groups rats, consisting of 4 proteins with mass/charge ratio of 3530.7, 7043.5, 8332.6 and 9068.0, respectively. Compared with control groups, protein peaks intensity ratio with mass/charge ratio of 3530.7 on 7, 28 d and 7043.5, 8332.6 and 9068.0 on 7, 14 and 28 d was > 2 in bleomycin-treated groups. Compared with amygdalin-treated groups, protein peaks intensity with mass/charge ratio of 3530.7 at 7, 14, 28 d had no change almost, but protein peaks intensity ratio with mass/charge ratio of 7043.5 at 7 d, 8332.6 on 28 d and 9068.0 on 14 d was > 2 in bleomycin-tread groups. All the four protein peaks intensity had no change almost at other point. CONCLUSION: Amygdalin may reduce the bleomycin-induced increase of differentially expressed protein peak intensities in rat serum. SN - 1001-9391 UR - https://www.unboundmedicine.com/medline/citation/20465950/[Effect_of_amygdalin_on_serum_proteinic_biomarker_in_pulmonary_fibrosis_of_bleomycin-induced_rat] L2 - https://medlineplus.gov/pulmonaryfibrosis.html DB - PRIME DP - Unbound Medicine ER -