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pH-sensitive supramolecular polypeptide-based micelles and reverse micelles mediated by hydrogen-bonding interactions or host-guest chemistry: characterization and in vitro controlled drug release.
J Phys Chem B 2010; 114(22):7461-8JP

Abstract

A versatile strategy is provided for the fabrication of pH-sensitive polypeptide-based normal micelles and reverse micelles from the same polypeptide-based copolymers via hydrogen-bonding interactions or host-guest chemistry. The pH-sensitive self-assembly of both linear and dendron-like/linear poly(L-glutamic acid)-b-poly(ethylene oxide) (Dm-PLG-b-PEO) block copolymers was investigated in detail by means of UV-vis, dynamic light scattering, NMR, fluorescence spectroscopy, and transmission electron microscopy. It was demonstrated that both the copolymer topology and the composition controlled the morphology of the polypeptide-cored normal micelles. Importantly, a novel class of polypeptide-shelled reverse micelles was for the first time generated by host-guest-chemistry-mediated self-assembly of these copolymers and alpha-cyclodextrin (alpha-CD) in alkaline solution. The supramolecular inclusion complexation between PEO and alpha-CD was confirmed by wide-angle X-ray diffraction, differential scanning calorimetry, and NMR. Moreover, the zeta potential of the reverse micelles ranged from -20.2 to -24.2 mV, convincingly demonstrating that the reverse micelles had an anionic PLG shell. Furthermore, the anticancer doxorubicin (DOX)-loaded micelles fabricated from the dendron-like/linear copolymer showed a higher DOX loading efficiency (38%) and capacity (24%) and sustained a longer drug-release period (approximately 70 days) than the linear counterpart. Consequently, this will provide a platform for the fabrication of supramolecular polypeptide-cored and polypeptide-shelled micelles for the anticancer drug delivery systems.

Authors+Show Affiliations

Department of Polymer Science & Engineering, School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, PR China.No affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20469900

Citation

Chen, Yi, and Chang-Ming Dong. "PH-sensitive Supramolecular Polypeptide-based Micelles and Reverse Micelles Mediated By Hydrogen-bonding Interactions or Host-guest Chemistry: Characterization and in Vitro Controlled Drug Release." The Journal of Physical Chemistry. B, vol. 114, no. 22, 2010, pp. 7461-8.
Chen Y, Dong CM. PH-sensitive supramolecular polypeptide-based micelles and reverse micelles mediated by hydrogen-bonding interactions or host-guest chemistry: characterization and in vitro controlled drug release. J Phys Chem B. 2010;114(22):7461-8.
Chen, Y., & Dong, C. M. (2010). PH-sensitive supramolecular polypeptide-based micelles and reverse micelles mediated by hydrogen-bonding interactions or host-guest chemistry: characterization and in vitro controlled drug release. The Journal of Physical Chemistry. B, 114(22), pp. 7461-8. doi:10.1021/jp100399d.
Chen Y, Dong CM. PH-sensitive Supramolecular Polypeptide-based Micelles and Reverse Micelles Mediated By Hydrogen-bonding Interactions or Host-guest Chemistry: Characterization and in Vitro Controlled Drug Release. J Phys Chem B. 2010 Jun 10;114(22):7461-8. PubMed PMID: 20469900.
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TY - JOUR T1 - pH-sensitive supramolecular polypeptide-based micelles and reverse micelles mediated by hydrogen-bonding interactions or host-guest chemistry: characterization and in vitro controlled drug release. AU - Chen,Yi, AU - Dong,Chang-Ming, PY - 2010/5/18/entrez PY - 2010/5/18/pubmed PY - 2010/9/10/medline SP - 7461 EP - 8 JF - The journal of physical chemistry. B JO - J Phys Chem B VL - 114 IS - 22 N2 - A versatile strategy is provided for the fabrication of pH-sensitive polypeptide-based normal micelles and reverse micelles from the same polypeptide-based copolymers via hydrogen-bonding interactions or host-guest chemistry. The pH-sensitive self-assembly of both linear and dendron-like/linear poly(L-glutamic acid)-b-poly(ethylene oxide) (Dm-PLG-b-PEO) block copolymers was investigated in detail by means of UV-vis, dynamic light scattering, NMR, fluorescence spectroscopy, and transmission electron microscopy. It was demonstrated that both the copolymer topology and the composition controlled the morphology of the polypeptide-cored normal micelles. Importantly, a novel class of polypeptide-shelled reverse micelles was for the first time generated by host-guest-chemistry-mediated self-assembly of these copolymers and alpha-cyclodextrin (alpha-CD) in alkaline solution. The supramolecular inclusion complexation between PEO and alpha-CD was confirmed by wide-angle X-ray diffraction, differential scanning calorimetry, and NMR. Moreover, the zeta potential of the reverse micelles ranged from -20.2 to -24.2 mV, convincingly demonstrating that the reverse micelles had an anionic PLG shell. Furthermore, the anticancer doxorubicin (DOX)-loaded micelles fabricated from the dendron-like/linear copolymer showed a higher DOX loading efficiency (38%) and capacity (24%) and sustained a longer drug-release period (approximately 70 days) than the linear counterpart. Consequently, this will provide a platform for the fabrication of supramolecular polypeptide-cored and polypeptide-shelled micelles for the anticancer drug delivery systems. SN - 1520-5207 UR - https://www.unboundmedicine.com/medline/citation/20469900/pH_sensitive_supramolecular_polypeptide_based_micelles_and_reverse_micelles_mediated_by_hydrogen_bonding_interactions_or_host_guest_chemistry:_characterization_and_in_vitro_controlled_drug_release_ L2 - https://dx.doi.org/10.1021/jp100399d DB - PRIME DP - Unbound Medicine ER -