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Entry exclusion and oriT of a conjugative system encoded by the cryptic plasmid p29930 of Yersinia enterocolitica.
Plasmid 2010; 64(2):79-84P

Abstract

The conjugative transfer system of Yersinia enterocolitica 29930 present on the cryptic plasmid p29930 comprises a mating pore formation system (Mpf) related to that of the IncX plasmid R6K and a DNA transfer and replication system (Dtr) with close relationship to the mob region of the mobilizable plasmid CloDF13. Two regions of the transfer system were selected for more detailed analyses of basic functions of conjugative transfer. The putative open reading frame orf22 located in the Mpf region confers the entry exclusion phenotype to possible recipient cells and inhibited conjugative transfer, when it was inserted into the coding region of the cat gene of pACYC184 in sense direction. Mobilization experiments with recombinant plasmids revealed that a 611bp fragment of the Dtr region containing two repeat sequences were required for a functional oriT by the conjugation system of Y. enterocolitica. While the conjugative transfer of cryptic plasmids harbouring the complete conjugation system had not been demonstrated previously, plasmid pBK17 containing the functional oriT was successfully mobilized from Y. enterocolitica strains into Escherichia coli, thus proving that the transfer system could contribute to the spread of these plasmids in nature.

Authors+Show Affiliations

Department of Biological Safety, Bundesinstitut für Risikobewertung, Federal Institute for Risk Assessment, D-12277 Berlin, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20470820

Citation

Kraushaar, Britta, et al. "Entry Exclusion and oriT of a Conjugative System Encoded By the Cryptic Plasmid P29930 of Yersinia Enterocolitica." Plasmid, vol. 64, no. 2, 2010, pp. 79-84.
Kraushaar B, Appel B, Lanka E, et al. Entry exclusion and oriT of a conjugative system encoded by the cryptic plasmid p29930 of Yersinia enterocolitica. Plasmid. 2010;64(2):79-84.
Kraushaar, B., Appel, B., Lanka, E., & Strauch, E. (2010). Entry exclusion and oriT of a conjugative system encoded by the cryptic plasmid p29930 of Yersinia enterocolitica. Plasmid, 64(2), pp. 79-84. doi:10.1016/j.plasmid.2010.05.001.
Kraushaar B, et al. Entry Exclusion and oriT of a Conjugative System Encoded By the Cryptic Plasmid P29930 of Yersinia Enterocolitica. Plasmid. 2010;64(2):79-84. PubMed PMID: 20470820.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Entry exclusion and oriT of a conjugative system encoded by the cryptic plasmid p29930 of Yersinia enterocolitica. AU - Kraushaar,Britta, AU - Appel,Bernd, AU - Lanka,Erich, AU - Strauch,Eckhard, Y1 - 2010/05/12/ PY - 2010/02/26/received PY - 2010/05/05/revised PY - 2010/05/07/accepted PY - 2010/5/18/entrez PY - 2010/5/18/pubmed PY - 2010/10/16/medline SP - 79 EP - 84 JF - Plasmid JO - Plasmid VL - 64 IS - 2 N2 - The conjugative transfer system of Yersinia enterocolitica 29930 present on the cryptic plasmid p29930 comprises a mating pore formation system (Mpf) related to that of the IncX plasmid R6K and a DNA transfer and replication system (Dtr) with close relationship to the mob region of the mobilizable plasmid CloDF13. Two regions of the transfer system were selected for more detailed analyses of basic functions of conjugative transfer. The putative open reading frame orf22 located in the Mpf region confers the entry exclusion phenotype to possible recipient cells and inhibited conjugative transfer, when it was inserted into the coding region of the cat gene of pACYC184 in sense direction. Mobilization experiments with recombinant plasmids revealed that a 611bp fragment of the Dtr region containing two repeat sequences were required for a functional oriT by the conjugation system of Y. enterocolitica. While the conjugative transfer of cryptic plasmids harbouring the complete conjugation system had not been demonstrated previously, plasmid pBK17 containing the functional oriT was successfully mobilized from Y. enterocolitica strains into Escherichia coli, thus proving that the transfer system could contribute to the spread of these plasmids in nature. SN - 1095-9890 UR - https://www.unboundmedicine.com/medline/citation/20470820/Entry_exclusion_and_oriT_of_a_conjugative_system_encoded_by_the_cryptic_plasmid_p29930_of_Yersinia_enterocolitica_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-619X(10)00033-8 DB - PRIME DP - Unbound Medicine ER -