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Protective effects of Gastrodia elata Blume on MPP+-induced cytotoxicity in human dopaminergic SH-SY5Y cells.
J Ethnopharmacol. 2010 Jul 20; 130(2):290-8.JE

Abstract

AIM OF THE STUDY

Gastrodia elata (GE) Blume (Orchidaceae) has been traditionally used as a folk medicine in Oriental countries since centuries for their variety of therapeutic benefits. This study is an attempt to investigate the protective effects of GE extract against MPP(+)-induced cytotoxicity in human dopaminergic SH-SY5Y cells and explore the neuroprotective mechanisms involved.

MATERIALS AND METHODS

Human dopaminergic SH-SY5Y cells were used to demonstrate the protective effects of GE against multiple parameters such as MPP(+)-induced cell viability, oxidative damage, expression of Bcl-2 and Bax, caspase-3 and poly(ADP-ribose) polymerase proteolysis.

RESULTS

GE effectively attenuated the cytotoxicity and improved cell viability in a dose-dependent manner. GE was effective in inhibiting both, the increased production of reactive oxygen species (ROS) and increase in Bax/Bcl-2 ratio, cleaved caspase-3 and PARP proteolysis.

CONCLUSION

Data from this study suggests the protective effects of GE on MPP(+)-induced cytotoxicity in dopaminergic cells, which may be ascribed to its significant anti-oxidative and anti-apoptotic properties, thus, GE might prove to be a valuable therapeutic agent for the treatment of various neurodegenerative diseases including progressive Parkinson's disease (PD).

Authors+Show Affiliations

Department of Biotechnology, Konkuk University, Chungju, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20470875

Citation

An, Hua, et al. "Protective Effects of Gastrodia Elata Blume On MPP+-induced Cytotoxicity in Human Dopaminergic SH-SY5Y Cells." Journal of Ethnopharmacology, vol. 130, no. 2, 2010, pp. 290-8.
An H, Kim IS, Koppula S, et al. Protective effects of Gastrodia elata Blume on MPP+-induced cytotoxicity in human dopaminergic SH-SY5Y cells. J Ethnopharmacol. 2010;130(2):290-8.
An, H., Kim, I. S., Koppula, S., Kim, B. W., Park, P. J., Lim, B. O., Choi, W. S., Lee, K. H., & Choi, D. K. (2010). Protective effects of Gastrodia elata Blume on MPP+-induced cytotoxicity in human dopaminergic SH-SY5Y cells. Journal of Ethnopharmacology, 130(2), 290-8. https://doi.org/10.1016/j.jep.2010.05.006
An H, et al. Protective Effects of Gastrodia Elata Blume On MPP+-induced Cytotoxicity in Human Dopaminergic SH-SY5Y Cells. J Ethnopharmacol. 2010 Jul 20;130(2):290-8. PubMed PMID: 20470875.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of Gastrodia elata Blume on MPP+-induced cytotoxicity in human dopaminergic SH-SY5Y cells. AU - An,Hua, AU - Kim,In Su, AU - Koppula,Sushruta, AU - Kim,Byung Wook, AU - Park,Pyo Jam, AU - Lim,Beong Ou, AU - Choi,Wahn Soo, AU - Lee,Kwang Ho, AU - Choi,Dong Kug, Y1 - 2010/05/12/ PY - 2010/03/24/received PY - 2010/04/26/revised PY - 2010/05/05/accepted PY - 2010/5/18/entrez PY - 2010/5/18/pubmed PY - 2010/11/5/medline SP - 290 EP - 8 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 130 IS - 2 N2 - AIM OF THE STUDY: Gastrodia elata (GE) Blume (Orchidaceae) has been traditionally used as a folk medicine in Oriental countries since centuries for their variety of therapeutic benefits. This study is an attempt to investigate the protective effects of GE extract against MPP(+)-induced cytotoxicity in human dopaminergic SH-SY5Y cells and explore the neuroprotective mechanisms involved. MATERIALS AND METHODS: Human dopaminergic SH-SY5Y cells were used to demonstrate the protective effects of GE against multiple parameters such as MPP(+)-induced cell viability, oxidative damage, expression of Bcl-2 and Bax, caspase-3 and poly(ADP-ribose) polymerase proteolysis. RESULTS: GE effectively attenuated the cytotoxicity and improved cell viability in a dose-dependent manner. GE was effective in inhibiting both, the increased production of reactive oxygen species (ROS) and increase in Bax/Bcl-2 ratio, cleaved caspase-3 and PARP proteolysis. CONCLUSION: Data from this study suggests the protective effects of GE on MPP(+)-induced cytotoxicity in dopaminergic cells, which may be ascribed to its significant anti-oxidative and anti-apoptotic properties, thus, GE might prove to be a valuable therapeutic agent for the treatment of various neurodegenerative diseases including progressive Parkinson's disease (PD). SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/20470875/Protective_effects_of_Gastrodia_elata_Blume_on_MPP+_induced_cytotoxicity_in_human_dopaminergic_SH_SY5Y_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(10)00306-5 DB - PRIME DP - Unbound Medicine ER -