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Cannabidiol induced a contrasting pro-apoptotic effect between freshly isolated and precultured human monocytes.
Toxicol Appl Pharmacol 2010; 246(3):141-7TA

Abstract

It has been documented that cannabidiol (CBD) induced apoptosis in a variety of transformed cells, including lymphocytic and monocytic leukemias. In contrast, a differential sensitivity between normal lymphocytes and monocytes to CBD-mediated apoptosis has been reported. The present study investigated the pro-apoptotic effect of CBD on human peripheral monocytes that were either freshly isolated or precultured for 72h. CBD markedly enhanced apoptosis of freshly isolated monocytes in a time- and concentration-dependent manner, whereas precultured monocytes were insensitive. By comparison, both cells were sensitive to doxorubicin-induced apoptosis. CBD significantly diminished the cellular thiols and glutathione in freshly isolated monocytes. The apoptosis induced by CBD was abrogated in the presence of N-acetyl-L-cysteine, a precursor of glutathione. In addition, precultured monocytes contained a significantly greater level of glutathione and heme oxygenase-1 (HO-1) compared to the freshly isolated cells. The HO-1 competitive inhibitor zinc protoporphyrin partially but significantly restored the sensitivity of precultured monocytes to CBD-mediated apoptosis. Collectively, our results demonstrated a contrasting pro-apoptotic effect of CBD between precultured and freshly isolated monocytes, which was closely associated with the cellular level of glutathione and the antioxidative capability of the cells.

Authors+Show Affiliations

Department and Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.Department and Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.Department and Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.Department and Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.Department and Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.Graduate Institute of Biomedical Materials and Engineering, Taipei Medical University, Taipei, Taiwan.Department and Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20471992

Citation

Wu, Hsin-Ying, et al. "Cannabidiol Induced a Contrasting Pro-apoptotic Effect Between Freshly Isolated and Precultured Human Monocytes." Toxicology and Applied Pharmacology, vol. 246, no. 3, 2010, pp. 141-7.
Wu HY, Chang AC, Wang CC, et al. Cannabidiol induced a contrasting pro-apoptotic effect between freshly isolated and precultured human monocytes. Toxicol Appl Pharmacol. 2010;246(3):141-7.
Wu, H. Y., Chang, A. C., Wang, C. C., Kuo, F. H., Lee, C. Y., Liu, D. Z., & Jan, T. R. (2010). Cannabidiol induced a contrasting pro-apoptotic effect between freshly isolated and precultured human monocytes. Toxicology and Applied Pharmacology, 246(3), pp. 141-7. doi:10.1016/j.taap.2010.05.003.
Wu HY, et al. Cannabidiol Induced a Contrasting Pro-apoptotic Effect Between Freshly Isolated and Precultured Human Monocytes. Toxicol Appl Pharmacol. 2010 Aug 1;246(3):141-7. PubMed PMID: 20471992.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cannabidiol induced a contrasting pro-apoptotic effect between freshly isolated and precultured human monocytes. AU - Wu,Hsin-Ying, AU - Chang,An-Chi, AU - Wang,Chia-Chi, AU - Kuo,Fu-Hua, AU - Lee,Chi-Ya, AU - Liu,Der-Zen, AU - Jan,Tong-Rong, Y1 - 2010/05/21/ PY - 2010/01/22/received PY - 2010/04/28/revised PY - 2010/05/07/accepted PY - 2010/5/18/entrez PY - 2010/5/18/pubmed PY - 2014/8/8/medline SP - 141 EP - 7 JF - Toxicology and applied pharmacology JO - Toxicol. Appl. Pharmacol. VL - 246 IS - 3 N2 - It has been documented that cannabidiol (CBD) induced apoptosis in a variety of transformed cells, including lymphocytic and monocytic leukemias. In contrast, a differential sensitivity between normal lymphocytes and monocytes to CBD-mediated apoptosis has been reported. The present study investigated the pro-apoptotic effect of CBD on human peripheral monocytes that were either freshly isolated or precultured for 72h. CBD markedly enhanced apoptosis of freshly isolated monocytes in a time- and concentration-dependent manner, whereas precultured monocytes were insensitive. By comparison, both cells were sensitive to doxorubicin-induced apoptosis. CBD significantly diminished the cellular thiols and glutathione in freshly isolated monocytes. The apoptosis induced by CBD was abrogated in the presence of N-acetyl-L-cysteine, a precursor of glutathione. In addition, precultured monocytes contained a significantly greater level of glutathione and heme oxygenase-1 (HO-1) compared to the freshly isolated cells. The HO-1 competitive inhibitor zinc protoporphyrin partially but significantly restored the sensitivity of precultured monocytes to CBD-mediated apoptosis. Collectively, our results demonstrated a contrasting pro-apoptotic effect of CBD between precultured and freshly isolated monocytes, which was closely associated with the cellular level of glutathione and the antioxidative capability of the cells. SN - 1096-0333 UR - https://www.unboundmedicine.com/medline/citation/20471992/abstract/Cannabidiol_induced_a_contrasting_pro_apoptotic_effect_between_freshly_isolated_and_precultured_human_monocytes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-008X(10)00167-5 DB - PRIME DP - Unbound Medicine ER -