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Interactions of SARS coronavirus nucleocapsid protein with the host cell proteasome subunit p42.
Virol J. 2010 May 17; 7:99.VJ

Abstract

BACKGROUND

Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) spreads rapidly and has a high case-mortality rate. The nucleocapsid protein (NP) of SARS-CoV may be critical for pathogenicity. This study sought to discover the host proteins that interact with SARS-CoV NP.

RESULTS

Using surface plasmon resonance biomolecular interaction analysis (SPR/BIA) and matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry, we found that only the proteasome subunit p42 from human fetal lung diploid fibroblast (2BS) cells bound to SARS-CoV NP. This interaction was confirmed by the glutathione S-transferase (GST) fusion protein pulldown technique. The co-localization signal of SARS-CoV NP and proteasome subunit p42 in 2BS cells was detected using indirect immunofluorescence and confocal microscopy. p42 is a subunit of the 26S proteasome; this large, multi-protein complex is a component of the ubiquitin-proteasome pathway, which is involved in a variety of basic cellular processes and inflammatory responses.

CONCLUSION

To our knowledge, this is the first report that SARS-CoV NP interacts with the proteasome subunit p42 within host cells. These data enhance our understanding of the molecular mechanisms of SARS-CoV pathogenicity and the means by which SARS-CoV interacts with host cells.

Authors+Show Affiliations

State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, China CDC 100 Ying Xin Jie, Xuan Wu Qu, Beijing 100052, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20478047

Citation

Wang, Qin, et al. "Interactions of SARS Coronavirus Nucleocapsid Protein With the Host Cell Proteasome Subunit P42." Virology Journal, vol. 7, 2010, p. 99.
Wang Q, Li C, Zhang Q, et al. Interactions of SARS coronavirus nucleocapsid protein with the host cell proteasome subunit p42. Virol J. 2010;7:99.
Wang, Q., Li, C., Zhang, Q., Wang, T., Li, J., Guan, W., Yu, J., Liang, M., & Li, D. (2010). Interactions of SARS coronavirus nucleocapsid protein with the host cell proteasome subunit p42. Virology Journal, 7, 99. https://doi.org/10.1186/1743-422X-7-99
Wang Q, et al. Interactions of SARS Coronavirus Nucleocapsid Protein With the Host Cell Proteasome Subunit P42. Virol J. 2010 May 17;7:99. PubMed PMID: 20478047.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interactions of SARS coronavirus nucleocapsid protein with the host cell proteasome subunit p42. AU - Wang,Qin, AU - Li,Chuan, AU - Zhang,Quanfu, AU - Wang,Tao, AU - Li,Jiandong, AU - Guan,Wuxiang, AU - Yu,Jianshi, AU - Liang,Mifang, AU - Li,Dexin, Y1 - 2010/05/17/ PY - 2010/01/21/received PY - 2010/05/17/accepted PY - 2010/5/19/entrez PY - 2010/5/19/pubmed PY - 2010/9/8/medline SP - 99 EP - 99 JF - Virology journal JO - Virol. J. VL - 7 N2 - BACKGROUND: Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) spreads rapidly and has a high case-mortality rate. The nucleocapsid protein (NP) of SARS-CoV may be critical for pathogenicity. This study sought to discover the host proteins that interact with SARS-CoV NP. RESULTS: Using surface plasmon resonance biomolecular interaction analysis (SPR/BIA) and matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry, we found that only the proteasome subunit p42 from human fetal lung diploid fibroblast (2BS) cells bound to SARS-CoV NP. This interaction was confirmed by the glutathione S-transferase (GST) fusion protein pulldown technique. The co-localization signal of SARS-CoV NP and proteasome subunit p42 in 2BS cells was detected using indirect immunofluorescence and confocal microscopy. p42 is a subunit of the 26S proteasome; this large, multi-protein complex is a component of the ubiquitin-proteasome pathway, which is involved in a variety of basic cellular processes and inflammatory responses. CONCLUSION: To our knowledge, this is the first report that SARS-CoV NP interacts with the proteasome subunit p42 within host cells. These data enhance our understanding of the molecular mechanisms of SARS-CoV pathogenicity and the means by which SARS-CoV interacts with host cells. SN - 1743-422X UR - https://www.unboundmedicine.com/medline/citation/20478047/Interactions_of_SARS_coronavirus_nucleocapsid_protein_with_the_host_cell_proteasome_subunit_p42_ L2 - https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-7-99 DB - PRIME DP - Unbound Medicine ER -