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Estrogen regulation of TRPM8 expression in breast cancer cells.
BMC Cancer. 2010 May 19; 10:212.BC

Abstract

BACKGROUND

The calcium-permeable cation channel TRPM8 (melastatin-related transient receptor potential member 8) is over-expressed in several cancers. The present study aimed at investigating the expression, function and potential regulation of TRPM8 channels by ER alpha (estrogen receptor alpha) in breast cancer.

METHODS

RT-PCR, Western blot, immuno-histochemical, and siRNA techniques were used to investigate TRPM8 expression, its regulation by estrogen receptors, and its expression in breast tissue. To investigate the channel activity in MCF-7 cells, we used the whole cell patch clamp and the calcium imaging techniques.

RESULTS

TRPM8 channels are expressed at both mRNA and protein levels in the breast cancer cell line MCF-7. Bath application of the potent TRPM8 agonist Icilin (20 microM) induced a strong outwardly rectifying current at depolarizing potentials, which is associated with an elevation of cytosolic calcium concentration, consistent with established TRPM8 channel properties. RT-PCR experiments revealed a decrease in TRPM8 mRNA expression following steroid deprivation for 48 and 72 hours. In steroid deprived medium, addition of 17-beta-estradiol (E2, 10 nM) increased both TRPM8 mRNA expression and the number of cells which respond to Icilin, but failed to affect the Ca2+ entry amplitude. Moreover, silencing ERalpha mRNA expression with small interfering RNA reduced the expression of TRPM8. Immuno-histochemical examination of the expression of TRPM8 channels in human breast tissues revealed an over-expression of TRPM8 in breast adenocarcinomas, which is correlated with estrogen receptor positive (ER+) status of the tumours.

CONCLUSION

Taken together, these results show that TRPM8 channels are expressed and functional in breast cancer and that their expression is regulated by ER alpha.

Authors+Show Affiliations

Laboratoire de Physiologie Cellulaire et Moléculaire, JE 2530: Canaux ioniques dans cancer du sein, Faculté des Sciences, Université Picardie Jules Vernes, 33 rue Saint Leu, 80000, Amiens, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20482834

Citation

Chodon, Dechen, et al. "Estrogen Regulation of TRPM8 Expression in Breast Cancer Cells." BMC Cancer, vol. 10, 2010, p. 212.
Chodon D, Guilbert A, Dhennin-Duthille I, et al. Estrogen regulation of TRPM8 expression in breast cancer cells. BMC Cancer. 2010;10:212.
Chodon, D., Guilbert, A., Dhennin-Duthille, I., Gautier, M., Telliez, M. S., Sevestre, H., & Ouadid-Ahidouch, H. (2010). Estrogen regulation of TRPM8 expression in breast cancer cells. BMC Cancer, 10, 212. https://doi.org/10.1186/1471-2407-10-212
Chodon D, et al. Estrogen Regulation of TRPM8 Expression in Breast Cancer Cells. BMC Cancer. 2010 May 19;10:212. PubMed PMID: 20482834.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Estrogen regulation of TRPM8 expression in breast cancer cells. AU - Chodon,Dechen, AU - Guilbert,Arnaud, AU - Dhennin-Duthille,Isabelle, AU - Gautier,Mathieu, AU - Telliez,Marie-Sophie, AU - Sevestre,Henri, AU - Ouadid-Ahidouch,Halima, Y1 - 2010/05/19/ PY - 2009/10/26/received PY - 2010/05/19/accepted PY - 2010/5/21/entrez PY - 2010/5/21/pubmed PY - 2010/8/17/medline SP - 212 EP - 212 JF - BMC cancer JO - BMC Cancer VL - 10 N2 - BACKGROUND: The calcium-permeable cation channel TRPM8 (melastatin-related transient receptor potential member 8) is over-expressed in several cancers. The present study aimed at investigating the expression, function and potential regulation of TRPM8 channels by ER alpha (estrogen receptor alpha) in breast cancer. METHODS: RT-PCR, Western blot, immuno-histochemical, and siRNA techniques were used to investigate TRPM8 expression, its regulation by estrogen receptors, and its expression in breast tissue. To investigate the channel activity in MCF-7 cells, we used the whole cell patch clamp and the calcium imaging techniques. RESULTS: TRPM8 channels are expressed at both mRNA and protein levels in the breast cancer cell line MCF-7. Bath application of the potent TRPM8 agonist Icilin (20 microM) induced a strong outwardly rectifying current at depolarizing potentials, which is associated with an elevation of cytosolic calcium concentration, consistent with established TRPM8 channel properties. RT-PCR experiments revealed a decrease in TRPM8 mRNA expression following steroid deprivation for 48 and 72 hours. In steroid deprived medium, addition of 17-beta-estradiol (E2, 10 nM) increased both TRPM8 mRNA expression and the number of cells which respond to Icilin, but failed to affect the Ca2+ entry amplitude. Moreover, silencing ERalpha mRNA expression with small interfering RNA reduced the expression of TRPM8. Immuno-histochemical examination of the expression of TRPM8 channels in human breast tissues revealed an over-expression of TRPM8 in breast adenocarcinomas, which is correlated with estrogen receptor positive (ER+) status of the tumours. CONCLUSION: Taken together, these results show that TRPM8 channels are expressed and functional in breast cancer and that their expression is regulated by ER alpha. SN - 1471-2407 UR - https://www.unboundmedicine.com/medline/citation/20482834/Estrogen_regulation_of_TRPM8_expression_in_breast_cancer_cells_ L2 - https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-10-212 DB - PRIME DP - Unbound Medicine ER -