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Viral reassortment and transmission after co-infection of pigs with classical H1N1 and triple-reassortant H3N2 swine influenza viruses.
J Gen Virol 2010; 91(Pt 9):2314-21JG

Abstract

Triple-reassortant swine influenza viruses circulating in North American pigs contain the internal genes derived from swine (matrix, non-structural and nucleoprotein), human [polymerase basic 1 (PB1)] and avian (polymerase acidic and PB2) influenza viruses forming a constellation of genes that is well conserved and is called the triple-reassortant internal gene (TRIG) cassette. In contrast, the external genes [haemagglutinin (HA) and neuraminidase (NA)] are less conserved, reflecting multiple reassortant events that have produced viruses with different combinations of HA and NA genes. This study hypothesized that maintenance of the TRIG cassette confers a selective advantage to the virus. To test this hypothesis, pigs were co-infected with the triple-reassortant H3N2 A/Swine/Texas/4199-2/98 (Tx/98) and the classical H1N1 A/Swine/Iowa/15/1930 viruses and co-housed with a group of sentinel animals. This direct contact group was subsequently moved into contact with a second group of naïve animals. Four different subtypes (H1N1, H1N2, H3N1 and H3N2) of influenza virus were identified in bronchoalveolar lavage fluid collected from the lungs of the experimentally infected pigs, with most of the viruses containing TRIG from the Tx/98 virus. Interestingly, only the intact H3N2 Tx/98 virus was transmitted from the infected pigs to the direct-contact animals and from them to the second contact group of pigs. These results demonstrated that multiple reassortments can occur within a host; however, only specific gene constellations are readily transmissible. It was concluded that certain HA and NA gene pairs, in conjunction with the TRIG cassette, may have a competitive advantage over other combinations for transmission and maintenance in swine.

Authors+Show Affiliations

Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, IA, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

20484565

Citation

Ma, Wenjun, et al. "Viral Reassortment and Transmission After Co-infection of Pigs With Classical H1N1 and Triple-reassortant H3N2 Swine Influenza Viruses." The Journal of General Virology, vol. 91, no. Pt 9, 2010, pp. 2314-21.
Ma W, Lager KM, Lekcharoensuk P, et al. Viral reassortment and transmission after co-infection of pigs with classical H1N1 and triple-reassortant H3N2 swine influenza viruses. J Gen Virol. 2010;91(Pt 9):2314-21.
Ma, W., Lager, K. M., Lekcharoensuk, P., Ulery, E. S., Janke, B. H., Solórzano, A., ... Richt, J. A. (2010). Viral reassortment and transmission after co-infection of pigs with classical H1N1 and triple-reassortant H3N2 swine influenza viruses. The Journal of General Virology, 91(Pt 9), pp. 2314-21. doi:10.1099/vir.0.021402-0.
Ma W, et al. Viral Reassortment and Transmission After Co-infection of Pigs With Classical H1N1 and Triple-reassortant H3N2 Swine Influenza Viruses. J Gen Virol. 2010;91(Pt 9):2314-21. PubMed PMID: 20484565.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Viral reassortment and transmission after co-infection of pigs with classical H1N1 and triple-reassortant H3N2 swine influenza viruses. AU - Ma,Wenjun, AU - Lager,Kelly M, AU - Lekcharoensuk,Porntippa, AU - Ulery,Eva S, AU - Janke,Bruce H, AU - Solórzano,Alicia, AU - Webby,Richard J, AU - García-Sastre,Adolfo, AU - Richt,Jürgen A, Y1 - 2010/05/19/ PY - 2010/5/21/entrez PY - 2010/5/21/pubmed PY - 2010/9/14/medline SP - 2314 EP - 21 JF - The Journal of general virology JO - J. Gen. Virol. VL - 91 IS - Pt 9 N2 - Triple-reassortant swine influenza viruses circulating in North American pigs contain the internal genes derived from swine (matrix, non-structural and nucleoprotein), human [polymerase basic 1 (PB1)] and avian (polymerase acidic and PB2) influenza viruses forming a constellation of genes that is well conserved and is called the triple-reassortant internal gene (TRIG) cassette. In contrast, the external genes [haemagglutinin (HA) and neuraminidase (NA)] are less conserved, reflecting multiple reassortant events that have produced viruses with different combinations of HA and NA genes. This study hypothesized that maintenance of the TRIG cassette confers a selective advantage to the virus. To test this hypothesis, pigs were co-infected with the triple-reassortant H3N2 A/Swine/Texas/4199-2/98 (Tx/98) and the classical H1N1 A/Swine/Iowa/15/1930 viruses and co-housed with a group of sentinel animals. This direct contact group was subsequently moved into contact with a second group of naïve animals. Four different subtypes (H1N1, H1N2, H3N1 and H3N2) of influenza virus were identified in bronchoalveolar lavage fluid collected from the lungs of the experimentally infected pigs, with most of the viruses containing TRIG from the Tx/98 virus. Interestingly, only the intact H3N2 Tx/98 virus was transmitted from the infected pigs to the direct-contact animals and from them to the second contact group of pigs. These results demonstrated that multiple reassortments can occur within a host; however, only specific gene constellations are readily transmissible. It was concluded that certain HA and NA gene pairs, in conjunction with the TRIG cassette, may have a competitive advantage over other combinations for transmission and maintenance in swine. SN - 1465-2099 UR - https://www.unboundmedicine.com/medline/citation/20484565/Viral_reassortment_and_transmission_after_co_infection_of_pigs_with_classical_H1N1_and_triple_reassortant_H3N2_swine_influenza_viruses_ L2 - http://jgv.microbiologyresearch.org/pubmed/content/journal/jgv/10.1099/vir.0.021402-0 DB - PRIME DP - Unbound Medicine ER -