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Anti-inflammatory effect of catechin on cultured human dental pulp cells affected by bacteria-derived factors.

Abstract

Catechins (bioactive polyphenols in green tea) are known to exhibit potent anti-inflammatory properties. However, the anti-inflammatory effects of catechins on inflamed dental pulp tissue are not known. In this study, we investigated the effect of epigallocatechin-3-gallate (EGCG) and epicatechin gallate (ECG), the major components of green tea catechins, on the expression of pro-inflammatory cytokines and adhesion molecules in human dental pulp cells stimulated with bacteria-derived factors such as lipopolysaccharide (LPS) and peptidoglycan (PG). The expression of interleukin (IL)-6 and of IL-8 was examined using the reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assays. The expression of intercellular adhesion molecule-1 (ICAM-1) and of vascular cell adhesion molecule-1 (VCAM-1) on dental pulp cells was analyzed using flow cytometry. The presence of EGCG and ECG significantly reduced, in a concentration-dependent manner, the expression of IL-6 and IL-8 in dental pulp cells exposed to LPS or PG. Increased expression of ICAM-1 and VCAM-1 on the dental pulp cells in response to bacterial components was also decreased by treatment with EGCG and ECG. These findings suggest that green tea catechins may prevent the exacerbation of pulpitis.

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  • Authors+Show Affiliations

    ,

    Department of Conservative Dentistry, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan. tadashi@dent.tokushima-u.ac.jp

    , , , ,

    Source

    European journal of oral sciences 118:2 2010 Apr pg 145-50

    MeSH

    Anti-Inflammatory Agents
    Antioxidants
    Catechin
    Cells, Cultured
    Dental Pulp
    Escherichia coli
    Flow Cytometry
    Humans
    Intercellular Adhesion Molecule-1
    Interleukin-6
    Interleukin-8
    Lipopolysaccharides
    Peptidoglycan
    Reverse Transcriptase Polymerase Chain Reaction
    Staphylococcus aureus
    Time Factors
    Vascular Cell Adhesion Molecule-1

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    20487003

    Citation

    Nakanishi, Tadashi, et al. "Anti-inflammatory Effect of Catechin On Cultured Human Dental Pulp Cells Affected By Bacteria-derived Factors." European Journal of Oral Sciences, vol. 118, no. 2, 2010, pp. 145-50.
    Nakanishi T, Mukai K, Yumoto H, et al. Anti-inflammatory effect of catechin on cultured human dental pulp cells affected by bacteria-derived factors. Eur J Oral Sci. 2010;118(2):145-50.
    Nakanishi, T., Mukai, K., Yumoto, H., Hirao, K., Hosokawa, Y., & Matsuo, T. (2010). Anti-inflammatory effect of catechin on cultured human dental pulp cells affected by bacteria-derived factors. European Journal of Oral Sciences, 118(2), pp. 145-50. doi:10.1111/j.1600-0722.2010.00714.x.
    Nakanishi T, et al. Anti-inflammatory Effect of Catechin On Cultured Human Dental Pulp Cells Affected By Bacteria-derived Factors. Eur J Oral Sci. 2010;118(2):145-50. PubMed PMID: 20487003.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Anti-inflammatory effect of catechin on cultured human dental pulp cells affected by bacteria-derived factors. AU - Nakanishi,Tadashi, AU - Mukai,Kayo, AU - Yumoto,Hiromichi, AU - Hirao,Kouji, AU - Hosokawa,Yoshitaka, AU - Matsuo,Takashi, PY - 2010/5/22/entrez PY - 2010/5/22/pubmed PY - 2010/9/18/medline SP - 145 EP - 50 JF - European journal of oral sciences JO - Eur. J. Oral Sci. VL - 118 IS - 2 N2 - Catechins (bioactive polyphenols in green tea) are known to exhibit potent anti-inflammatory properties. However, the anti-inflammatory effects of catechins on inflamed dental pulp tissue are not known. In this study, we investigated the effect of epigallocatechin-3-gallate (EGCG) and epicatechin gallate (ECG), the major components of green tea catechins, on the expression of pro-inflammatory cytokines and adhesion molecules in human dental pulp cells stimulated with bacteria-derived factors such as lipopolysaccharide (LPS) and peptidoglycan (PG). The expression of interleukin (IL)-6 and of IL-8 was examined using the reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assays. The expression of intercellular adhesion molecule-1 (ICAM-1) and of vascular cell adhesion molecule-1 (VCAM-1) on dental pulp cells was analyzed using flow cytometry. The presence of EGCG and ECG significantly reduced, in a concentration-dependent manner, the expression of IL-6 and IL-8 in dental pulp cells exposed to LPS or PG. Increased expression of ICAM-1 and VCAM-1 on the dental pulp cells in response to bacterial components was also decreased by treatment with EGCG and ECG. These findings suggest that green tea catechins may prevent the exacerbation of pulpitis. SN - 1600-0722 UR - https://www.unboundmedicine.com/medline/citation/20487003/Anti_inflammatory_effect_of_catechin_on_cultured_human_dental_pulp_cells_affected_by_bacteria_derived_factors_ L2 - https://doi.org/10.1111/j.1600-0722.2010.00714.x DB - PRIME DP - Unbound Medicine ER -