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Anti-inflammatory effect of catechin on cultured human dental pulp cells affected by bacteria-derived factors.
Eur J Oral Sci 2010; 118(2):145-50EJ

Abstract

Catechins (bioactive polyphenols in green tea) are known to exhibit potent anti-inflammatory properties. However, the anti-inflammatory effects of catechins on inflamed dental pulp tissue are not known. In this study, we investigated the effect of epigallocatechin-3-gallate (EGCG) and epicatechin gallate (ECG), the major components of green tea catechins, on the expression of pro-inflammatory cytokines and adhesion molecules in human dental pulp cells stimulated with bacteria-derived factors such as lipopolysaccharide (LPS) and peptidoglycan (PG). The expression of interleukin (IL)-6 and of IL-8 was examined using the reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assays. The expression of intercellular adhesion molecule-1 (ICAM-1) and of vascular cell adhesion molecule-1 (VCAM-1) on dental pulp cells was analyzed using flow cytometry. The presence of EGCG and ECG significantly reduced, in a concentration-dependent manner, the expression of IL-6 and IL-8 in dental pulp cells exposed to LPS or PG. Increased expression of ICAM-1 and VCAM-1 on the dental pulp cells in response to bacterial components was also decreased by treatment with EGCG and ECG. These findings suggest that green tea catechins may prevent the exacerbation of pulpitis.

Authors+Show Affiliations

Department of Conservative Dentistry, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan. tadashi@dent.tokushima-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20487003

Citation

Nakanishi, Tadashi, et al. "Anti-inflammatory Effect of Catechin On Cultured Human Dental Pulp Cells Affected By Bacteria-derived Factors." European Journal of Oral Sciences, vol. 118, no. 2, 2010, pp. 145-50.
Nakanishi T, Mukai K, Yumoto H, et al. Anti-inflammatory effect of catechin on cultured human dental pulp cells affected by bacteria-derived factors. Eur J Oral Sci. 2010;118(2):145-50.
Nakanishi, T., Mukai, K., Yumoto, H., Hirao, K., Hosokawa, Y., & Matsuo, T. (2010). Anti-inflammatory effect of catechin on cultured human dental pulp cells affected by bacteria-derived factors. European Journal of Oral Sciences, 118(2), pp. 145-50. doi:10.1111/j.1600-0722.2010.00714.x.
Nakanishi T, et al. Anti-inflammatory Effect of Catechin On Cultured Human Dental Pulp Cells Affected By Bacteria-derived Factors. Eur J Oral Sci. 2010;118(2):145-50. PubMed PMID: 20487003.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-inflammatory effect of catechin on cultured human dental pulp cells affected by bacteria-derived factors. AU - Nakanishi,Tadashi, AU - Mukai,Kayo, AU - Yumoto,Hiromichi, AU - Hirao,Kouji, AU - Hosokawa,Yoshitaka, AU - Matsuo,Takashi, PY - 2010/5/22/entrez PY - 2010/5/22/pubmed PY - 2010/9/18/medline SP - 145 EP - 50 JF - European journal of oral sciences JO - Eur. J. Oral Sci. VL - 118 IS - 2 N2 - Catechins (bioactive polyphenols in green tea) are known to exhibit potent anti-inflammatory properties. However, the anti-inflammatory effects of catechins on inflamed dental pulp tissue are not known. In this study, we investigated the effect of epigallocatechin-3-gallate (EGCG) and epicatechin gallate (ECG), the major components of green tea catechins, on the expression of pro-inflammatory cytokines and adhesion molecules in human dental pulp cells stimulated with bacteria-derived factors such as lipopolysaccharide (LPS) and peptidoglycan (PG). The expression of interleukin (IL)-6 and of IL-8 was examined using the reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assays. The expression of intercellular adhesion molecule-1 (ICAM-1) and of vascular cell adhesion molecule-1 (VCAM-1) on dental pulp cells was analyzed using flow cytometry. The presence of EGCG and ECG significantly reduced, in a concentration-dependent manner, the expression of IL-6 and IL-8 in dental pulp cells exposed to LPS or PG. Increased expression of ICAM-1 and VCAM-1 on the dental pulp cells in response to bacterial components was also decreased by treatment with EGCG and ECG. These findings suggest that green tea catechins may prevent the exacerbation of pulpitis. SN - 1600-0722 UR - https://www.unboundmedicine.com/medline/citation/20487003/Anti_inflammatory_effect_of_catechin_on_cultured_human_dental_pulp_cells_affected_by_bacteria_derived_factors_ L2 - https://doi.org/10.1111/j.1600-0722.2010.00714.x DB - PRIME DP - Unbound Medicine ER -