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Traditional Chinese formula, lubricating gut pill, improves loperamide-induced rat constipation involved in enhance of Cl- secretion across distal colonic epithelium.
J Ethnopharmacol. 2010 Jul 20; 130(2):347-53.JE

Abstract

AIM OF THE STUDY

Lubricating gut pill (LGP), a traditional Chinese formula, was widely used for the treatment of chronic constipation, especially in the elderly, in China. However, it is unclear whether LGP-induced laxative and/or lubricating effect is involved in water and electrolytes transport in distal colonic epithelium.

MATERIALS AND METHODS

The present study was designed to evaluate the effect of LGP on Cl(-) secretion across rat distal colonic epithelium mounted in Ussing chambers, and on a rat constipation model induced by loperamide, respectively.

RESULTS

Application of LGP in the apical side elicited a sustained increase in short circuit current (I(SC)) response in a concentration-dependent manner. Evidence that LGP-stimulated I(SC) was due to Cl(-) secretion is based on inhibition of current by (a) a Na(+)-K(+)-2Cl(-) cotransporter inhibitor bumetanide, (b) removal of Cl(-) ions in bath solution, and (c) the cystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channel blocker DPC, suggesting that a apical cAMP-dependent Cl(-) channel was activated. LGP-stimulated I(SC) was also strongly inhibited by pretreatment with clotrimazole, indicating that the basolateral K(+) channel was also involved in maintaining this cAMP-dependent Cl(-) secretion. Pretreatment of tissues with indomethacin, but not atropine, tetrodotoxin or hexamethonium, inhibited LGP-induced response. In a rat constipation model, oral administration with LGP was significantly restored number of fecal pellets, water content and mucus secretion compared with loperamide-treated group alone.

CONCLUSIONS

LGP enhances Cl(-) secretion that is mostly mediated through the release of cyclooxygenase metabolites, by which provided an osmotic force for the subsequent laxative action observed in the rat constipation model.

Links

Publisher Full Text

Authors+Show Affiliations

Wu D
Institute of Chinese Materia Medica, Shanghai University of TCM, Shanghai, China.
Wang X
No affiliation info available
Zhou J
No affiliation info available
Yuan J
No affiliation info available
Cui B
No affiliation info available
An R
No affiliation info available
Hu Z
No affiliation info available

MeSH

AnimalsChloridesCholinergic AntagonistsColonConstipationCyclic AMPCyclooxygenase InhibitorsCystic Fibrosis Transmembrane Conductance RegulatorDefecationDisease Models, AnimalDose-Response Relationship, DrugDrugs, Chinese HerbalIntestinal MucosaIntestinal SecretionsLaxativesLoperamideMaleMedicine, Chinese TraditionalMembrane PotentialsOsmosisPotassium Channel BlockersProstaglandin-Endoperoxide SynthasesRatsRats, WistarSodium Potassium Chloride Symporter Inhibitors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20488235

Citation

Wu, Dazheng, et al. "Traditional Chinese Formula, Lubricating Gut Pill, Improves Loperamide-induced Rat Constipation Involved in Enhance of Cl- Secretion Across Distal Colonic Epithelium." Journal of Ethnopharmacology, vol. 130, no. 2, 2010, pp. 347-53.
Wu D, Wang X, Zhou J, et al. Traditional Chinese formula, lubricating gut pill, improves loperamide-induced rat constipation involved in enhance of Cl- secretion across distal colonic epithelium. J Ethnopharmacol. 2010;130(2):347-53.
Wu, D., Wang, X., Zhou, J., Yuan, J., Cui, B., An, R., & Hu, Z. (2010). Traditional Chinese formula, lubricating gut pill, improves loperamide-induced rat constipation involved in enhance of Cl- secretion across distal colonic epithelium. Journal of Ethnopharmacology, 130(2), 347-53. https://doi.org/10.1016/j.jep.2010.05.018
Wu D, et al. Traditional Chinese Formula, Lubricating Gut Pill, Improves Loperamide-induced Rat Constipation Involved in Enhance of Cl- Secretion Across Distal Colonic Epithelium. J Ethnopharmacol. 2010 Jul 20;130(2):347-53. PubMed PMID: 20488235.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Traditional Chinese formula, lubricating gut pill, improves loperamide-induced rat constipation involved in enhance of Cl- secretion across distal colonic epithelium. AU - Wu,Dazheng, AU - Wang,Xinhong, AU - Zhou,Jiyan, AU - Yuan,Jianye, AU - Cui,Bo, AU - An,Rui, AU - Hu,Zhibi, Y1 - 2010/05/19/ PY - 2010/03/08/received PY - 2010/04/28/revised PY - 2010/05/08/accepted PY - 2010/5/22/entrez PY - 2010/5/22/pubmed PY - 2010/11/5/medline SP - 347 EP - 53 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 130 IS - 2 N2 - AIM OF THE STUDY: Lubricating gut pill (LGP), a traditional Chinese formula, was widely used for the treatment of chronic constipation, especially in the elderly, in China. However, it is unclear whether LGP-induced laxative and/or lubricating effect is involved in water and electrolytes transport in distal colonic epithelium. MATERIALS AND METHODS: The present study was designed to evaluate the effect of LGP on Cl(-) secretion across rat distal colonic epithelium mounted in Ussing chambers, and on a rat constipation model induced by loperamide, respectively. RESULTS: Application of LGP in the apical side elicited a sustained increase in short circuit current (I(SC)) response in a concentration-dependent manner. Evidence that LGP-stimulated I(SC) was due to Cl(-) secretion is based on inhibition of current by (a) a Na(+)-K(+)-2Cl(-) cotransporter inhibitor bumetanide, (b) removal of Cl(-) ions in bath solution, and (c) the cystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channel blocker DPC, suggesting that a apical cAMP-dependent Cl(-) channel was activated. LGP-stimulated I(SC) was also strongly inhibited by pretreatment with clotrimazole, indicating that the basolateral K(+) channel was also involved in maintaining this cAMP-dependent Cl(-) secretion. Pretreatment of tissues with indomethacin, but not atropine, tetrodotoxin or hexamethonium, inhibited LGP-induced response. In a rat constipation model, oral administration with LGP was significantly restored number of fecal pellets, water content and mucus secretion compared with loperamide-treated group alone. CONCLUSIONS: LGP enhances Cl(-) secretion that is mostly mediated through the release of cyclooxygenase metabolites, by which provided an osmotic force for the subsequent laxative action observed in the rat constipation model. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/20488235/Traditional_Chinese_formula_lubricating_gut_pill_improves_loperamide_induced_rat_constipation_involved_in_enhance_of_Cl__secretion_across_distal_colonic_epithelium_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(10)00324-7 DB - PRIME DP - Unbound Medicine ER -