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In vitro stability of fortified ophthalmic antibiotics stored at -20 degrees C for 6 months.
Cornea 2010; 29(7):807-11C

Abstract

PURPOSE

This study evaluates the in vitro stability and sterility of fortified ophthalmic antibiotic preparations of 5% solutions (50 mg/mL) made from commercially available powders of amikacin, ceftazidime, and vancomycin stored at -20 degrees C for 6 months.

METHODS

Antibiotic solutions were prepared with aseptic techniques by reconstitution and dilution of the commercially available powders (amikacin, ceftazidime, and vancomycin) and stored at -20 degrees C. After various durations of storage, the stability of various physical and chemical properties was determined by a pH meter, an osmometer, and high-performance liquid chromatography. Microbiological activity was assayed according to the European Pharmacopoeia.

RESULTS

Solutions of 5% amikacin or ceftazidime in 0.9% sodium chloride and vancomycin in 5% dextrose were found to be physically, chemically, and microbiologically stable after storage at -20 degrees C for 190 days. A time limitation of 6 months is proposed.

CONCLUSION

Fortified ophthalmic antibiotic solutions prepared and supplied by a pharmacy can be stored in freezers at -20 degrees C for later use.

Authors+Show Affiliations

Department of Pharmacy, University Hospital of Caen, Caen, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20489598

Citation

Chédru-Legros, Valérie, et al. "In Vitro Stability of Fortified Ophthalmic Antibiotics Stored at -20 Degrees C for 6 Months." Cornea, vol. 29, no. 7, 2010, pp. 807-11.
Chédru-Legros V, Fines-Guyon M, Chérel A, et al. In vitro stability of fortified ophthalmic antibiotics stored at -20 degrees C for 6 months. Cornea. 2010;29(7):807-11.
Chédru-Legros, V., Fines-Guyon, M., Chérel, A., Perdriel, A., Albessard, F., Debruyne, D., & Mouriaux, F. (2010). In vitro stability of fortified ophthalmic antibiotics stored at -20 degrees C for 6 months. Cornea, 29(7), pp. 807-11. doi:10.1097/ICO.0b013e3181c32573.
Chédru-Legros V, et al. In Vitro Stability of Fortified Ophthalmic Antibiotics Stored at -20 Degrees C for 6 Months. Cornea. 2010;29(7):807-11. PubMed PMID: 20489598.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro stability of fortified ophthalmic antibiotics stored at -20 degrees C for 6 months. AU - Chédru-Legros,Valérie, AU - Fines-Guyon,Marguerite, AU - Chérel,Aurélie, AU - Perdriel,Agathe, AU - Albessard,Françoise, AU - Debruyne,Danièle, AU - Mouriaux,Frédéric, PY - 2010/5/22/entrez PY - 2010/5/22/pubmed PY - 2010/9/30/medline SP - 807 EP - 11 JF - Cornea JO - Cornea VL - 29 IS - 7 N2 - PURPOSE: This study evaluates the in vitro stability and sterility of fortified ophthalmic antibiotic preparations of 5% solutions (50 mg/mL) made from commercially available powders of amikacin, ceftazidime, and vancomycin stored at -20 degrees C for 6 months. METHODS: Antibiotic solutions were prepared with aseptic techniques by reconstitution and dilution of the commercially available powders (amikacin, ceftazidime, and vancomycin) and stored at -20 degrees C. After various durations of storage, the stability of various physical and chemical properties was determined by a pH meter, an osmometer, and high-performance liquid chromatography. Microbiological activity was assayed according to the European Pharmacopoeia. RESULTS: Solutions of 5% amikacin or ceftazidime in 0.9% sodium chloride and vancomycin in 5% dextrose were found to be physically, chemically, and microbiologically stable after storage at -20 degrees C for 190 days. A time limitation of 6 months is proposed. CONCLUSION: Fortified ophthalmic antibiotic solutions prepared and supplied by a pharmacy can be stored in freezers at -20 degrees C for later use. SN - 1536-4798 UR - https://www.unboundmedicine.com/medline/citation/20489598/In_vitro_stability_of_fortified_ophthalmic_antibiotics_stored_at__20_degrees_C_for_6_months_ L2 - http://Insights.ovid.com/pubmed?pmid=20489598 DB - PRIME DP - Unbound Medicine ER -