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Molecular characterization and comparative transcriptional analysis of LMW-m-type genes from wheat (Triticum aestivum L.) and Aegilops species.
Theor Appl Genet. 2010 Sep; 121(5):845-56.TA

Abstract

Twelve new LMW-GS genes were characterized from bread wheat (Triticum aestivum L.) cultivar Zhongyou 9507 and five Aegilops species by AS-PCR. These genes belong to the LMW-m type and can be classified into two subclasses designated as 1 and 2, with the latter predominant in both wheat and related wild species. Genes in the two subclasses were significantly different from each other in SNPs and InDels variations. In comparison to subclass 1, the structural features of subclass 2 differs in possessing 21 amino acid residue substitutions, two fragment deletions (each with 7 amino acid residues), and a double-residue deletion and two fragment insertions (12 and 2-5 residues). Phylogenetic analysis revealed that the two subclasses were divergent at about 6.8 MYA, earlier than the divergence of C, M, N, S(s) and U genomes. The S(s) and B genomes displayed a very close relationship, whereas the C, M, N and U genomes appeared to be related to the D genome of bread wheat. The presently characterized genes ZyLMW-m1 and ZyLMW-m2 from Zhongyou 9507 were assigned to the D genome. Moreover, these genes were expressed successfully in Escherichia coli. Their transcriptional levels during grain developmental stages detected by quantitative real-time PCR (qRT-PCR) showed that both genes started to express at 5 days post-anthesis (DPA), reaching the maximum at 14 DPA after which their expressions decreased. Furthermore, the expression level of ZyLMW-m2 genes was much higher than that of ZyLMW-m1 during all grain developmental stages, suggesting that the expression efficiency of LMW-GS genes between the two subclasses was highly discrepant.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Li XH
Key Laboratory of Genetics and Biotechnology, College of Life Science, Capital Normal University, Beijing, 100048, China.
Wang K
No affiliation info available
Wang SL
No affiliation info available
Gao LY
No affiliation info available
Xie XX
No affiliation info available
Hsam SL
No affiliation info available
Zeller FJ
No affiliation info available
Yan YM
No affiliation info available

MeSH

Amino Acid SequenceCloning, MolecularEndospermEscherichia coliEvolution, MolecularGene Expression ProfilingGene Expression Regulation, PlantGenes, PlantGlutensMethionineMolecular Sequence DataMolecular WeightPhylogenyPoaceaeProtein SubunitsRNA, MessengerReverse Transcriptase Polymerase Chain ReactionSequence Homology, Amino AcidTime FactorsTranscription, GeneticTriticum

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20490445

Citation

Li, X H., et al. "Molecular Characterization and Comparative Transcriptional Analysis of LMW-m-type Genes From Wheat (Triticum Aestivum L.) and Aegilops Species." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 121, no. 5, 2010, pp. 845-56.
Li XH, Wang K, Wang SL, et al. Molecular characterization and comparative transcriptional analysis of LMW-m-type genes from wheat (Triticum aestivum L.) and Aegilops species. Theor Appl Genet. 2010;121(5):845-56.
Li, X. H., Wang, K., Wang, S. L., Gao, L. Y., Xie, X. X., Hsam, S. L., Zeller, F. J., & Yan, Y. M. (2010). Molecular characterization and comparative transcriptional analysis of LMW-m-type genes from wheat (Triticum aestivum L.) and Aegilops species. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 121(5), 845-56. https://doi.org/10.1007/s00122-010-1354-1
Li XH, et al. Molecular Characterization and Comparative Transcriptional Analysis of LMW-m-type Genes From Wheat (Triticum Aestivum L.) and Aegilops Species. Theor Appl Genet. 2010;121(5):845-56. PubMed PMID: 20490445.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization and comparative transcriptional analysis of LMW-m-type genes from wheat (Triticum aestivum L.) and Aegilops species. AU - Li,X H, AU - Wang,K, AU - Wang,S L, AU - Gao,L Y, AU - Xie,X X, AU - Hsam,S L K, AU - Zeller,F J, AU - Yan,Y M, Y1 - 2010/05/19/ PY - 2010/02/09/received PY - 2010/05/01/accepted PY - 2010/5/22/entrez PY - 2010/5/22/pubmed PY - 2010/12/14/medline SP - 845 EP - 56 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 121 IS - 5 N2 - Twelve new LMW-GS genes were characterized from bread wheat (Triticum aestivum L.) cultivar Zhongyou 9507 and five Aegilops species by AS-PCR. These genes belong to the LMW-m type and can be classified into two subclasses designated as 1 and 2, with the latter predominant in both wheat and related wild species. Genes in the two subclasses were significantly different from each other in SNPs and InDels variations. In comparison to subclass 1, the structural features of subclass 2 differs in possessing 21 amino acid residue substitutions, two fragment deletions (each with 7 amino acid residues), and a double-residue deletion and two fragment insertions (12 and 2-5 residues). Phylogenetic analysis revealed that the two subclasses were divergent at about 6.8 MYA, earlier than the divergence of C, M, N, S(s) and U genomes. The S(s) and B genomes displayed a very close relationship, whereas the C, M, N and U genomes appeared to be related to the D genome of bread wheat. The presently characterized genes ZyLMW-m1 and ZyLMW-m2 from Zhongyou 9507 were assigned to the D genome. Moreover, these genes were expressed successfully in Escherichia coli. Their transcriptional levels during grain developmental stages detected by quantitative real-time PCR (qRT-PCR) showed that both genes started to express at 5 days post-anthesis (DPA), reaching the maximum at 14 DPA after which their expressions decreased. Furthermore, the expression level of ZyLMW-m2 genes was much higher than that of ZyLMW-m1 during all grain developmental stages, suggesting that the expression efficiency of LMW-GS genes between the two subclasses was highly discrepant. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/20490445/Molecular_characterization_and_comparative_transcriptional_analysis_of_LMW_m_type_genes_from_wheat__Triticum_aestivum_L___and_Aegilops_species_ L2 - https://dx.doi.org/10.1007/s00122-010-1354-1 DB - PRIME DP - Unbound Medicine ER -