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Unique clusters of Archaea in Salar de Huasco, an athalassohaline evaporitic basin of the Chilean Altiplano.
FEMS Microbiol Ecol. 2010 Aug; 73(2):291-302.FM

Abstract

Analyses of clone libraries from water and sediments of different sites from Salar de Huasco, a high-altitude athalassohaline wetland in the Chilean Altiplano, revealed the presence of five unique clusters of uncultured Archaea that have not been previously reported or specifically assigned. These sequences were distantly related (83-96% sequence identity) to a limited number of other clone sequences and revealed no identity to cultured Archaea. The abundance of Archaea and Bacteria was estimated using qPCR and community composition was examined through the construction of clone libraries of archaeal 16S rRNA gene. Archaea were found to be dominant over Bacteria in sediments from two saline sites (sites H4: 6.31 x 10(4) and site H6: 1.37 x 10(4) microS cm(-1)) and in one of the water samples (freshwater from site H0: 607 muS cm(-1)). Euryarchaeotal sequences were more abundant than crenarchaeotal sequences. Many of the clone sequences (52%) were similar to uncultured archaeal groups found in marine ecosystems having identity values between 99% and 97%. A major fraction of the sequences (40%) were members of Methanobacteria, while others were included in the Marine Benthic Groups B and D, the Miscellaneous Crenarchaeotic Group, the Terrestrial Miscellaneous Euryarchaeotal Group, Marine Group I and Halobacteria. The presence of uncultured archaeal groups in Salar de Huasco extends their known distribution in inland waters, providing new clues about their possible function in the environment.

Authors+Show Affiliations

Max Planck Institute for Evolutionary Biology, Plön, Germany. cdorador@uantof.clNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20491927

Citation

Dorador, Cristina, et al. "Unique Clusters of Archaea in Salar De Huasco, an Athalassohaline Evaporitic Basin of the Chilean Altiplano." FEMS Microbiology Ecology, vol. 73, no. 2, 2010, pp. 291-302.
Dorador C, Vila I, Remonsellez F, et al. Unique clusters of Archaea in Salar de Huasco, an athalassohaline evaporitic basin of the Chilean Altiplano. FEMS Microbiol Ecol. 2010;73(2):291-302.
Dorador, C., Vila, I., Remonsellez, F., Imhoff, J. F., & Witzel, K. P. (2010). Unique clusters of Archaea in Salar de Huasco, an athalassohaline evaporitic basin of the Chilean Altiplano. FEMS Microbiology Ecology, 73(2), 291-302. https://doi.org/10.1111/j.1574-6941.2010.00891.x
Dorador C, et al. Unique Clusters of Archaea in Salar De Huasco, an Athalassohaline Evaporitic Basin of the Chilean Altiplano. FEMS Microbiol Ecol. 2010;73(2):291-302. PubMed PMID: 20491927.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Unique clusters of Archaea in Salar de Huasco, an athalassohaline evaporitic basin of the Chilean Altiplano. AU - Dorador,Cristina, AU - Vila,Irma, AU - Remonsellez,Francisco, AU - Imhoff,Johannes F, AU - Witzel,Karl-Paul, Y1 - 2010/04/19/ PY - 2010/5/25/entrez PY - 2010/5/25/pubmed PY - 2010/10/12/medline SP - 291 EP - 302 JF - FEMS microbiology ecology JO - FEMS Microbiol Ecol VL - 73 IS - 2 N2 - Analyses of clone libraries from water and sediments of different sites from Salar de Huasco, a high-altitude athalassohaline wetland in the Chilean Altiplano, revealed the presence of five unique clusters of uncultured Archaea that have not been previously reported or specifically assigned. These sequences were distantly related (83-96% sequence identity) to a limited number of other clone sequences and revealed no identity to cultured Archaea. The abundance of Archaea and Bacteria was estimated using qPCR and community composition was examined through the construction of clone libraries of archaeal 16S rRNA gene. Archaea were found to be dominant over Bacteria in sediments from two saline sites (sites H4: 6.31 x 10(4) and site H6: 1.37 x 10(4) microS cm(-1)) and in one of the water samples (freshwater from site H0: 607 muS cm(-1)). Euryarchaeotal sequences were more abundant than crenarchaeotal sequences. Many of the clone sequences (52%) were similar to uncultured archaeal groups found in marine ecosystems having identity values between 99% and 97%. A major fraction of the sequences (40%) were members of Methanobacteria, while others were included in the Marine Benthic Groups B and D, the Miscellaneous Crenarchaeotic Group, the Terrestrial Miscellaneous Euryarchaeotal Group, Marine Group I and Halobacteria. The presence of uncultured archaeal groups in Salar de Huasco extends their known distribution in inland waters, providing new clues about their possible function in the environment. SN - 1574-6941 UR - https://www.unboundmedicine.com/medline/citation/20491927/Unique_clusters_of_Archaea_in_Salar_de_Huasco_an_athalassohaline_evaporitic_basin_of_the_Chilean_Altiplano_ L2 - https://academic.oup.com/femsec/article-lookup/doi/10.1111/j.1574-6941.2010.00891.x DB - PRIME DP - Unbound Medicine ER -