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In vitro potential of Ascophyllum nodosum phenolic antioxidant-mediated alpha-glucosidase and alpha-amylase inhibition.
J Food Sci. 2010 Apr; 75(3):H97-102.JF

Abstract

Ascophyllum nodosum is a brown seaweed that grows abundantly in the Northeast coastal region. In this study, the potential of A. nodosum for type 2 diabetes management through antioxidant-mediated alpha-glucosidase and alpha-amylase inhibition was investigated. After the initial screening of 4 locally harvested seaweeds, A. nodosum was chosen for its highest phenolic content and was subjected to water extraction. Among extraction ratios of 50 g to 100 to 1000 mL at room temperature, 50 g/400 mL yielded the highest phenolic content of 4.5 mg/g wet weight. For evaluation of extraction temperature ranging from 20 to 80 degrees C, 50 g/400 mL was chosen as a minimum amount of extractant. Among temperatures studied, extraction at 80 degrees C resulted in the highest total phenolic contents (4.2 mg/g wet weight). All extracts had similar levels of antioxidant activity in the range of 60% to 70% in terms of 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity. The 80 degrees C extract had the highest alpha-glucosidase and alpha-amylase inhibitory activity with IC(50) of 0.24 and 1.34 microg phenolics, respectively, compared to the IC(50) of acarbose, reference inhibitor, being 0.37 and 0.68 microg. The results show that fresh A. nodosum has strong alpha-glucosidase and mild alpha-amylase inhibitory activities that correlated with phenolic contents. This study suggests a nutraceutical potential of A. nodosum based on phytochemical antioxidant and antihyperglycemia activities.

Authors+Show Affiliations

Dept. of Nutrition and Food Sciences, Food Science & Nutrition Research Center, Univ. of Rhode Island, Kingston, RI 02881, USA. emanos@mail.uri.eduNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20492300

Citation

Apostolidis, E, and C M. Lee. "In Vitro Potential of Ascophyllum Nodosum Phenolic Antioxidant-mediated Alpha-glucosidase and Alpha-amylase Inhibition." Journal of Food Science, vol. 75, no. 3, 2010, pp. H97-102.
Apostolidis E, Lee CM. In vitro potential of Ascophyllum nodosum phenolic antioxidant-mediated alpha-glucosidase and alpha-amylase inhibition. J Food Sci. 2010;75(3):H97-102.
Apostolidis, E., & Lee, C. M. (2010). In vitro potential of Ascophyllum nodosum phenolic antioxidant-mediated alpha-glucosidase and alpha-amylase inhibition. Journal of Food Science, 75(3), H97-102. https://doi.org/10.1111/j.1750-3841.2010.01544.x
Apostolidis E, Lee CM. In Vitro Potential of Ascophyllum Nodosum Phenolic Antioxidant-mediated Alpha-glucosidase and Alpha-amylase Inhibition. J Food Sci. 2010;75(3):H97-102. PubMed PMID: 20492300.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro potential of Ascophyllum nodosum phenolic antioxidant-mediated alpha-glucosidase and alpha-amylase inhibition. AU - Apostolidis,E, AU - Lee,C M, PY - 2010/5/25/entrez PY - 2010/5/25/pubmed PY - 2010/10/28/medline SP - H97 EP - 102 JF - Journal of food science JO - J Food Sci VL - 75 IS - 3 N2 - Ascophyllum nodosum is a brown seaweed that grows abundantly in the Northeast coastal region. In this study, the potential of A. nodosum for type 2 diabetes management through antioxidant-mediated alpha-glucosidase and alpha-amylase inhibition was investigated. After the initial screening of 4 locally harvested seaweeds, A. nodosum was chosen for its highest phenolic content and was subjected to water extraction. Among extraction ratios of 50 g to 100 to 1000 mL at room temperature, 50 g/400 mL yielded the highest phenolic content of 4.5 mg/g wet weight. For evaluation of extraction temperature ranging from 20 to 80 degrees C, 50 g/400 mL was chosen as a minimum amount of extractant. Among temperatures studied, extraction at 80 degrees C resulted in the highest total phenolic contents (4.2 mg/g wet weight). All extracts had similar levels of antioxidant activity in the range of 60% to 70% in terms of 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity. The 80 degrees C extract had the highest alpha-glucosidase and alpha-amylase inhibitory activity with IC(50) of 0.24 and 1.34 microg phenolics, respectively, compared to the IC(50) of acarbose, reference inhibitor, being 0.37 and 0.68 microg. The results show that fresh A. nodosum has strong alpha-glucosidase and mild alpha-amylase inhibitory activities that correlated with phenolic contents. This study suggests a nutraceutical potential of A. nodosum based on phytochemical antioxidant and antihyperglycemia activities. SN - 1750-3841 UR - https://www.unboundmedicine.com/medline/citation/20492300/In_vitro_potential_of_Ascophyllum_nodosum_phenolic_antioxidant_mediated_alpha_glucosidase_and_alpha_amylase_inhibition_ L2 - https://doi.org/10.1111/j.1750-3841.2010.01544.x DB - PRIME DP - Unbound Medicine ER -