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Development and validation of a matrix solid-phase dispersion method to determine acrylamide in coffee and coffee substitutes.
J Food Sci. 2010 Apr; 75(3):T57-63.JF

Abstract

The present study describes the development and validation of a new method based on a matrix solid-phase dispersion (MSPD) sample preparation procedure followed by GC-MS for determination of acrylamide levels in coffee (ground coffee and brewed coffee) and coffee substitute samples. Samples were dispersed in C(18) sorbent and the mixture was further packed into a preconditioned custom-made ISOLUTE bilayered SPE column (C(18)/Multimode; 1 g + 1 g). Acrylamide was subsequently eluted with water, and then derivatized with bromine and quantified by GC-MS in SIM mode. The MSPD/GC-MS method presented a LOD of 5 microg/kg and a LOQ of 10 microg/kg. Intra and interday precisions ranged from 2% to 4% and 4% to 10%, respectively. To evaluate the performance of the method, 11 samples of ground and brewed coffee and coffee substitutes were simultaneously analyzed by the developed method and also by a previously validated method based in a liquid-extraction (LE) procedure, and the results were compared showing a high correlation between them.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Soares CM
REQUIMTE, Serviço de Bromatologia, Faculdade de Farmácia, Univ. do Porto, Rua Aníbal Cunha, 164, 4099-030 Porto, Portugal. cristina.md.soares@gmail.com
Alves RC
No affiliation info available
Casal S
No affiliation info available
Oliveira MB
No affiliation info available
Fernandes JO
No affiliation info available

MeSH

AcrylamideAnalytic Sample Preparation MethodsBeveragesCoffeaCoffeeGas Chromatography-Mass SpectrometryLimit of DetectionReproducibility of ResultsSeedsSolid Phase Extraction

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

20492315

Citation

Soares, Cristina M Dias, et al. "Development and Validation of a Matrix Solid-phase Dispersion Method to Determine Acrylamide in Coffee and Coffee Substitutes." Journal of Food Science, vol. 75, no. 3, 2010, pp. T57-63.
Soares CM, Alves RC, Casal S, et al. Development and validation of a matrix solid-phase dispersion method to determine acrylamide in coffee and coffee substitutes. J Food Sci. 2010;75(3):T57-63.
Soares, C. M., Alves, R. C., Casal, S., Oliveira, M. B., & Fernandes, J. O. (2010). Development and validation of a matrix solid-phase dispersion method to determine acrylamide in coffee and coffee substitutes. Journal of Food Science, 75(3), T57-63. https://doi.org/10.1111/j.1750-3841.2010.01545.x
Soares CM, et al. Development and Validation of a Matrix Solid-phase Dispersion Method to Determine Acrylamide in Coffee and Coffee Substitutes. J Food Sci. 2010;75(3):T57-63. PubMed PMID: 20492315.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and validation of a matrix solid-phase dispersion method to determine acrylamide in coffee and coffee substitutes. AU - Soares,Cristina M Dias, AU - Alves,Rita C, AU - Casal,Susana, AU - Oliveira,M Beatriz P P, AU - Fernandes,José Oliveira, PY - 2010/5/25/entrez PY - 2010/5/25/pubmed PY - 2010/10/28/medline SP - T57 EP - 63 JF - Journal of food science JO - J Food Sci VL - 75 IS - 3 N2 - The present study describes the development and validation of a new method based on a matrix solid-phase dispersion (MSPD) sample preparation procedure followed by GC-MS for determination of acrylamide levels in coffee (ground coffee and brewed coffee) and coffee substitute samples. Samples were dispersed in C(18) sorbent and the mixture was further packed into a preconditioned custom-made ISOLUTE bilayered SPE column (C(18)/Multimode; 1 g + 1 g). Acrylamide was subsequently eluted with water, and then derivatized with bromine and quantified by GC-MS in SIM mode. The MSPD/GC-MS method presented a LOD of 5 microg/kg and a LOQ of 10 microg/kg. Intra and interday precisions ranged from 2% to 4% and 4% to 10%, respectively. To evaluate the performance of the method, 11 samples of ground and brewed coffee and coffee substitutes were simultaneously analyzed by the developed method and also by a previously validated method based in a liquid-extraction (LE) procedure, and the results were compared showing a high correlation between them. SN - 1750-3841 UR - https://www.unboundmedicine.com/medline/citation/20492315/Development_and_validation_of_a_matrix_solid_phase_dispersion_method_to_determine_acrylamide_in_coffee_and_coffee_substitutes_ L2 - https://doi.org/10.1111/j.1750-3841.2010.01545.x DB - PRIME DP - Unbound Medicine ER -