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A novel label-free electrochemical aptasensor for thrombin based on the {nano-Au/thionine}n multilayer films as redox probes.
Anal Chim Acta. 2010 Jun 04; 668(2):171-6.AC

Abstract

Herein, a novel label-free electrochemical aptasensor based on direct immobilization of the redox probes on an electrode surface was reported. Gold electrode coated Nafion was firstly modified with redox probe-thionine (Thi) through ion exchange adsorption. Then, with the help of chemisorption and electrostatic adsorption, negatively charged nano-Au and positively charged Thi were layer-by-layer (LBL) self-assembled onto the modified electrode surface, which formed {nano-Au/Thi(+)}(n) multilayer films for improving the amount of redox probes and immobilizing thiolated thrombin aptamers (TBA). In the presence of target thrombin (TB), the TBA on the multilayer film could catch the TB onto the electrode surface, which resulted in a barrier for electro-transfer, leading to decrease of the current. The proposed method avoided the cubsome redox probe labeling process, increased the amount of redox probe and reduced the distance between the redox probe and electrode surface. Thus, the approach showed a high sensitivity and a wider linearity to TB in the range from 0.12 nM to 46 nM with a detection limit of 40 pM.

Authors+Show Affiliations

Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20493294

Citation

Yuan, Yali, et al. "A Novel Label-free Electrochemical Aptasensor for Thrombin Based On the {nano-Au/thionine}n Multilayer Films as Redox Probes." Analytica Chimica Acta, vol. 668, no. 2, 2010, pp. 171-6.
Yuan Y, Yuan R, Chai Y, et al. A novel label-free electrochemical aptasensor for thrombin based on the {nano-Au/thionine}n multilayer films as redox probes. Anal Chim Acta. 2010;668(2):171-6.
Yuan, Y., Yuan, R., Chai, Y., Zhuo, Y., Liu, Z., Mao, L., Guan, S., & Qian, X. (2010). A novel label-free electrochemical aptasensor for thrombin based on the {nano-Au/thionine}n multilayer films as redox probes. Analytica Chimica Acta, 668(2), 171-6. https://doi.org/10.1016/j.aca.2010.04.020
Yuan Y, et al. A Novel Label-free Electrochemical Aptasensor for Thrombin Based On the {nano-Au/thionine}n Multilayer Films as Redox Probes. Anal Chim Acta. 2010 Jun 4;668(2):171-6. PubMed PMID: 20493294.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel label-free electrochemical aptasensor for thrombin based on the {nano-Au/thionine}n multilayer films as redox probes. AU - Yuan,Yali, AU - Yuan,Ruo, AU - Chai,Yaqin, AU - Zhuo,Ying, AU - Liu,Zhongyuan, AU - Mao,Li, AU - Guan,Shu, AU - Qian,Xiaoqing, Y1 - 2010/04/18/ PY - 2009/08/21/received PY - 2010/04/11/revised PY - 2010/04/12/accepted PY - 2010/5/25/entrez PY - 2010/5/25/pubmed PY - 2010/8/19/medline SP - 171 EP - 6 JF - Analytica chimica acta JO - Anal Chim Acta VL - 668 IS - 2 N2 - Herein, a novel label-free electrochemical aptasensor based on direct immobilization of the redox probes on an electrode surface was reported. Gold electrode coated Nafion was firstly modified with redox probe-thionine (Thi) through ion exchange adsorption. Then, with the help of chemisorption and electrostatic adsorption, negatively charged nano-Au and positively charged Thi were layer-by-layer (LBL) self-assembled onto the modified electrode surface, which formed {nano-Au/Thi(+)}(n) multilayer films for improving the amount of redox probes and immobilizing thiolated thrombin aptamers (TBA). In the presence of target thrombin (TB), the TBA on the multilayer film could catch the TB onto the electrode surface, which resulted in a barrier for electro-transfer, leading to decrease of the current. The proposed method avoided the cubsome redox probe labeling process, increased the amount of redox probe and reduced the distance between the redox probe and electrode surface. Thus, the approach showed a high sensitivity and a wider linearity to TB in the range from 0.12 nM to 46 nM with a detection limit of 40 pM. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/20493294/A_novel_label_free_electrochemical_aptasensor_for_thrombin_based_on_the_{nano_Au/thionine}n_multilayer_films_as_redox_probes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(10)00422-8 DB - PRIME DP - Unbound Medicine ER -