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A sequence that affects the copy number and stability of pSW200 and ColE1.
J Bacteriol. 2010 Jul; 192(14):3654-60.JB

Abstract

Pantoea stewartii SW2 contains 13 plasmids. One of these plasmids, pSW200, has a replicon that resembles that of ColE1. This study demonstrates that pSW200 contains a 9-bp UP element, 5'-AAGATCTTC, which is located immediately upstream of the -35 box in the RNAII promoter. A transcriptional fusion study reveals that substituting this 9-bp sequence reduces the activity of the RNAII promoter by 78%. The same mutation also reduced the number of plasmid copies from 13 to 5, as well as the plasmid stability. When a similar sequence in a ColE1 derivative, pYCW301, is mutated, the copy number of the plasmid also declines from 34 to 16 per cell. Additionally, inserting this 9-bp sequence stabilizes an unstable pSW100 derivative, pSW142K, which also contains a replicon resembling that of ColE1, indicating the importance of this sequence in maintaining the stability of the plasmid. In conclusion, the 9-bp sequence upstream of the -35 box in the RNAII promoter is required for the efficient synthesis of RNAII and maintenance of the stability of the plasmids in the ColE1 family.

Authors+Show Affiliations

Research Center for Bacterial Pathogenesis and Department of Microbiology and Immunology, Chang-Gung University, Kwei-Shan, Taoyuan 333, Taiwan.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20494993

Citation

Wu, Ying-Chung, and Shih-Tung Liu. "A Sequence That Affects the Copy Number and Stability of pSW200 and ColE1." Journal of Bacteriology, vol. 192, no. 14, 2010, pp. 3654-60.
Wu YC, Liu ST. A sequence that affects the copy number and stability of pSW200 and ColE1. J Bacteriol. 2010;192(14):3654-60.
Wu, Y. C., & Liu, S. T. (2010). A sequence that affects the copy number and stability of pSW200 and ColE1. Journal of Bacteriology, 192(14), 3654-60. https://doi.org/10.1128/JB.00095-10
Wu YC, Liu ST. A Sequence That Affects the Copy Number and Stability of pSW200 and ColE1. J Bacteriol. 2010;192(14):3654-60. PubMed PMID: 20494993.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A sequence that affects the copy number and stability of pSW200 and ColE1. AU - Wu,Ying-Chung, AU - Liu,Shih-Tung, Y1 - 2010/05/21/ PY - 2010/5/25/entrez PY - 2010/5/25/pubmed PY - 2010/7/23/medline SP - 3654 EP - 60 JF - Journal of bacteriology JO - J. Bacteriol. VL - 192 IS - 14 N2 - Pantoea stewartii SW2 contains 13 plasmids. One of these plasmids, pSW200, has a replicon that resembles that of ColE1. This study demonstrates that pSW200 contains a 9-bp UP element, 5'-AAGATCTTC, which is located immediately upstream of the -35 box in the RNAII promoter. A transcriptional fusion study reveals that substituting this 9-bp sequence reduces the activity of the RNAII promoter by 78%. The same mutation also reduced the number of plasmid copies from 13 to 5, as well as the plasmid stability. When a similar sequence in a ColE1 derivative, pYCW301, is mutated, the copy number of the plasmid also declines from 34 to 16 per cell. Additionally, inserting this 9-bp sequence stabilizes an unstable pSW100 derivative, pSW142K, which also contains a replicon resembling that of ColE1, indicating the importance of this sequence in maintaining the stability of the plasmid. In conclusion, the 9-bp sequence upstream of the -35 box in the RNAII promoter is required for the efficient synthesis of RNAII and maintenance of the stability of the plasmids in the ColE1 family. SN - 1098-5530 UR - https://www.unboundmedicine.com/medline/citation/20494993/A_sequence_that_affects_the_copy_number_and_stability_of_pSW200_and_ColE1_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=20494993 DB - PRIME DP - Unbound Medicine ER -