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In vitro and in vivo effect of acylated and unacylated ghrelin on neonatal glucose homeostasis.
Pediatr Res. 2010 Jun; 67(6):609-13.PR

Abstract

Maintenance of normal glucose homeostasis is crucial for survival during the perinatal period. Acylated ghrelin (AG) but not unacylated ghrelin (UAG) inhibits insulin release from pancreatic islets in adult rats. Circulating AG concentrations are low in the fetus and progressively increase in the postnatal period. We tested the hypothesis that AG has insulinostatic effects in vitro and in vivo during the perinatal period. In vitro, AG (10(-10)-10(-8) M) caused a 25-53% decrease in insulin secretion by islets from 5-d-old rat pups under normo- and hyperglycemic conditions, an effect that was mediated through the growth hormone secretagogue receptor (GHSR- 1a). Ghrelin (1-5) amide, [Dap3]-octanoyl, a pentapeptide that is resistant to deacylation and binds the GHSR-1a, had similar effects at 10(-8) M. In vivo, AG, UAG, or GHRP-6 [D-Lys3], a GHSR-1a antagonist, did not affect insulin or glucagon concentrations during the first 3 h of life. In 6-d-old pups, AG, UAG, or ghrelin (1-5) amide, [Dap3]-octanoyl did not affect glucose-induced insulin or C-peptide concentrations. In summary, AG has insulinostatic effects in vitro as early as during the perinatal period. These effects could not be confirmed in vivo, possibly due to the short half-life of AG in rat neonates.

Authors+Show Affiliations

Endocrinology and Diabetes Unit, British Columbia Children's Hospital, University of British Columbia, Vancouver, British Columbia, Canada.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20496472

Citation

Ni, Hehong, et al. "In Vitro and in Vivo Effect of Acylated and Unacylated Ghrelin On Neonatal Glucose Homeostasis." Pediatric Research, vol. 67, no. 6, 2010, pp. 609-13.
Ni H, De Waele K, Walia P, et al. In vitro and in vivo effect of acylated and unacylated ghrelin on neonatal glucose homeostasis. Pediatr Res. 2010;67(6):609-13.
Ni, H., De Waele, K., Walia, P., & Chanoine, J. P. (2010). In vitro and in vivo effect of acylated and unacylated ghrelin on neonatal glucose homeostasis. Pediatric Research, 67(6), 609-13. https://doi.org/10.1203/PDR.0b013e3181da463a
Ni H, et al. In Vitro and in Vivo Effect of Acylated and Unacylated Ghrelin On Neonatal Glucose Homeostasis. Pediatr Res. 2010;67(6):609-13. PubMed PMID: 20496472.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro and in vivo effect of acylated and unacylated ghrelin on neonatal glucose homeostasis. AU - Ni,Hehong, AU - De Waele,Kathleen, AU - Walia,Pallavi, AU - Chanoine,Jean-Pierre, PY - 2010/5/25/entrez PY - 2010/5/25/pubmed PY - 2010/8/25/medline SP - 609 EP - 13 JF - Pediatric research JO - Pediatr Res VL - 67 IS - 6 N2 - Maintenance of normal glucose homeostasis is crucial for survival during the perinatal period. Acylated ghrelin (AG) but not unacylated ghrelin (UAG) inhibits insulin release from pancreatic islets in adult rats. Circulating AG concentrations are low in the fetus and progressively increase in the postnatal period. We tested the hypothesis that AG has insulinostatic effects in vitro and in vivo during the perinatal period. In vitro, AG (10(-10)-10(-8) M) caused a 25-53% decrease in insulin secretion by islets from 5-d-old rat pups under normo- and hyperglycemic conditions, an effect that was mediated through the growth hormone secretagogue receptor (GHSR- 1a). Ghrelin (1-5) amide, [Dap3]-octanoyl, a pentapeptide that is resistant to deacylation and binds the GHSR-1a, had similar effects at 10(-8) M. In vivo, AG, UAG, or GHRP-6 [D-Lys3], a GHSR-1a antagonist, did not affect insulin or glucagon concentrations during the first 3 h of life. In 6-d-old pups, AG, UAG, or ghrelin (1-5) amide, [Dap3]-octanoyl did not affect glucose-induced insulin or C-peptide concentrations. In summary, AG has insulinostatic effects in vitro as early as during the perinatal period. These effects could not be confirmed in vivo, possibly due to the short half-life of AG in rat neonates. SN - 1530-0447 UR - https://www.unboundmedicine.com/medline/citation/20496472/In_vitro_and_in_vivo_effect_of_acylated_and_unacylated_ghrelin_on_neonatal_glucose_homeostasis_ L2 - https://doi.org/10.1203/PDR.0b013e3181da463a DB - PRIME DP - Unbound Medicine ER -