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Characterization of a highly pathogenic avian influenza H5N1 virus isolated from an ostrich.
Biochem Biophys Res Commun. 2010 Jun 11; 396(4):973-7.BB

Abstract

The continued spread of a highly pathogenic avian influenza (HPAI) H5N1 virus among poultry and wild birds has posed a potential threat to human public health. An influenza pandemic happens, when a new subtype that has not previously circulated in humans emerges. Almost all of the influenza pandemics in history have originated from avian influenza viruses (AIV). Birds are significant reservoirs of influenza viruses. In the present study, we performed a survey of avian influenza virus in ostriches and H5N1 virus (A/Ostrich/SuZhou/097/03, China097) was isolated. This H5N1 virus is highly pathogenic to both chickens and mice. It is also able to replicate in the lungs of, and to cause death in, BALB/c mice following intranasal administration. It forms plaques in chicken embryo fibroblast (CEF) cells in the absence of trypsin. The hemagglutinin (HA) gene of the virus is genetically similar to A/Goose/Guangdong/1/96(H5N1) and belongs to clade 0. The HA sequence contains multiple basic amino acids adjacent to the cleavage site, a motif associated with HPAI viruses. More importantly, the existence of H5N1 isolates in ostriches highlights the potential threat of wild bird infections to veterinary and public health.

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Authors+Show Affiliations

Yang P
Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, 20 Dong-Da Street, Fengtai District, Beijing 100071, PR China.
Dongmei
No affiliation info available
Wang C
No affiliation info available
Tang C
No affiliation info available
Xing L
No affiliation info available
Luo D
No affiliation info available
Zhan Z
No affiliation info available
Duan Y
No affiliation info available
Jia W
No affiliation info available
Peng D
No affiliation info available
Liu X
No affiliation info available
Wang X
No affiliation info available

MeSH

AnimalsChick EmbryoChickensChinaHumansInfluenza A Virus, H5N1 SubtypeInfluenza in BirdsInfluenza, HumanLungMiceMice, Inbred BALB CPhylogenyStruthioniformesVirus Replication

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20497905

Citation

Yang, Penghui, et al. "Characterization of a Highly Pathogenic Avian Influenza H5N1 Virus Isolated From an Ostrich." Biochemical and Biophysical Research Communications, vol. 396, no. 4, 2010, pp. 973-7.
Yang P, Dongmei , Wang C, et al. Characterization of a highly pathogenic avian influenza H5N1 virus isolated from an ostrich. Biochem Biophys Res Commun. 2010;396(4):973-7.
Yang, P., Dongmei, ., Wang, C., Tang, C., Xing, L., Luo, D., Zhan, Z., Duan, Y., Jia, W., Peng, D., Liu, X., & Wang, X. (2010). Characterization of a highly pathogenic avian influenza H5N1 virus isolated from an ostrich. Biochemical and Biophysical Research Communications, 396(4), 973-7. https://doi.org/10.1016/j.bbrc.2010.05.035
Yang P, et al. Characterization of a Highly Pathogenic Avian Influenza H5N1 Virus Isolated From an Ostrich. Biochem Biophys Res Commun. 2010 Jun 11;396(4):973-7. PubMed PMID: 20497905.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of a highly pathogenic avian influenza H5N1 virus isolated from an ostrich. AU - Yang,Penghui, AU - Dongmei,, AU - Wang,Cheng, AU - Tang,Chong, AU - Xing,Li, AU - Luo,Deyan, AU - Zhan,Zhongpeng, AU - Duan,Yueqiang, AU - Jia,Weihong, AU - Peng,Daxin, AU - Liu,Xiufan, AU - Wang,Xiliang, Y1 - 2010/05/12/ PY - 2010/05/04/received PY - 2010/05/07/accepted PY - 2010/5/26/entrez PY - 2010/5/26/pubmed PY - 2010/7/14/medline SP - 973 EP - 7 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 396 IS - 4 N2 - The continued spread of a highly pathogenic avian influenza (HPAI) H5N1 virus among poultry and wild birds has posed a potential threat to human public health. An influenza pandemic happens, when a new subtype that has not previously circulated in humans emerges. Almost all of the influenza pandemics in history have originated from avian influenza viruses (AIV). Birds are significant reservoirs of influenza viruses. In the present study, we performed a survey of avian influenza virus in ostriches and H5N1 virus (A/Ostrich/SuZhou/097/03, China097) was isolated. This H5N1 virus is highly pathogenic to both chickens and mice. It is also able to replicate in the lungs of, and to cause death in, BALB/c mice following intranasal administration. It forms plaques in chicken embryo fibroblast (CEF) cells in the absence of trypsin. The hemagglutinin (HA) gene of the virus is genetically similar to A/Goose/Guangdong/1/96(H5N1) and belongs to clade 0. The HA sequence contains multiple basic amino acids adjacent to the cleavage site, a motif associated with HPAI viruses. More importantly, the existence of H5N1 isolates in ostriches highlights the potential threat of wild bird infections to veterinary and public health. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/20497905/Characterization_of_a_highly_pathogenic_avian_influenza_H5N1_virus_isolated_from_an_ostrich_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(10)00926-5 DB - PRIME DP - Unbound Medicine ER -