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A method to produce radiation hybrids for the D-genome chromosomes of wheat (Triticum aestivum L.).
Cytogenet Genome Res. 2010 Jul; 129(1-3):234-40.CG

Abstract

Radiation hybrid (RH) mapping is based on radiation-induced chromosome breakage rather than meiotic recombination, as a means to induce marker segregation for mapping. To date, the implementation of this mapping approach in hexaploid (Triticum aestivum L.; 2n = 6x = 42; AABBDD) and tetraploid (T. turgidum L.; 2n = 4x = 28; AABB) wheat has concentrated on the production of mapping panels for individual chromosomes. In order to extend the usefulness of this approach, we have devised a method to produce panels for the simultaneous mapping of all chromosomes of the D subgenome of hexaploid wheat. In this approach, seeds of hexaploid wheat (AABBDD) are irradiated and the surviving plants are crossed to tetraploid wheat (AABB) to produce a mapping panel based on quasi-pentaploids (AABBD). Chromosome lesions in the A and B genomes are largely masked in the quasi-pentaploids due to the presence of A- and B-genome chromosomes from the tetraploid parent. On the other hand, the chromosomes from the D-genome are present in one copy (hemizygous) and allow radiation hybrid mapping of all D-genome chromosomes simultaneously. Our analyses showed that transmission of D-genome chromosomes was apparently normal and that radiation-induced chromosome breakage along D-genome chromosomes was homogeneous. Chromosome breakage levels between D-genome chromosomes were comparable except for chromosome 6D which suffered greater chromosome breakage. These results demonstrate the feasibility of constructing D-genome radiation hybrids (DGRHs) in wheat.

Authors+Show Affiliations

Department of Crop and Soil Science, Oregon State University, Corvallis, OR 97331-3002, USA. oscar.riera@oregonstate.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

20501975

Citation

Riera-Lizarazu, O, et al. "A Method to Produce Radiation Hybrids for the D-genome Chromosomes of Wheat (Triticum Aestivum L.)." Cytogenetic and Genome Research, vol. 129, no. 1-3, 2010, pp. 234-40.
Riera-Lizarazu O, Leonard JM, Tiwari VK, et al. A method to produce radiation hybrids for the D-genome chromosomes of wheat (Triticum aestivum L.). Cytogenet Genome Res. 2010;129(1-3):234-40.
Riera-Lizarazu, O., Leonard, J. M., Tiwari, V. K., & Kianian, S. F. (2010). A method to produce radiation hybrids for the D-genome chromosomes of wheat (Triticum aestivum L.). Cytogenetic and Genome Research, 129(1-3), 234-40. https://doi.org/10.1159/000312723
Riera-Lizarazu O, et al. A Method to Produce Radiation Hybrids for the D-genome Chromosomes of Wheat (Triticum Aestivum L.). Cytogenet Genome Res. 2010;129(1-3):234-40. PubMed PMID: 20501975.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A method to produce radiation hybrids for the D-genome chromosomes of wheat (Triticum aestivum L.). AU - Riera-Lizarazu,O, AU - Leonard,J M, AU - Tiwari,V K, AU - Kianian,S F, Y1 - 2010/05/22/ PY - 2010/5/27/entrez PY - 2010/5/27/pubmed PY - 2010/8/7/medline SP - 234 EP - 40 JF - Cytogenetic and genome research JO - Cytogenet Genome Res VL - 129 IS - 1-3 N2 - Radiation hybrid (RH) mapping is based on radiation-induced chromosome breakage rather than meiotic recombination, as a means to induce marker segregation for mapping. To date, the implementation of this mapping approach in hexaploid (Triticum aestivum L.; 2n = 6x = 42; AABBDD) and tetraploid (T. turgidum L.; 2n = 4x = 28; AABB) wheat has concentrated on the production of mapping panels for individual chromosomes. In order to extend the usefulness of this approach, we have devised a method to produce panels for the simultaneous mapping of all chromosomes of the D subgenome of hexaploid wheat. In this approach, seeds of hexaploid wheat (AABBDD) are irradiated and the surviving plants are crossed to tetraploid wheat (AABB) to produce a mapping panel based on quasi-pentaploids (AABBD). Chromosome lesions in the A and B genomes are largely masked in the quasi-pentaploids due to the presence of A- and B-genome chromosomes from the tetraploid parent. On the other hand, the chromosomes from the D-genome are present in one copy (hemizygous) and allow radiation hybrid mapping of all D-genome chromosomes simultaneously. Our analyses showed that transmission of D-genome chromosomes was apparently normal and that radiation-induced chromosome breakage along D-genome chromosomes was homogeneous. Chromosome breakage levels between D-genome chromosomes were comparable except for chromosome 6D which suffered greater chromosome breakage. These results demonstrate the feasibility of constructing D-genome radiation hybrids (DGRHs) in wheat. SN - 1424-859X UR - https://www.unboundmedicine.com/medline/citation/20501975/A_method_to_produce_radiation_hybrids_for_the_D_genome_chromosomes_of_wheat__Triticum_aestivum_L___ L2 - https://www.karger.com?DOI=10.1159/000312723 DB - PRIME DP - Unbound Medicine ER -