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Generation of a mouse line expressing Cre recombinase in glycinergic interneurons.
Genesis 2010; 48(7):437-45G

Abstract

In caudal regions of the CNS, glycine constitutes the major inhibitory neurotransmitter. Here, we describe a mouse line that expresses Cre recombinase under the control of a BAC transgenic glycine transporter 2 (GlyT2) promoter fragment. Mating of GlyT2-Cre mice with the Cre reporter mouse lines Rosa26/LacZ and Rosa26/YFP and analysis of double transgenic offsprings revealed strong transgene activity in caudal regions of the central nervous system, i.e., brain stem and spinal cord. Some additional Cre expression was observed in cortical and cerebellar regions. In brain stem and spinal cord, Cre expressing cells were identified as glycinergic interneurons by staining with GlyT2- and glycine-immunoreactive antibodies; here, >80% of the glycine-immunoreactive cells expressed the Cre reporter protein. These data indicate that GlyT2-Cre mice are a useful tool for the genetic manipulation of glycinergic interneurons.

Authors+Show Affiliations

Department of Neurochemistry, Max-Planck-Institute for Brain Research, Deutschordenstrasse 46, 60529 Frankfurt, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20506101

Citation

Ishihara, Naoko, et al. "Generation of a Mouse Line Expressing Cre Recombinase in Glycinergic Interneurons." Genesis (New York, N.Y. : 2000), vol. 48, no. 7, 2010, pp. 437-45.
Ishihara N, Armsen W, Papadopoulos T, et al. Generation of a mouse line expressing Cre recombinase in glycinergic interneurons. Genesis. 2010;48(7):437-45.
Ishihara, N., Armsen, W., Papadopoulos, T., Betz, H., & Eulenburg, V. (2010). Generation of a mouse line expressing Cre recombinase in glycinergic interneurons. Genesis (New York, N.Y. : 2000), 48(7), pp. 437-45. doi:10.1002/dvg.20640.
Ishihara N, et al. Generation of a Mouse Line Expressing Cre Recombinase in Glycinergic Interneurons. Genesis. 2010;48(7):437-45. PubMed PMID: 20506101.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Generation of a mouse line expressing Cre recombinase in glycinergic interneurons. AU - Ishihara,Naoko, AU - Armsen,Wencke, AU - Papadopoulos,Theofilos, AU - Betz,Heinrich, AU - Eulenburg,Volker, PY - 2010/5/28/entrez PY - 2010/5/28/pubmed PY - 2010/11/5/medline SP - 437 EP - 45 JF - Genesis (New York, N.Y. : 2000) JO - Genesis VL - 48 IS - 7 N2 - In caudal regions of the CNS, glycine constitutes the major inhibitory neurotransmitter. Here, we describe a mouse line that expresses Cre recombinase under the control of a BAC transgenic glycine transporter 2 (GlyT2) promoter fragment. Mating of GlyT2-Cre mice with the Cre reporter mouse lines Rosa26/LacZ and Rosa26/YFP and analysis of double transgenic offsprings revealed strong transgene activity in caudal regions of the central nervous system, i.e., brain stem and spinal cord. Some additional Cre expression was observed in cortical and cerebellar regions. In brain stem and spinal cord, Cre expressing cells were identified as glycinergic interneurons by staining with GlyT2- and glycine-immunoreactive antibodies; here, >80% of the glycine-immunoreactive cells expressed the Cre reporter protein. These data indicate that GlyT2-Cre mice are a useful tool for the genetic manipulation of glycinergic interneurons. SN - 1526-968X UR - https://www.unboundmedicine.com/medline/citation/20506101/Generation_of_a_mouse_line_expressing_Cre_recombinase_in_glycinergic_interneurons_ L2 - https://doi.org/10.1002/dvg.20640 DB - PRIME DP - Unbound Medicine ER -