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Characterization of anthocyanins and proanthocyanidins in wild and domesticated Mexican blackberries (Rubus spp.).
J Agric Food Chem. 2010 Jun 23; 58(12):7458-64.JA

Abstract

This study was designed to characterize and compare wild, commercial, and noncommercial cultivated blackberry genotypes grown in Michoacan, Mexico. Six genotypes, including WB-3, WB-7, WB-10, and WB-11 (all wild blackberry types), Tupy (a commercial cultivar), and UM-601 (a cultivated breeding line), were selected and profiled for anthocyanins and proanthocyanidins by separating extracts over Amberlite XAD-7 resin and Sephadex LH-20 columns. Subsequent high-performance liquid chromatography (HPLC) and liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) analyses revealed that the major anthocyanin for all genotypes was cyanidin 3-O-glucoside. The proanthocyanidins (condensed tannins) were present in mono- to hexamer forms. Also, hydrolyzable tannins, ellagitannins, were characterized in the blackberry fruits. The average anthocyanin concentration in Sephadex LH-20 fractions was 49.2 mg/g in the commercial cultivar Tupy, while in the wild genotypes and the breeding line, the range was 361.3-494.9 mg/g (cyanidin 3-O-glucoside equivalent). The proanthocyanidin concentration varied widely among wild genotypes (417.5-1343.6 mg/g, catechin equivalent). This study demonstrated that the use of Amberlite XAD-7 followed by Sephadex LH-20 chromatography, with subsequent HPLC and LC-ESI-MS analyses, was able to effectively separate and characterize the diverse polyphenolics in blackberry genotypes. These results suggest that recommendations for dietary intake of blackberries for human health benefits need to take into account the source, because of the wide inherent variation in bioactive polyphenolic content in different blackberry genotypes.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Cuevas-Rodríguez EO
Programa de Posgrado en Alimentos del Centro de la Republica (PROPAC), Universidad Autónoma de Querétaro, Santiago de Querétaro, Querétaro, Mexico.
Yousef GG
No affiliation info available
García-Saucedo PA
No affiliation info available
López-Medina J
No affiliation info available
Paredes-López O
No affiliation info available
Lila MA
No affiliation info available

MeSH

AnthocyaninsFruitGenotypeMexicoPlant ExtractsProanthocyanidinsRosaceae

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20507066

Citation

Cuevas-Rodríguez, Edith O., et al. "Characterization of Anthocyanins and Proanthocyanidins in Wild and Domesticated Mexican Blackberries (Rubus Spp.)." Journal of Agricultural and Food Chemistry, vol. 58, no. 12, 2010, pp. 7458-64.
Cuevas-Rodríguez EO, Yousef GG, García-Saucedo PA, et al. Characterization of anthocyanins and proanthocyanidins in wild and domesticated Mexican blackberries (Rubus spp.). J Agric Food Chem. 2010;58(12):7458-64.
Cuevas-Rodríguez, E. O., Yousef, G. G., García-Saucedo, P. A., López-Medina, J., Paredes-López, O., & Lila, M. A. (2010). Characterization of anthocyanins and proanthocyanidins in wild and domesticated Mexican blackberries (Rubus spp.). Journal of Agricultural and Food Chemistry, 58(12), 7458-64. https://doi.org/10.1021/jf101485r
Cuevas-Rodríguez EO, et al. Characterization of Anthocyanins and Proanthocyanidins in Wild and Domesticated Mexican Blackberries (Rubus Spp.). J Agric Food Chem. 2010 Jun 23;58(12):7458-64. PubMed PMID: 20507066.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of anthocyanins and proanthocyanidins in wild and domesticated Mexican blackberries (Rubus spp.). AU - Cuevas-Rodríguez,Edith O, AU - Yousef,Gad G, AU - García-Saucedo,Pedro A, AU - López-Medina,José, AU - Paredes-López,Octavio, AU - Lila,Mary Ann, PY - 2010/5/29/entrez PY - 2010/5/29/pubmed PY - 2010/10/6/medline SP - 7458 EP - 64 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 58 IS - 12 N2 - This study was designed to characterize and compare wild, commercial, and noncommercial cultivated blackberry genotypes grown in Michoacan, Mexico. Six genotypes, including WB-3, WB-7, WB-10, and WB-11 (all wild blackberry types), Tupy (a commercial cultivar), and UM-601 (a cultivated breeding line), were selected and profiled for anthocyanins and proanthocyanidins by separating extracts over Amberlite XAD-7 resin and Sephadex LH-20 columns. Subsequent high-performance liquid chromatography (HPLC) and liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) analyses revealed that the major anthocyanin for all genotypes was cyanidin 3-O-glucoside. The proanthocyanidins (condensed tannins) were present in mono- to hexamer forms. Also, hydrolyzable tannins, ellagitannins, were characterized in the blackberry fruits. The average anthocyanin concentration in Sephadex LH-20 fractions was 49.2 mg/g in the commercial cultivar Tupy, while in the wild genotypes and the breeding line, the range was 361.3-494.9 mg/g (cyanidin 3-O-glucoside equivalent). The proanthocyanidin concentration varied widely among wild genotypes (417.5-1343.6 mg/g, catechin equivalent). This study demonstrated that the use of Amberlite XAD-7 followed by Sephadex LH-20 chromatography, with subsequent HPLC and LC-ESI-MS analyses, was able to effectively separate and characterize the diverse polyphenolics in blackberry genotypes. These results suggest that recommendations for dietary intake of blackberries for human health benefits need to take into account the source, because of the wide inherent variation in bioactive polyphenolic content in different blackberry genotypes. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/20507066/Characterization_of_anthocyanins_and_proanthocyanidins_in_wild_and_domesticated_Mexican_blackberries__Rubus_spp___ L2 - https://doi.org/10.1021/jf101485r DB - PRIME DP - Unbound Medicine ER -