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Silk fibroin/montmorillonite nanocomposites: effect of pH on the conformational transition and clay dispersion.
Biomacromolecules. 2010 Jul 12; 11(7):1796-801.B

Abstract

By adjusting the solution pH value below the isoelectric point (pI) of silk fibroin (SF) protein, the SF was in the cation state and it could interact strongly with unmodified anionic montmorillonite (MMT) surface. In this way, novel SF-MMT nanocomposites with good clay dispersion were successfully obtained, which were confirmed by X-ray diffraction and transmission electron microscopy. Further 1H CRAMPS and 13C CP/MAS NMR experimental results revealed that beta-sheet content of SF was remarkably enhanced for nanocomposite prepared below the pI of SF (SF-MMTA) due to the strong interaction between MMT and SF. In SF-MMTA nanocomposite, clay layers acting as an efficient nucleator could efficiently enhance the beta-sheet crystallization. On the contrary, SF preserved the native random coil conformation in SF-MMTN nanocomposites due to the weak interaction between MMT and SF. A tentative model was suggested and used to explain the mechanism of clay dispersion and conformational transition of silk protein.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Dang Q
Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry and College of Chemistry, Nankai University, Tianjin, 300071, People's Republic of China.
Lu S
No affiliation info available
Yu S
No affiliation info available
Sun P
No affiliation info available
Yuan Z
No affiliation info available

MeSH

Aluminum SilicatesBentoniteClayFibroinsHydrogen-Ion ConcentrationMagnetic Resonance SpectroscopyMicroscopy, Electron, TransmissionNanocompositesProtein ConformationProtein Structure, SecondaryX-Ray Diffraction

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20509688

Citation

Dang, Qinqin, et al. "Silk Fibroin/montmorillonite Nanocomposites: Effect of pH On the Conformational Transition and Clay Dispersion." Biomacromolecules, vol. 11, no. 7, 2010, pp. 1796-801.
Dang Q, Lu S, Yu S, et al. Silk fibroin/montmorillonite nanocomposites: effect of pH on the conformational transition and clay dispersion. Biomacromolecules. 2010;11(7):1796-801.
Dang, Q., Lu, S., Yu, S., Sun, P., & Yuan, Z. (2010). Silk fibroin/montmorillonite nanocomposites: effect of pH on the conformational transition and clay dispersion. Biomacromolecules, 11(7), 1796-801. https://doi.org/10.1021/bm1002398
Dang Q, et al. Silk Fibroin/montmorillonite Nanocomposites: Effect of pH On the Conformational Transition and Clay Dispersion. Biomacromolecules. 2010 Jul 12;11(7):1796-801. PubMed PMID: 20509688.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Silk fibroin/montmorillonite nanocomposites: effect of pH on the conformational transition and clay dispersion. AU - Dang,Qinqin, AU - Lu,Shoudong, AU - Yu,Shen, AU - Sun,Pingchuan, AU - Yuan,Zhi, PY - 2010/6/1/entrez PY - 2010/6/1/pubmed PY - 2010/12/14/medline SP - 1796 EP - 801 JF - Biomacromolecules JO - Biomacromolecules VL - 11 IS - 7 N2 - By adjusting the solution pH value below the isoelectric point (pI) of silk fibroin (SF) protein, the SF was in the cation state and it could interact strongly with unmodified anionic montmorillonite (MMT) surface. In this way, novel SF-MMT nanocomposites with good clay dispersion were successfully obtained, which were confirmed by X-ray diffraction and transmission electron microscopy. Further 1H CRAMPS and 13C CP/MAS NMR experimental results revealed that beta-sheet content of SF was remarkably enhanced for nanocomposite prepared below the pI of SF (SF-MMTA) due to the strong interaction between MMT and SF. In SF-MMTA nanocomposite, clay layers acting as an efficient nucleator could efficiently enhance the beta-sheet crystallization. On the contrary, SF preserved the native random coil conformation in SF-MMTN nanocomposites due to the weak interaction between MMT and SF. A tentative model was suggested and used to explain the mechanism of clay dispersion and conformational transition of silk protein. SN - 1526-4602 UR - https://www.unboundmedicine.com/medline/citation/20509688/Silk_fibroin/montmorillonite_nanocomposites:_effect_of_pH_on_the_conformational_transition_and_clay_dispersion_ L2 - https://doi.org/10.1021/bm1002398 DB - PRIME DP - Unbound Medicine ER -