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Seroprevalence and molecular detection of hepatitis E virus in wild boar and red deer in The Netherlands.
J Virol Methods. 2010 Sep; 168(1-2):197-206.JV

Abstract

To date, sources of hepatitis E virus (HEV) in the Netherlands, including swine and wild boar, have been identified, but no direct attribution to Dutch hepatitis E cases have been demonstrated. Other animal sources may exist. To identify these species, HEV RNA detection by RT-PCR is required, but complicated. A preselection based on serology may be useful. Therefore, wildlife species were studied by serology and molecular methods. Using a species-independent double-antigen sandwich ELISA, HEV-specific antibodies were detected in sera from 12% of 1029 wild boar (Sus scrofa scrofa), in 5% of 38 red deer (Cervus elaphus) and in none of 8 studied roe deer (Capreolus capreolus). Differences in background signals were observed between species and accounted for by fitting finite mixture distributions. HEV RNA was detected in 8% of 106 wild boars, in 15% of 39 red deer and in none of 8 roe deer. In conclusion, HEV was shown to be present in European red deer for the first time. This preselection based on species-independent serological assays may be beneficial to identify new potential animal reservoirs of HEV. The consumption of Dutch undercooked wild boar and red deer meat may lead to human exposure to HEV.

Authors+Show Affiliations

Laboratory for Zoonoses and Environmental Microbiology, Centre for Infectious Disease Control Netherlands, National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands. saskia.rutjes@rivm.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20510298

Citation

Rutjes, S A., et al. "Seroprevalence and Molecular Detection of Hepatitis E Virus in Wild Boar and Red Deer in the Netherlands." Journal of Virological Methods, vol. 168, no. 1-2, 2010, pp. 197-206.
Rutjes SA, Lodder-Verschoor F, Lodder WJ, et al. Seroprevalence and molecular detection of hepatitis E virus in wild boar and red deer in The Netherlands. J Virol Methods. 2010;168(1-2):197-206.
Rutjes, S. A., Lodder-Verschoor, F., Lodder, W. J., van der Giessen, J., Reesink, H., Bouwknegt, M., & de Roda Husman, A. M. (2010). Seroprevalence and molecular detection of hepatitis E virus in wild boar and red deer in The Netherlands. Journal of Virological Methods, 168(1-2), 197-206. https://doi.org/10.1016/j.jviromet.2010.05.014
Rutjes SA, et al. Seroprevalence and Molecular Detection of Hepatitis E Virus in Wild Boar and Red Deer in the Netherlands. J Virol Methods. 2010;168(1-2):197-206. PubMed PMID: 20510298.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Seroprevalence and molecular detection of hepatitis E virus in wild boar and red deer in The Netherlands. AU - Rutjes,S A, AU - Lodder-Verschoor,F, AU - Lodder,W J, AU - van der Giessen,J, AU - Reesink,H, AU - Bouwknegt,M, AU - de Roda Husman,A M, Y1 - 2010/05/25/ PY - 2009/12/15/received PY - 2010/05/11/revised PY - 2010/05/17/accepted PY - 2010/6/1/entrez PY - 2010/6/1/pubmed PY - 2010/11/3/medline SP - 197 EP - 206 JF - Journal of virological methods JO - J Virol Methods VL - 168 IS - 1-2 N2 - To date, sources of hepatitis E virus (HEV) in the Netherlands, including swine and wild boar, have been identified, but no direct attribution to Dutch hepatitis E cases have been demonstrated. Other animal sources may exist. To identify these species, HEV RNA detection by RT-PCR is required, but complicated. A preselection based on serology may be useful. Therefore, wildlife species were studied by serology and molecular methods. Using a species-independent double-antigen sandwich ELISA, HEV-specific antibodies were detected in sera from 12% of 1029 wild boar (Sus scrofa scrofa), in 5% of 38 red deer (Cervus elaphus) and in none of 8 studied roe deer (Capreolus capreolus). Differences in background signals were observed between species and accounted for by fitting finite mixture distributions. HEV RNA was detected in 8% of 106 wild boars, in 15% of 39 red deer and in none of 8 roe deer. In conclusion, HEV was shown to be present in European red deer for the first time. This preselection based on species-independent serological assays may be beneficial to identify new potential animal reservoirs of HEV. The consumption of Dutch undercooked wild boar and red deer meat may lead to human exposure to HEV. SN - 1879-0984 UR - https://www.unboundmedicine.com/medline/citation/20510298/Seroprevalence_and_molecular_detection_of_hepatitis_E_virus_in_wild_boar_and_red_deer_in_The_Netherlands_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-0934(10)00197-7 DB - PRIME DP - Unbound Medicine ER -