Tags

Type your tag names separated by a space and hit enter

Neuroprotective effect of osthole on MPP+-induced cytotoxicity in PC12 cells via inhibition of mitochondrial dysfunction and ROS production.
Neurochem Int. 2010 Oct; 57(3):206-15.NI

Abstract

BACKGROUND

The 1-methyl-4-phenylpyridinium ion (MPP(+)), an inhibitor of mitochondrial complex I, has been widely used as a neurotoxin because it causes a severe Parkinson's disease-like syndrome accompanied by increased levels of intracellular reactive oxygen species (ROS) and apoptotic death. In the present study, we investigated the protective effects of osthole, a coumarin compound extracted from the plant-derived medicine Cnidium monnieri, on MPP(+)-induced cytotoxicity in cultured rat adrenal pheochromocytoma (PC12) cells.

METHODS

PC12 cells were treated with MPP(+) 2h after treated with different concentrations of osthole. 24h later, the cell viability, the release of lactate dehydrogenase, the activity of caspase-3 and cytochrome c, the expression ratio of Bax/Bcl-2 and the generation of intracellular ROS were detected.

RESULTS

We found that pretreatment with osthole on PC12 cells significantly reduced the loss of cell viability, the release of lactate dehydrogenase, the activity of caspase-3 and cytochrome c, the increase in Bax/Bcl-2 ratio and the generation of intracellular ROS induced by MPP(+). Moreover, our HPLC analysis of cell extracts confirmed that extracellular osthole does penetrate the cell membrane. Thus osthole may function as an intracellular antioxidant to reduce oxidative stress induced by MPP(+).

CONCLUSIONS

Therefore, the present study supports the notion that osthole may be a promising neuroprotective agent for the treatment of neurodegenerative disorders such as Parkinson's disease.

Authors+Show Affiliations

Department of Neurosurgery, Xijing Institute of Clinical Neuroscience, Xiing Hospital, Fourth Military Medical University, Xi'an 710032, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20510317

Citation

Liu, Wen-Bo, et al. "Neuroprotective Effect of Osthole On MPP+-induced Cytotoxicity in PC12 Cells Via Inhibition of Mitochondrial Dysfunction and ROS Production." Neurochemistry International, vol. 57, no. 3, 2010, pp. 206-15.
Liu WB, Zhou J, Qu Y, et al. Neuroprotective effect of osthole on MPP+-induced cytotoxicity in PC12 cells via inhibition of mitochondrial dysfunction and ROS production. Neurochem Int. 2010;57(3):206-15.
Liu, W. B., Zhou, J., Qu, Y., Li, X., Lu, C. T., Xie, K. L., Sun, X. L., & Fei, Z. (2010). Neuroprotective effect of osthole on MPP+-induced cytotoxicity in PC12 cells via inhibition of mitochondrial dysfunction and ROS production. Neurochemistry International, 57(3), 206-15. https://doi.org/10.1016/j.neuint.2010.05.011
Liu WB, et al. Neuroprotective Effect of Osthole On MPP+-induced Cytotoxicity in PC12 Cells Via Inhibition of Mitochondrial Dysfunction and ROS Production. Neurochem Int. 2010;57(3):206-15. PubMed PMID: 20510317.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuroprotective effect of osthole on MPP+-induced cytotoxicity in PC12 cells via inhibition of mitochondrial dysfunction and ROS production. AU - Liu,Wen-Bo, AU - Zhou,Jun, AU - Qu,Yan, AU - Li,Xia, AU - Lu,Cheng-Tao, AU - Xie,Ke-Liang, AU - Sun,Xiao-Li, AU - Fei,Zhou, Y1 - 2010/05/25/ PY - 2009/11/18/received PY - 2010/05/13/revised PY - 2010/05/17/accepted PY - 2010/6/1/entrez PY - 2010/6/1/pubmed PY - 2010/11/5/medline SP - 206 EP - 15 JF - Neurochemistry international JO - Neurochem Int VL - 57 IS - 3 N2 - BACKGROUND: The 1-methyl-4-phenylpyridinium ion (MPP(+)), an inhibitor of mitochondrial complex I, has been widely used as a neurotoxin because it causes a severe Parkinson's disease-like syndrome accompanied by increased levels of intracellular reactive oxygen species (ROS) and apoptotic death. In the present study, we investigated the protective effects of osthole, a coumarin compound extracted from the plant-derived medicine Cnidium monnieri, on MPP(+)-induced cytotoxicity in cultured rat adrenal pheochromocytoma (PC12) cells. METHODS: PC12 cells were treated with MPP(+) 2h after treated with different concentrations of osthole. 24h later, the cell viability, the release of lactate dehydrogenase, the activity of caspase-3 and cytochrome c, the expression ratio of Bax/Bcl-2 and the generation of intracellular ROS were detected. RESULTS: We found that pretreatment with osthole on PC12 cells significantly reduced the loss of cell viability, the release of lactate dehydrogenase, the activity of caspase-3 and cytochrome c, the increase in Bax/Bcl-2 ratio and the generation of intracellular ROS induced by MPP(+). Moreover, our HPLC analysis of cell extracts confirmed that extracellular osthole does penetrate the cell membrane. Thus osthole may function as an intracellular antioxidant to reduce oxidative stress induced by MPP(+). CONCLUSIONS: Therefore, the present study supports the notion that osthole may be a promising neuroprotective agent for the treatment of neurodegenerative disorders such as Parkinson's disease. SN - 1872-9754 UR - https://www.unboundmedicine.com/medline/citation/20510317/Neuroprotective_effect_of_osthole_on_MPP+_induced_cytotoxicity_in_PC12_cells_via_inhibition_of_mitochondrial_dysfunction_and_ROS_production_ DB - PRIME DP - Unbound Medicine ER -