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Gas chromatography-mass spectrometric method for the screening and quantification of illicit drugs and their metabolites in human urine using solid-phase extraction and trimethylsilyl derivatization.
J Sep Sci. 2010 Jun; 33(12):1767-78.JS

Abstract

A simple and rapid GC-MS method has been developed for the screening and quantification of many illicit drugs and their metabolites in human urine by using automatic SPE and trimethylsilylation. Sixty illicit drugs, including parent drugs and their metabolites that are possibly abused in Korea, can be monitored by this method. Among them, 24 popularly abused illicit drugs were selected for quantification. Very delicate optimizations were carried out in SPE, trimethylsilylation derivatization, and GC/MS to enable such remarkable achievements. Trimethylsilylated analytes were well separated within 21 min by GC-MS. In the validation results, the LOD of all the analytes were in the range of 2-75 ng/mL. The LOQ of the quantified analytes were in the range of 5-98 ng/mL. The linearity (r(2)) of the quantified analytes ranged 0.990-1.000 in each concentration range between 10 and 1000 ng/mL. The mean recoveries ranged from 62 to 126% at three different concentrations of each analyte. The inter-day and inter-person accuracies were within -13.3 approximately 14.9%, and -10.1 approximately 13.0%, respectively, and the inter-day and inter-person precisions were less than 12.9%. The method was reliable and efficient for the screening and quantification of abused illicit drugs in routine urine analysis.

Authors+Show Affiliations

Drug Analysis Laboratory, Forensic Science Division, Banporo, Seocho-gu, Seoul, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

20512807

Citation

Cheong, Jae Chul, et al. "Gas Chromatography-mass Spectrometric Method for the Screening and Quantification of Illicit Drugs and Their Metabolites in Human Urine Using Solid-phase Extraction and Trimethylsilyl Derivatization." Journal of Separation Science, vol. 33, no. 12, 2010, pp. 1767-78.
Cheong JC, Suh SI, Ko BJ, et al. Gas chromatography-mass spectrometric method for the screening and quantification of illicit drugs and their metabolites in human urine using solid-phase extraction and trimethylsilyl derivatization. J Sep Sci. 2010;33(12):1767-78.
Cheong, J. C., Suh, S. I., Ko, B. J., Kim, J. Y., In, M. K., & Cheong, W. J. (2010). Gas chromatography-mass spectrometric method for the screening and quantification of illicit drugs and their metabolites in human urine using solid-phase extraction and trimethylsilyl derivatization. Journal of Separation Science, 33(12), 1767-78. https://doi.org/10.1002/jssc.201000087
Cheong JC, et al. Gas Chromatography-mass Spectrometric Method for the Screening and Quantification of Illicit Drugs and Their Metabolites in Human Urine Using Solid-phase Extraction and Trimethylsilyl Derivatization. J Sep Sci. 2010;33(12):1767-78. PubMed PMID: 20512807.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gas chromatography-mass spectrometric method for the screening and quantification of illicit drugs and their metabolites in human urine using solid-phase extraction and trimethylsilyl derivatization. AU - Cheong,Jae Chul, AU - Suh,Sung Ill, AU - Ko,Beom Jun, AU - Kim,Jin Young, AU - In,Moon Kyo, AU - Cheong,Won Jo, PY - 2010/6/1/entrez PY - 2010/6/1/pubmed PY - 2010/10/5/medline SP - 1767 EP - 78 JF - Journal of separation science JO - J Sep Sci VL - 33 IS - 12 N2 - A simple and rapid GC-MS method has been developed for the screening and quantification of many illicit drugs and their metabolites in human urine by using automatic SPE and trimethylsilylation. Sixty illicit drugs, including parent drugs and their metabolites that are possibly abused in Korea, can be monitored by this method. Among them, 24 popularly abused illicit drugs were selected for quantification. Very delicate optimizations were carried out in SPE, trimethylsilylation derivatization, and GC/MS to enable such remarkable achievements. Trimethylsilylated analytes were well separated within 21 min by GC-MS. In the validation results, the LOD of all the analytes were in the range of 2-75 ng/mL. The LOQ of the quantified analytes were in the range of 5-98 ng/mL. The linearity (r(2)) of the quantified analytes ranged 0.990-1.000 in each concentration range between 10 and 1000 ng/mL. The mean recoveries ranged from 62 to 126% at three different concentrations of each analyte. The inter-day and inter-person accuracies were within -13.3 approximately 14.9%, and -10.1 approximately 13.0%, respectively, and the inter-day and inter-person precisions were less than 12.9%. The method was reliable and efficient for the screening and quantification of abused illicit drugs in routine urine analysis. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/20512807/Gas_chromatography_mass_spectrometric_method_for_the_screening_and_quantification_of_illicit_drugs_and_their_metabolites_in_human_urine_using_solid_phase_extraction_and_trimethylsilyl_derivatization_ L2 - https://doi.org/10.1002/jssc.201000087 DB - PRIME DP - Unbound Medicine ER -